---

作 者 ：谭美莲，严明芳，汪 磊，王力军，严兴初*

期 刊 ：西北植物学报 2012年 8期 页码：1539~1546

关键词：蓖麻；SSR；杂种鉴定；遗传变异；遗传多样性；

Keywords:Castor bean, SSR, hybrid identification, genetic variation, genetic diversity,

摘 要 ：采用SSR标记对蓖麻CSR24×CSR181杂交所得的F₁种子进行分析，为蓖麻早期杂种鉴定和遗传变异分析奠定技术与理论基础。结果表明：（1）各位点鉴定所得结果基本一致，除RCM207和RC129位点鉴定的杂种率未超过10%外，其它位点的杂种率都十分接近，在13.46%~17.27%之间。（2）少数个体在相关位点发生了变异，在引物RC242的扩增图谱中有4个单株出现了双亲特异条带的缺失，产生了双亲都没有的新条带；在引物Rco23、Rco26、Rco29、RC129、RCM613和RCM999的扩增结果中出现了父本特异条带的缺失，同时产生了一条新条带。（3）多样性及UPGMA聚类分析表明杂交后代遗传变异显著，子代个体与亲本间的遗传相似系数在0.45~1.0之间，个体间差异明显。

Abstract:In order to identify the hybrid purity of castor bean in early seedling stage or before sowing,castor bean hybrid verification and genetic diversity of F₁ seeds derived from CSR24×CSR181 hybridization were performed by SSR markers.It was showed that the results by different loci were basically consistent,and the hybrid rates estimated by most loci were from 13.46% to 17.27%,only which evaluated by RCM207 and RC129 were less than 10%.The variation was occurred on some loci of several individuals,4 progenies had non parent markers and generated two new bands in primer RC242;the paternal special bands were absent and a new band was appeared in some progenies in primer Rco23,Rco26,Rco29,RC129,RCM613 and RCM999.The genetic diversity and cluster analysis of SSR data indicated that the distinct differentiation existed in F₁ progenies,the genetic variation was obvious,and genetic similarity coefficient between parents and progenies was 0.45~1.0.It was provided a method for early verification of true hybrids and offered the theoretical reference for genetic analysis of offsprings.

---