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作 者 ：陈 洁1,2，安利清2，王 涛3，姚 娜1，李潞滨1，杨 凯2*

期 刊 ：西北植物学报 2012年 8期 页码：1511~1517

关键词：查尔酮合成酶基因(CHS)；载体构建；烟草转化；类黄酮；花色改变；

Keywords:chalcone synthase gene (CHS), vector construction, tobacco transformation, flavonoids, color altered flowers,

摘 要 ：以‘西伯利亚’百合为试材，利用PCR技术克隆了查尔酮合成酶基因（CHS），构建了CHS基因的正义和反义植物表达载体，采用农杆菌介导法转化烟草叶盘，获得了转正义CHS基因的本明烟草18株，转反义CHS基因的普通烟草21株，总转化率为26.0%。高效液相色谱法（HPLC）检测结果显示，正义CHS转基因的本明烟草类黄酮含量升高14.0%~59.7%，反义CHS转基因的普通烟草类黄酮含量降低44.5%~76.4%。花色观察结果显示，正义转基因烟草的花瓣颜色未见变化，反义转基因烟草部分植株的花瓣颜色变浅。研究表明，CHS基因遗传转化是进行花色调控的有效手段之一。

Abstract:The chalcone synthase gene (CHS) was cloned from Lilium oriental hybrid ‘Siberia’ by PCR method.Sense and antisense CHS were constructed into plant expression vectors pCAMBIA1304,and then transformed into tobacco by agrobacterium mediated approach.18 plants of sense CHS transgenic Nicotiana benthamiana and 21 plants of antisense CHS transgenic Nicotiana tabacum were obtained and the total transform ratio was 26.0%.High Performance Liquid Chromatography (HPLC) suggested that CHS expression accumulation amplified by 14.0%~59.7% in N.benthamiana transformed with sense gene,and the accumulation declined by 44.5%~76.4% in N.tabacum transformed with antisence gene compared to the wild type.The petals of the transgenic N.benthamiana had no color changes,but the petal color in some of the transgenic N.tabacum plants was faded.The results indicated that the transformation of CHS gene was an effective method to flower color regulation.

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