Abstract:The identification and evaluation of 24 tobacco varieties resistant to Tobacco mosaic virus (TMV) and Cucumber mosaic virus (CMV) were carried out by artificial inoculation in the field from 2010 to 2011. The results showed that 7 varieties, Coker86, Jiyan5, Coker176, CV87, Liaoyan8, CV91 and Zhongyan90, displayed resistance to TMV; 3 varieties, including Qinyan98, Shuangkang70 and C151 displayed moderate resistance to TMV; 7 varieties including Qinyan96, G80, Jinxing6007, Longjiang981, K326, Qinyan201 and NC89 displayed moderate susceptible to TMV, and 7 varieties, including G28, Yunyan97, Jingyehuang, Honghuadajinyuan, RG11, Yunyan85 and Yunyan87 susceptible to TMV. The 5 varieties resistant to CMV consisted of Coker86, Longjiang981, C151, Qinyan201 and Yunyan87; only Jinxing6007 was moderate resistance to CMV; 9 varieties including CV91, RG11, Coker176 , Zhongyan90, K326, Honghuadajinyuan, Jingyehuang , G80 and G28 displayed moderate susceptibility to CMV; 9 varieties, Qinyan98, Yunyan85, Qinyan96, NC89, Shuangkang70, Yunyan97, CV87, Liaoyan8 and Jiyan5, were susceptible to CMV. In this study, 2 varieties, Coker86 and C151, were resistant to both virus diseases. In addition, it was found that different resistant of tobacco varieties had different impacts on output and quality of tobacco. The study evalu-ated 24 tobacco varieties for resistance to the two viruses and the results could be utilized not only to provide the basis for variety selection and deployment, but also as parent materials in breeding for resistance.