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作 者 ：杨小林¹，²，戚华雄³，殷得所³，曾凡松²，张舒²，喻大昭²

期 刊 ：植物病理学报 2012年 42卷 6期 页码：600-607

关键词：水稻；稻瘟病；DH群体；QTL；

Keywords:rice (Oryza sativa L.), Magnaporthe oryzae, DH population, QTL,

摘 要 ：稻瘟病是危害水稻最严重的病害之一。以抗稻瘟病的云南省地方品种魔王谷（MWG）和感稻瘟病的湖北省审定品种鄂晚8号（EW8）为亲本材料，构建双单倍体分离群体（DH）。利用22个菌株对亲本材料MWG/EW8进行致病性鉴定，从中筛选到5个毒性不同的鉴别菌株用于考察DH群体的稻瘟病抗性，构建包含120对SSR标记的分子遗传连锁图进行数量性状位点（QTL）的分析，鉴定出3个抗性QTL，均位于第6染色体长臂RM541附近， 3个QTL对抗病表型的贡献率介于7.7%~15.2 %之间，3个QTL的抗病等位基因均源自亲本MWG。

Abstract:Rice blast is one of the destructive diseases in rice. A doubled haploid (DH) population derived from a cross between Mowanggu, a famous resistant cultivar to rice blast from Yun’nan Province, and E’wan8, a susceptible cultivar from Hubei Province. 5 isolates were screened among 22 isolates, which could test insusceptible/susceptible reaction in Mowanggu/E’wan8, respectively. The resistance to rice blast of the DH population was monitored with the 5 isolates. Quantitative trait locus (QTL) was detected using a genetic map including 120 SSR. 3 QTLs were identified and linked to SSR marker RM541 in rice chromosome 6 with variance explained between 7.7%-15.2%. The 3 resistance QTLs were donated by Mowanggu.

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