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作 者 ：白鹏飞，杨 倩，康振生，郭 军*

期 刊 ：西北植物学报 2012年 11期 页码：2151~2156

关键词：条锈菌；电子克隆；LSD1型锌指蛋白；TaLOL2；

Keywords:stripe rust fungus, in silico cloning, LSD1-type zinc finger protein, TaLOL2,

摘 要 ：通过电子克隆与RT-PCR相结合的方法，在条锈菌诱导的小麦叶片中克隆获得1个新的LSD1型锌指蛋白基因TaLOL2，并用qRT-PCR技术分析了其转录表达特征。结果显示：（1）小麦锌指蛋白基因TaLOL2的cDNA全长1 095 bp，编码179个氨基酸。（2）TaLOL2含有3个典型的zf-LSD1型（CxxCxRxxLMYxxGASxVxCxxC）保守结构域，与水稻、拟南芥、大麦等植物LSD1型锌指蛋白序列具有高度相似性，其中与水稻OsLOL2相似度达86.0%。（3）进化树分析表明，TaLOL2与水稻、拟南芥和大麦中部分含有3个保守zf-LSD1锌指结构的基因亲缘关系较近，而与其它包含不同数目的zf-LSD1锌指结构的基因亲缘关系较远。（4）qRT-PCR定量分析表明，TaLOL2在条锈菌侵染前期呈上调表达，在亲和及非亲和反应中差异表达。研究表明，TaLOL2参与了条锈菌诱导的小麦抗病防卫反应，很可能作为正调控因子参与了小麦 条锈菌非亲和互作中对条锈菌的抗性信号途径。

Abstract:A LSD1-type zinc finger protein gene,TaLOL2,was cloned from the wheat leaves infected by stripe rust fungus (Puccinia striiformis f.sp.tritici,Pst) using in silico cloning and RT PCR and its expression pattern was analyzed through qRT-PCR.(1)The full cDNA length of TaLOL2 was 1 095 bp,which encodes a 179 amino acid protein.(2)Analysis of the protein domain features indicated that TaLOL2 contains three internally conserved zf-LSD1 domains defined as CxxCxRxxLMYxxGASxVxCxxC.Multi-sequence alignment showed that TaLOL2 shares high similarity with other LSD1-type proteins in rice (Oryza sativa),Arabidopsis thaliana and barley (Hordeum vulgare),and is 86.0% identical to the rice OsLOL2.(3)Phylogenetic analysis of the zf-LSD1-containing proteins in plants demonstrated that TaLOL2 clusters with three zf-LSD1 containing proteins and shares highly homology with OsLOL2 and HvLSD1-like1.(4)Transcriptional analysis of TaLOL2 suggested that TaLOL2 was highly induced by Pst during the early infection stage of stripe rust fungus and differentially expressed during incompatible and compatible interactions between wheat and Pst.The results suggested that TaLOL2 is involved in the wheat resistance against stripe rust fungus and probably plays a role in the incompatible interaction between wheat and Pst.

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