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作 者 ：褚云霞1,2,3,潘俊松1,吴爱忠1,4*,蔡 润1,陈海荣2,3

期 刊 ：西北植物学报 2013年 2期 页码：422~428

关键词：矮牵牛；花色基因；分离；应用；转化；

Keywords:Petunia hybrida, anthocyanin biosynthetic genes, clone, application, transformation,

摘 要 ：矮牵牛花色素苷生物合成过程中至少有12种酶参与,除了AAT、AMT之外的大多数相关合成酶结构基因已经被克隆,调节基因An2、An4及酶活性调节基因difF也先后从矮牵牛中分离出来。多种花色基因如DFR、F3′5′H、CHS、CHI、CHR、Lc等转化矮牵牛都能影响花色,外源CHS导入还会引起雄性不育。该文主要对近年来国内外有关矮牵牛花色相关基因的分离、应用及外源基因转入三方面的研究进展进行综述,并对矮牵牛花色基因工程研究的应用前景进行了讨论。

Abstract:The research progress about anthocyanin biosynthetic genes cloning,application and exogenous gene transformation of Petunia hybrida were reviewed in this paper.At least 12 enzymes were involved in the petunia anthocyanin biosynthesis.Except AAT and AMT,the most of synthetic enzyme genes,regulatory genes An2,An4 and enzyme activity regulation gene difF,have been cloned.A variety of exogenous color genes such as DFR,F3′5′H,CHS,CHI,CHR and Lc were introduced into petunia,the changes of flower color were observed.Some researchers also reported that the transformants with CHS were male sterility.The application prospects about petunia anthocyanin biosynthetic genes were also discussed.

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