Abstract:By biological identification and RT-PCR method it was found that Northern cereal mosaic virus (NCMV) was the pathogen of wheat rosette stunt disease. It was showed that 91.3% of the samples were positive in 367 symptomatic wheat plants collected from Hebei, Henan, Shandong and Shanxi Provinces of China in 2010. The NCMV N, P, M, G and L gene sequences were amplified and obtained by RT-PCR with the primers designed according to the target gene. BLAST analysis revealed that the identity of nucleotide sequences derived from Hebei, Henan, Shandong and Shanxi isolates was 98.9%-99.3% and greater than 99% of deduced amino acid sequences. The identity of nucleotide and deduced amino acid sequences between these Chinese and Japanese NCMV isolates was 92.2%-94% and 98.6%-99.4%, respectively. Sequence analysis showed a high level of sequence conservation among NCMV isolates. However, there was a small variation between China and Japan isolates.