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Genetic Analysis and Gene Mapping of a Semi dwarf and Deformed Flower Mutant in Rice

一个水稻半矮化和花发育异常突变体的遗传分析和分子定位



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,2016,36(5):0910-0915
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犇犲犳狅狉犿犲犱犉犾狅狑犲狉犕狌狋犪狀狋犻狀犚犻犮犲
CHUZhizhan1,XIEYongyao1,HUChen2,GUOHaibin3,LIUYaoguang1
(1ColegeofLifeSciences,SouthChinaAgriculturalUniversity,StateKeyLaboratoryforConservationandUtilizationofSub
tropicalAgrobioresources,Guangzhou510642,China;2ColegeofFoodSciences,SouthChinaAgriculturalUniversity,Guang
zhou510642,China;3CenterofExperimentalTeachingforCommonBasicCourse,SouthChinaAgriculturalUniversity,Guang
zhou510642,China)
犃犫狊狋狉犪犮狋:Aricesemidwarfanddeformedflowermutant,temporarilynamedas狊犱犱犳3,wasidentified
from60Coγrayradiationmutationin犼犪狆狅狀犻犮犪ricevarietyNipponbare.Themutantwascharacterizedby
semidwarf,moretilers,semienclosedpanicle,stamensdysplasiaandnonpolen.Geneticsanalysisindi
catedthatthemutantwascontroledbyasinglerecessivegene.TheF2 mappingpopulationwasderived
fromacrossbetweentheheterozygous狊犱犱犳3genotypeandawidecompatiblevarietyDular.Thegene
waslocatedina227kbregionbetweentheIn/Delmarker333591and333818onchromosome3inrice,
whichwasassumedtobeanewgenecontrolingsemidwarfanddeformedflower.
犓犲狔狑狅狉犱狊:rice;semidwarfanddeformedflower;genemapping;geneticsanalysis
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typeand狊犱犱犳3:ⅠⅣ.Theupperfourinternodesdownpanicle;indicateastatisticalysignificantdifference(犘<0.01)
Fig.1 ComparisonofmorphologicalcharactersbetweenNipponbareand狊犱犱犳3
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333993 TTGCAGCTGACGATTCAACAG CCTTGAGTCTTGACAAGATGG
333440 GTAGACAAACGGTACACG TCGGTGGACATAGTTGTG
333591 GCATCCCTTTCTGTAGAGTAC TTAGTATGAAGTGAGAGGCCG
333818 AAACCCCCATCAGAACAGC AGGTCGCGCAAAACCTCAA
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[1] =ð÷,>?@.v4Àyrz撿û[M].èA:èAD%
EBåCD
,1991:69.
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犿狔狅犳犛犮犻犲狀犮犲狊狅犳狋犺犲犝狀犻狋犲犱犛狋犪狋犲狊狅犳犃犿犲狉犻犮犪,1999,96
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