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作 者 ：于永明 1，王军辉 2*，马建伟 1，张宋智 1，李平英 1，韩云花 1，魏秀琴 1

期 刊 ：西北植物学报 2012年 1期 页码：199~204

关键词：楸树；无性系；离体培养；方差分析；

Keywords:Catalpa bungei, clone, in vitro culture, variance analysis,

摘 要 ：对选育的5个楸树无性系（004-1、1-3、2-6、015-1、1-4）进行组织培养比较研究,以明确楸树无性系再生芽增殖和生根培养中遗传因素及外界条件的影响。结果表明：楸树不同无性系是影响瓶苗生长的主要因素，继代培养无性系间的方差分量在93.89%~98.16%，芽增殖系数、增殖芽数、芽长、叶数、茎段基部愈伤组织横向膨大、茎段基部愈伤组织纵向膨大在不同无性系间达到极显著水平；生根率、生根数和根长无性系间差异极显著，方差分量分别为92.97%、88.75%、96.25%。5个无性系生长性状以004-1表现最好，增殖系数为10.74，生根率为61.11%，移栽成活率为74.44%，无性系1-4最弱。

Abstract:In order to accelerate propagation of superior clones of Catalpa bungei,tissue culture of five clones was studied comparatively,which were 004-1,1-3,2-6,015-1 and 1-4.The results showed that the different clones of Catalpa bungei were the main factors influencing the growth traits of seedling,and the variance components of subculture were during 93.89%~98.16%.Variance analysis showed that the index in different clones were obvious,such as multiplication coefficient,bud number,bud length,leaf number,callus enlargement of the basal stem,and in rooting culture phase.The index such as rooting rate,number of roots,root length,and the variance components were at 0.01 significant level,and variance components were 92.97%,88.75% and 96.25%,respectively.The results indicated that 004-1 was the best of the five clones and the poorest was 1-4.As for 004-1,the multiplication coefficient reached 10.74,the rooting rate was 61.11%,the transplanting survival rate was 74.44%.

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