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Ultracytochemical Localization of ATPase during the Rapid Elongation Growth Stage in Phyllostachys edulis ‘Pachyloen’ Culms

厚壁毛竹快速高生长期竹秆ATP酶超微细胞化学定位



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,2016,36(8):1566-1574
犃犮狋犪犅狅狋.犅狅狉犲犪犾.犗犮犮犻犱犲狀狋.犛犻狀.
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G H:^ V_`abc%defghiN(犘犺狔犾犾狅狊狋犪犮犺狔狊犲犱狌犾犻狊‘Pachyloen’)jkl5mnNopqrsm:;
tu

vw

x5abn

smyn

jksmnz{|n}~€

‚ATPƒ„…abc%†‡,ˆ‰ŠNop
qjksmrab%,‹

ŒŽ

x5abn

abQ

‘

ab’“”•–—˜™šr ATPƒ›œ。
smyn

pq•,žŸ abQ•ATPƒ›œ¡š,¢£abQ•rATPƒ›œ¤š,pq,¥ab
—¦§¨©ATPƒ›œ。jksmn,pq,,žŸ  ATPƒ›œ¡pq•l,abQ、ª«¬­、b
q®¯bq°±•—˜ATPƒ›œ。{|n,²pq•,žŸ Q•˜¡³rATPƒ›œ。ATPƒ´pq
smtuµ67¶T·abh$Qrx¸z¹Qºª«

»·abhr¼{

½ºz¾¿ÀºÁ•ATPƒ›
œr´ŽÃĘÅÂ$Qr@V

pÆÇÈprpq,¦§¨©ATPƒ›œ,pÉʌabATPƒ›
œ¡l

pÆrÇÈËÌpTpqT$Qª«˜ÍŒÎrÏc

ÐÑÒpqsm5m

IJK

ghiN
;ATPƒ;l5m;No;pqsm
LMNO$
:Q944.62 !PQRS:A
犝犾狋狉犪犮狔狋狅犮犺犲犿犻犮犪犾犔狅犮犪犾犻狕犪狋犻狅狀狅犳犃犜犘犪狊犲犱狌狉犻狀犵狋犺犲犚犪狆犻犱犈犾狅狀犵犪狋犻狅狀
犌狉狅狑狋犺犛狋犪犵犲犻狀犘犺狔犾犾狅狊狋犪犮犺狔狊犲犱狌犾犻狊‘犘犪犮犺狔犾狅犲狀’犆狌犾犿狊
XUTingting,YANGGuangyao,YANGQingpei,YUFen
(JiangxiProvincialKeyLaboratoryforBambooGermplasmResourcesandUtilization,JiangxiAgriculturalUniversity,Nanchang
330045,China)
犃犫狊狋狉犪犮狋:TheultracytochemicallocalizationofATPaseduringtherapidheightgrowthstagein犘犺狔犾
犾狅狊狋犪犮犺狔狊犲犱狌犾犻狊‘Pachyloen’culmswasstudiedwithcytochemicaltechnology.Thedevelopmentofinter
nodeelongationcanbedividedintofourstages:meristematicstage,initialelongationstage,rapidelonga
tionstageandmaturitystage.Theresultsshowedthattheplasmamembrane,karyotheca,organelemem
branesystemalhadhighATPaseactivityatthemeristematicstage.Duringtheinitialelongationstage,
theplasmamembraneofthegroundtissuehadhighATPaseactivityintheupperpartoftheinternode,
whiletheshortparenchymacelshadhigherATPaseactivity.ThereisnoATPasedepositioninanycels
ofthebasicpartofinternode.Duringtherapidelongationstage,ATPaseactivityofgroundtissueinthe
basicpartofinternodewashigherthanthatintheupperpart,wheretheplasmamembrane,transportves
icle,intercelularspaceandplasmodesmaalhadATPaseactivity.Onlytheplasmamembraneofground
tissueintheupperpartofinternodehadlowATPaseactivityduringthematuritystage.Duringtheelonga
tiongrowthofinternode,ATPasewasmainlyrelatedwithsynthesisandtransportofnewcelwalmateri
als,whileATPasearoundthecrystalsandstarchgrainsoutermembraneplayedaroleinstoringmaterials.
Atthebasicpartofinternodewhichhadnodiaphragm,ATPaseactivitywasnotobserved,whilephloem
ganglionhadhighATPaseactivityinthenode.Thechangesofthestructurerelatedtomasstransportbe
tweeninternodeandnodewerecausedbythelossofdiaphragm,furthermoreinfluencedinternodeelonga
tion.
犓犲狔狑狅狉犱狊:犘犺狔犾犾狅狊狋犪犮犺狔狊犲犱狌犾犻狊‘Pachyloen’;ATPase;growthinheight;culm;theelongationgrowthof
internode
  ghiN(犘犺狔犾犾狅狊狋犪犮犺狔狊犲犱狌犾犻狊‘Pachyloen’)
ÓÔÕ:Ö´iN
(犘犺狔犾犾狅狊狋犪犮犺狔狊犲犱狌犾犻狊)×ØÏÙ
ÚÛµÜ~ÝÞßÄàáVâzãVäåræP

ĘNohg

,çYè

ãéê

뜚

5$ì
L

íîàáœð

ĘñòróVÕô
[1]。
ÔÕ
,´
ghiN675$%õœ

öã{N÷ø

5ù5cõœúûü@Vzýþÿ!

ë"œ


$ù#%œ$–%&‚“”r34
[214]。
l5mÓNÚ#$5m:;rÍ(tu

T5
$ì)*+Í

l5mnNopqjksm
,´
£
Þq,-©.Ll/

ÔÕ.Ø34Ž0q5m
Ó1ž+#$l5mrÍ(23

67>t0qx
5Ÿ r5mËÌpqsm

˜ÍNÚ#$0qx
5Ÿ z0q5mr34¡4

ˆiN

5N

6N
–78NP34¡Ø

34Ž
,´
NÚ#$l5
mtuµ0qx5Ÿ rxczx9ËÌpqs
m

0qx5Ÿ ´ã:Tp;

?@
Nãr5m

0qx5Ÿ ABCDEFpq,

[1518]。
GH˜ÍNOl5m÷ør34IJK
܆r{
[1922],
L:ÖÜMTl5m˜Ír
,2
[2025],
Nl5mtuµpqjksmrOPQ
¦R

2S

TUVWghiNNojkl5m
rab%OP34

XYZlN[ì

»N[K:
W

˜\ZlNOr5!\]z78\]Z^ù_
`a

ATPÓ5$º,$ìbcrW7$Q,de¶
Tab,¥Pfghó
。ATPƒ´5$º,ñò
xi

jcATP6<,T$Qrkª«、?lb
G

ab,$Qrü{Tx<–fg›ÿµ$ìr
ÅÂz^ó)*+Í
[2627],
2S345m:;tu
µATPƒrÞmÿ!ÏcĘnxW7rù_z
Yopq

žrZs>tfghiNjkl5mn
NoATPƒr†‡34,ÜtuvNopqj
ksmrab%,‹

1 âwz%x
1.1 TUVW
YZ â w g h i N
(犘犺狔犾犾狅狊狋犪犮犺狔狊犲犱狌犾犻狊
‘Pachyloen’)B2015y4z10{^)I!JKL
%NÚ#$PQ|

}~Ý:;€rNã

a
NãrYol/z€p

8,V‚ƒ

x„}
…3p、…9p、…15p、†、…5p(pÆÇÈ
p

¥pqzp

‡Üpqx„*}•z,

1.2 TUXY
YZ%xaˆ¬‰–
[28]
Š˜‹ÿ

Œ}â
wBŽ‘’k*{1mm×1mm×1mm“
”r¬•–U50mmol/L(pH7.2)—˜™š›œ
žŸPr4%Ø ˜¡T2.5%¢—¡r£†ž
µ
,4 ℃‘y£†3h;4 ℃‘V50mmol/L(pH
7.2)r—˜™š›œž¤¥3¦,‡¦0.5h;*
§V50mmol/L(pH7.2)r TrisMaleateœž
¤¥2¦,‡¦0.5h,4℃‘‚。?§b¨©ƒ
©;žµ©;2.5h,ƒ©;žrŸ{Y50mmol/L
(pH7.2)r TrisMaleateœžµª ATP›«
2mmol/L、Pb(NO3)23mmol/L、MgSO45mmol/L。
©;§
,´ 4℃‘¬V50mmol/L(pH7.2)rTris
Maleateœ  ž ¤ ¥ 2 ¦,‡ ¦ 0.5h;­ V
50mmol/L(pH7.2)r—˜™š›œžÝ®¤
¥2¦,‡¦0.5h;­´4℃‘,¯ B50mmol/L
(pH7.2)r—˜™š›œžŸPr1%°š£†
žµ£†t±
。50mmol/L(pH7.2)r—˜™š›
œž4℃‘¤¥2¦,‡¦20min;§VWý6¤
¥3¦,‡¦0.5h;²¤¥êrâw7³´、µ¶“
·¸6
,Epon812¹ºv»,LKBVۄ¼*½O


*½~7¾¿
,´
{À7650ÁÂ_O<…`
‘§¨ÃÄ

76518n           .//,–:ghiNjkl5mnNoATPƒ„…abc%†‡
2 YZŒ
a<=ŒÎõÅ

pqrsm:;tuXx
Yx5abn

smyn

jksmnz{|n}~


2.1 N;AB(
,žŸ abÆBx5abð!

ab‘‡B
µÇ

¾¿QT

Èɑ<

ab’ˆÊÀºz
,QËY6

˜¾¿Àxi

ÌÍⅠ,A)。´Q、
ž­

ÊÀºÁ

,Qː

‘¯ž­,rÎ
<$•—˜¡šrATPƒ›œ(ÌÍⅠ,B、C)。
2.2 Z<[(
smyn

Nopq•r,žŸ abÆB
y5h¼{n

ab,´Lìr¾¿À

ÌÍⅠ,
D)。,žŸ abrQ•˜Lì ATPƒxi,
¢ˆ£abQ•r ATPƒ›œ¡l,ÝÞma
b,rª«¬­

¾¿ÀºÁ•ˆ¯½ºÏ
ÐL˜4ìr ATPƒ›œ$QÑJ(ÌÍⅠ,D、
E)。ÒÓabQ•r ATPƒÑJ$ÔÕxi

ÌÍⅠ,F)。
Tpq•+Ö

Nopq,rm

£ab¼
!×Ù~<Ø
,´
,žŸ abzÒÓabrQ

‘

ž­•¦:֏Ⅰ,G、H)。
Nop,žŸ abrQ

‘

ª«¬­


bq°±¯½ºÁ—˜ ATPƒ›œ(ÌÍ
Ⅰ,I、J)。ÉʌabÆBy5h¼{n,´ ÉÊ
ŒabQÏИ¡šr ATPƒ›œ(ÌÍⅠ,
K)。ÙHabQ• ATPƒ›œ¡š,ž­¯
ž­,ATPƒ›œ¡³。Úbab,´۴¼
{rÜÝQ½º

¦§¨©ÍⅠ,L)。
2.3 89Z<(
jksmn

pq•r,žŸ abzÒÓ
abrabh<Þg

£ab¡mabxcu/


ßQÏÐxirª«¬­¡Ø

´,žŸ 
abQzª«¬­•—˜ ATPƒ›œ(ÌÍ
Ⅱ,A、B)。ÉÊÒÓabQ¯ª«¬­• ATP
ƒ›œ¡

ÌÍⅡ,C),ÙHabzÚbabrQ
•ATPƒ›œ¡š(ÌÍⅡ,D)。
pq,

mabrQ

ª«¬­

bq®
ÏŘATPƒ›œ;£abˆQ•ATPƒÑ
J$¡Ø

ÊÀº

ª«¬­L˜4ìÑJ

ÌÍ
Ⅱ,E)。ÉÊÒÓabQ•ATPƒ›œ¡š(Ì
ÍⅡ,F)。ÙHab,ª˜LìrÜݽº-PÜ
Ý

ÌÍⅡ,G),àá•ATPƒ›œ¡š(ÌÍⅡ,
H)。ÚbabrQzž­,rÎ<$•˜4ì
ATPƒ›œ$Q(ÌÍⅡ,I)。
Nopr,žŸ abÆBy5h¼{n

abÏИL쪫¬­xi

´ª«¬­zb
q°±•˜¡šr ATPƒ›œ,Qzž­•
ATPƒ›œ¡³(ÌÍⅡ,J)。Éʌaby5h
<âg
,´


‘

ª«¬­¯ž­•
ATPƒ›œ—¡š(ÌÍⅡ,K)。ÉÊÒÓQ•
ATPƒ›œ¡³(ÌÍⅡ,L)。
2.4 \](
{|nNopq•r,žŸ mab¦5h
âg

ab,ã$؁Î<

²´,žŸ abQ
•Â´¡³r ATPƒ›œ(ÌÍⅢ,A)。ÒÓ
ab,¦§¨© ATPƒ›œ$QÑJ(ÌÍⅢ,
B)。
No,r,žŸ abzÒÓabÆB¦5
h¼{n

,žŸ ab‘zab,Ãä$Q—I
ÏÐcxi

abq®ÏL

ÌÍⅢ,C);ÒÓab
,Lx$QIÎ<

ÌÍⅢ,D)。2Úab,—
¦˜ATPƒ›œ$QÑJ。
¦´p,žŸ ab,§¨©ƒ›œ

ÌÍⅢ,E)。ÒÓabrabh<âg,
ÄÊÀºrÒÓabQ•˜ ATPƒ›œ(ÌÍ
Ⅲ,F)。Éʌab,˜4쪫¬­zLìÛ´
:;rÜÝQ½º

abq´Lìbq°±
,´
Î
<$z¬­•˜¡³r ATPƒ›œ(ÌÍⅢ,
G)。
2.5 ^_`a^b犃犜犘>cd
2.5.1 ^e,f pÆÇÈprpq,,mab
rab‘ØÏÐcxi

˜¾¿Àºz½ºÂ´

£
ab,ž­)åæ ç

èµÇéê

ÌÍⅢ,H)。
ÒÓabab‘‘V‚Î<

µÇLž­¦¼{

´¼habzÒÓab,¦§¨© ATPƒ›œ

ÌÍⅢ,H、I);؁abrž­,˜ëðì¿$Q
ÑJ

T£ab+Ö

mabrž­,´¤Ør
ì¿$Q

ÌÍⅢ,I)。
2.5.2 ^f Noprmabž­zQ•
ATPƒ›œ¡³;£abQ• ATPƒ›œ¡š

ÌÍⅢ,J)。ÒÓabrQ、ž­•—˜¡³
rATPƒ›œ(ÌÍⅢ,K)。؁Éʌaba
b‘Èɑ<

ö֑íáÖî

´ÃQ

‘


ž­•—˜™šrATPƒ›œ(ÌÍⅢ,L)。
8651 ! " # $ % &                   36ï
3 xðTv_
Õñ34:Ö

iNpq,žŸ zÒÓ5m
:;tuµ
,´
,žŸ zÒÓaby5h¼{n



ª«¬­

ab’“”•—˜ ATPƒ›
œ
[2829]。
ÜòóYATPƒ´Q、bq°±、ª«
¬­–º“•ÑJ

67rô$Ó¶T$Qrx
¸z¹Qºª«tu

bq®zabh•r ATP
ƒ67T$QrQÁºª«˜Í

дÊÀºzõ
öº½É,JKrATPƒ÷f$QrxÌW7@V
[30]。
ž34ŒŽ

pqsmyn

pq•r ATPƒxiñò,ØxiBQzª
«¬­•

Tpq,+Ö
,ATPƒ›œ¡š。
Ž´pqsmyn

pq• ATPƒ67¶T
·abh$Qrx¸z¹Qºª«

»·abh
r¼{

ZlabhrsWœ

ÐÑÒabrs
m

´pq•,žŸ ,½ºz¾¿ÀºÁ•
L˜ATPƒ›œ,SÞ¾¿Àz½ºX$Ì©Å
Â$Q

YabsmZ^$Qz$ìr@V

jksmnpq¥abQ•—˜¡šr
ATPƒ›œ,pq,r ATPƒøÝpq•
Ü®´Qzª«¬­•xi
,´
ÊÀºÁ

b
q°±zbq®,—˜ ATPƒ›œ,TÕñ34
,žŸ  ATPƒÑJ‡+Ý[28,31],ÒÓab÷
67´Q•ÑJ

jksmn

pq ATPƒ6
7¶T¹Qºª«

ÝÞ, ATPƒTQÁºˆ
¯$Qrü{˜Í

T·abhr¼{

absm˜
)*͓

ùúû
[15,30]
´34iNpq5mtuµ:Ö

´ü†æx5Ÿ deý5Ðþr0qx5Ÿ
 
,´
pqsmyn

ÿ~pqˆ0qx5Ÿ Y6
G

pq‚0q5m

?@pqsm

pq•a
b!"x9

0qx5Ÿ CD>Bpq,


#{|$c

%&
[32]
´34iiNl5mtu
µ:Öpq‚(˜ÜMüy5x5Ÿ ý5Ðþr
)‚ab

¢´NOpqsmrtuµ*+¡mÞ
qrx9$#

£ab,)‚ab

ÝÞ

pqx5
Ÿ AB?@pqrsmÐCDE¬

x9$#L
CD-³

ž34Ž´ghiNpqsmtu
µ

pq¦§¨©
£ab
*+@¡lrab›œ
;´
jksmnpq,L
¦§¨©0qx5Ÿ 

,žŸ zÒÓab.p
q67x

¢pqsmr6723Ó,žŸ z
ÒÓabrx9zsmG/r

ghiNNo´5mtuµ701öÖpÆÇ
ÈÖî

pÆÇÈrpq,ab,¦§¨©ATP
ĄJ$

p ATPƒ67xi´,žŸ rQ


ÒÓabrQzž­¯ÉʌabQ

‘


ž­•

¢›œ—¡š

+¡BjksmnÉ
ʌab
,ATPƒ›œ—¡l。ÐjksmnÉÊ
Œabq¯T¼hab+eÆĘ¡Ørbq°
±

bq°±•ATPƒ›œ™l,abq‚)*
r$Qª«

Y,žŸ r5mZ^$Qz$ì

üBNopqrÓH2Ę34r5è%è

67
8,žŸ abxVÐ+9:;

L<˜x=rÖ
î

>ÓH2´pq;x‚Lì?èr$Q@A

²´p

ÓH2BC

xD¼{EFrŒÎ

Œˆ
Noµ$Qr?èªbzA67:5´p

[30,33]。
Éʝ´p¼{õGr

Éʌ

Œ
Î
[3436],
67‚xA

£H;ª«

pÆÇÈr
p,

Éʌabr ATPƒ›œIlLJ
Éʌ´No,$Q?è@Aª«µrW7@V

pŒÎÙ0ÏcËÌpqm/rÙ0

pÆrÇ
ÈËÌpTpqT$Qª«˜ÍŒÎrÏc

8
ÐÑÒpqsm5m

gh!P

[1] KÌL.ghiNrPQœð¯ÃM;34[D].mN:µOK+dL%
,2003.
[2] PQR.gÊiNSTy&[J].I!OK+d,1982,
1:014.
CAID G.Preliminarysurveyof犘犺狔犾犾狅狊狋犪犮犺狔狊犲犱狌犾犻狊cv.
狆犪犮犺狔犾狅犲狀[J].犑犻犪狀犵狓犻犉狅狉犲狊狋狉狔犛犮犻犲狀犮犲犪狀犱 犜犲犮犺狀狅犾狅犵狔,
1982,1:014.
[3] PQR.gÊiN¨UrZ[J].NÚ34,1984,4(2):
120121.
CAIDG.Transplantingexperimentsof犘犺狔犾犾狅狊狋犪犮犺狔狊犲犱狌犾犻狊
cv.狆犪犮犺狔犾狅犲狀[J].犚犲狊犲犪狉犮犺狅犳 犅犪犿犫狅狅,1984,4(2):
120121.
[4] VûW,XYZ,[\],–.iN·U^ÏP———gÊiN
[J].I!JKL%%&,1997,19(4):9798.
YANGGY,LIZY,DUTZ,犲狋犪犾.Newcultivarof犘犺狔犾
96518n           .//,–:ghiNjkl5mnNoATPƒ„…abc%†‡
犾狅狊狋犪犮犺狔狊犲犱狌犾犻狊———犘犺狔犾犾狅狊狋犪犮犺狔狊犲犱狌犾犻狊cv.狆犪犮犺狔犾狅犲狀[J].
犃犮狋犪犃犵狉犻犮狌犾狋狌狉犪犲犝狀犻狏犲狉狊犻狋犪狋犻狊犑犻犪狀犵狓犻犲狀狊犻狊,1997,19(4):
9798.
[5] KÌL._`iN·æPghiN[J].a=Nb>c,
2003,5(3):40.
GUO Q R.Rarenewvarietyof犘犺狔犾犾狅狊狋犪犮犺狔狊犲犱狌犾犻狊———
犘犺狔犾犾狅狊狋犪犮犺狔狊犲犱狌犾犻狊cv.狆犪犮犺狔犾狅犲狀[J].犠狅狉犾犱犅犪犿犫狅狅犪狀犱
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17518n           .//,–:ghiNjkl5mnNoATPƒ„…abc%†‡
  MC.x5ab;PC.¼hab;LPC.mab;SPC.£ab;F.ÒÓab;PG.Éʌab;ST.ÙH;CC.Úb;N.ab‘;K.‘;ER.,QË;
M.ÊÀº;V.ž­;Nu.‘Ç;TV.ª«¬­;IS.bq®;Pi.àá;Pla.bq°±;CW.abh;PP.PÜÝ;SG.¾¿À;C.½º;‘Ý
A~C.x5abn:A.x5ab;B.ab‘ďpq•,žŸ Q•ATPƒ›œ;E.pq•r£ab,¦Îq,r¼hab,ATPƒ›œ,˜¾¿À;H.pq,rÒÓab,˜å~ATPƒ›œ,˜½º;K.ÉʌabATPƒ›œ;L.ÙHabzÚbabATPƒ›œ
MiⅠ x5abnzsmynATPƒ›œ
MC.Meristematiccel;PC.Parenchymacel;LPC.Longparenchymacel;SPC.Shortparenchymacel;F.Fiber;PG.Phloemganglion;
ST.Sievetube;CC.Companioncel;N.Nucleus;K.Karyotheca;ER.Endoplasmicreticulum;M.Mitochondria;V.Vacuole;Nu.Nucleolus;TV.
Transportvesicle;IS.Intercelularspace;Pi.Pit;Pla.Plasmodesma;CW.Celwal;PP.Pprotein;SG.Starchgrain;C.Crystal;Thesameasbelow
Fig.A-C.Themeristematiccelstage:Fig.A.Meristematiccels;Fig.B.Nucleushadcleardoublekaryotheca;Fig.C.Differentkinds
oforganelemembranesystemhadhighATPaseactivity,whichasmalamountofdepositionwithinthevacuole;Fig.D-L.Theinitialelon
gationstage:Fig.D.TheplasmamembraneofthegroundtissuehadhighATPaseactivityintheupperpartofinternode;Fig.E.Therewere
undegradednucleusandcrystalsintheshortparenchymacels;Fig.F.TheplasmamembraneoffiberalsohadATPaseactivityintheupper
partofinternode;Fig.G.TheATPaseactivityandstarchgrainsinthegroundtissueinthebasicpartofinternode;Fig.H.Thefibercelshad
twodistinctnucleolusinthebasicpartofinternode;Fig.I.TheATPaseactivityintheshortparenchymacelsofthenode;Fig.J.TheAT
Paseactivityandcrystalsingroundtissue;Fig.K.TheATPaseactivityinthephloemganglion;Fig.L.TheATPaseactivityinthesievetube
celsandcompanioncels
犘犾犪狋犲Ⅰ TheATPaseactivityinthemeristematiccelstageandinitialelongationstage
2751 ! " # $ % &                   36ï
  A.pq•mabATPƒ›œ;B.pq•£abATPƒ›œ;C.pq•ÒÓabATPƒ›œ;D.pq•ÙHzÚbabATP
ƒ›œ
;E.pq,¼habATPƒ›œ;F.pq,ÒÓabATPƒ›œ;G.ÙȕxiLìPÜÝ;H.àᕠATPƒ›œ;I.ÙHa
bATPƒ›œ;J.pr¼habTÉʌabqrbq°±• ATPƒ›œ;K.Éʌabbq°±• ATPƒ›œ;L.prÒÓab
ATPƒ›œ
MiⅡ jksmnATPƒ›œ
Fig.A.TheATPaseactivityinthelongparenchymacelsintheupperpartofinternode;Fig.B.TheATPaseactivityintheshortparen
chymacelsintheupperpartofinternode;Fig.C.TheATPaseactivityinthefibercelsintheupperpartofinternode;Fig.D.TheATPaseac
tivityinthesievetubecelsandcompanioncels;Fig.E.TheATPaseactivityinthegroundtissueinthebasicpartofinternode;Fig.F.The
ATPaseactivityinthefibercelsinthebasicpartofinternode;Fig.G.TherewerealargenumberofPproteininthesieveelements;Fig.H.
TheATPaseactivityinthepit;Fig.I.TheATPaseactivityinthesieveelements;Fig.J.Theplasmodesmabetweenparenchymacelsand
phloemganglionshadATPaseactivity;Fig.K.TheplasmodesmabetweenphloemganglionshadATPaseactivity;Fig.L.TheATPaseactivity
inthefibercelsinthenode
犘犾犪狋犲Ⅱ TheATPaseactivityintherapidelongationstage
37518n           .//,–:ghiNjkl5mnNoATPƒ„…abc%†‡
  A~G.{|n:A.pq•r£abATPƒ›œ;B.pq•rÒÓabATPƒ›œ;C.pq,r¼habATPƒ›œ;D.,r
ÒÓabATPƒ›œ;E.pr¼habATPƒ›œ,4ìbq°±Â´;F.ÒÓabrabh<âg;G.ÉʌabATPƒ›œ;H~
L.pÆÇÈp:H.¼habATPƒ›œ;I.ÒÓabATPƒ›œ;J.p¼habATPƒ›œ;K.ÒÓabATPƒ›œ;L.Û´:;rÉÊ
ŒabATPƒ›œ,˜‘íáÖî
Mi Ⅲ {|nzpÆÇÈprATPƒ›œ
FigA-G.Thematuritystage:Fig.A.TheATPaseactivityintheshortparenchymacelsintheupperpartofinternode;Fig.B.TheAT
Paseactivityinthefibercelsintheupperpartofinternode;Fig.C.TheATPaseactivityintheparenchymacelsinthebasicpartofinternode;
Fig.D.TheATPaseactivityinthefibercelsinthebasicpartofinternode;Fig.E.TheATPaseactivityandlittleplasmodesmaintheparen
chymacelsinthenode;Fig.F.Thecelwalsoffibercelsthickenedsignificantly;Fig.G.TheATPaseactivityinthephloemganglions;Fig.
H-L.Theinternodewhichhadnodiaphragm:Fig.H.TheATPaseactivityintheparenchymacels;Fig.I.TheATPaseactivityinthefiber
cels;Fig.J.TheATPaseactivityintheparenchymacelsinthenode;Fig.K.TheATPaseactivityinthefibercelsinthenode;Fig.L.The
ATPaseactivityinthedevelopingphloemganglionswhichhadnuclearperforationphenomenon
犘犾犪狋犲Ⅲ TheATPaseactivityinthematuritystageandtheinternodewhichhadnodiaphragm

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