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作 者 ：王肖红1,2,曾爱松1,高 兵1,宋立晓1,严继勇1*

期 刊 ：西北植物学报 2013年 5期 页码：885~891

关键词：结球甘蓝；StP5CS基因；遗传转化；耐盐性；

Keywords:cabbage (Brassica oleracea L.var.capitata L.), StP5CS gene, transformation, salt-tolerance,

摘 要 ：为研究StP5CS基因在结球甘蓝中的耐盐作用,以结球甘蓝下胚轴为外植体,采用农杆菌介导法将耐盐基因StP5CS和抗除草剂Bar基因导入结球甘蓝基因组中,在双丙氨膦的筛选下扩繁、生根,共获得了36株抗性植株。PCR扩增和印迹杂交检测表明:目的基因StP5CS和Bar基因已经成功导入结球甘蓝基因组中。RT-PCR检测表明:StP5CS基因在转录水平也有表达。转基因植株耐盐试验结果显示:高浓度盐处理（400 mmol/L NaCl）下,对照植株整株枯死,而转基因植株仍能正常生长;转基因植株的SOD活性、脯氨酸含量和相对膜透性均随盐浓度的升高呈上升趋势,均在400 mmol/L NaCl处理下达到最大。结果表明转基因植株对高盐环境有一定的耐受性。

Abstract:In order to study the possibility of utilizing StP5CS gene to enhance the resistance to salt,we introduced StP5CS gene into hypocotyls of cabbage (Brassica oleracea L.var.capitata L.) by Agrobacterium-mediated transformation.After selection with bialaphos,36 transgenic plants were regenerated.PCR and Southern hybridization analysis revealed that StP5CS gene had been integrated into the genome of cabbage plants.Moreover,RT-PCR analysis showed that StP5CS gene was expressed in the most transgenic plants.The results of NaCl treatment indicated that the transgenic plants could survive while CK could not.The SOD activity,proline content and rate of electrolyte leakage of transgenic plants increased with the increase of the NaCl concentration.They were highest when the transgenic plants were treated with 400 mmol/L NaCl.The results suggested that the salt tolerance of the transgenic plants increased.

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