Abstract:In this study,the germination of the seeds in mature capsules of Phaius tankervilleae and the developmental pathway of its protocorms were investigated.The orthogonal design was utilized to study the effect factors on multiplication of protocorms.The results showed that the seeds germination rate decreased during low temperature storage.The more the cold storage time was prolonged,the more the seeds germination rate was declined.In addition,seeds treated with 0.5% NaClO,had a higher germination rate and needed a shorter initial germination time compared to the control.The developmental pathway of embryo was as following:embryo in seed turn green before breaking through the seed coat,then broke the seed coat from one side of the seed and turn protocorms,the protocorms then developed into the seedlings.6 BA had a significant effect on the multiplication of protocorms and the optimum culture medium for multiplication of protocorms was 1/2MS+3.0 mg/L 6 BA+1.0 mg/L KT+1.0 mg/L NAA,and the mean generation multiple was 6.67.