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作 者 ：刘玉皎 1,2，侯万伟 1,2，石建斌 3

期 刊 ：西北植物学报 2012年 1期 页码：54~59

关键词：蚕豆；蛋白亚基；多样性；种质鉴定；

Keywords:faba bean, diversity, protein subunit, gene identification,

摘 要 ：采用SDS-PAGE方法，对112份不同基因型蚕豆的清蛋白和球蛋白亚基的差异性进行分析。结果显示：(1)蚕豆清蛋白和球蛋白亚基的有效等位变异分别为1.750 0±0.452 3、1.545 5±0.522 2，多态性比率分别为75.00％、54.55％，清蛋白的亚基遗传多样性指数较球蛋白亚基高。(2)蚕豆清蛋白和球蛋白分别含有在不同基因型蚕豆中构成不同的12和10个亚基，其中清蛋白含有9个基本亚基，116 kD、96 kD、45 kD为清蛋白的3个特异亚基；球蛋白含有8个基本亚基，58 kD、35 kD为球蛋白的2个特异亚基；研究共鉴定筛选出42个含有清蛋白特异亚基和21个含有球蛋白亚基的种质资源。(3)清蛋白的97 kD、63 kD基本亚基和球蛋白的97 kD、56 kD、47 kD基本亚基存在一定的缺失现象,共鉴定出19个清蛋白亚基和21个球蛋白亚基缺失的优异种质。研究表明，蚕豆清蛋白和球蛋白亚基构成在不同种质之间具有差异性，除基本亚基外，部分种质还含有特异亚基或缺失亚基。

Abstract:The differentce of protein subunits of 112 faba bean was analyzed by SDS-PAGE,in order to find out specific protein subunits.The result showed that:(1)the effective number of albumin and globulin alleles was 1.750 0±0.452 3,1.545 5±0.522 2,and the percentages of polymorphic subunit was 75.00%,54.55%,respectively.(2)It was difference between the albumin and globulin subunit numbers in different genotypes,containing 12 albumin subunits and 10 globulin subunits thereof the specific albumin subunits were 116 kD,96 kD,45 kD and specific globulin subunits are 58 kD,35 kD.42 varieties contain specific albumin subunit,21 varieties contain specific globulin subunit.(3)The lack subunits were 97 kD,63 kD of albumin and 97 kD,56 kD,47 kD of globulin.19 varieties were found with based albumin subunit lack and 21 varieties with globulin subunit lack.In summary,both albumin and globulin subunit numbers are different among different faba bean genotypes.Besides the based subunits,some have specific subunits and some have lack subunit.

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