摘 要 ：以商洛紫花丹参为材料，对其转录组序列SRA020132进行Blast分析，采用PCR技术克隆得到丹参2 酮戊二酸依赖性双加氧酶基因Sm2 ODD1，GenBank登录号为JN935923。Sm2 ODD1基因全长1 365 bp，包含3个外显子和2个内含子；cDNA全长1 189 bp，读码框951 bp，编码316个氨基酸残基；预测的编码蛋白具有2 酮戊二酸依赖性双加氧酶中结合2 酮戊二酸和亚铁离子的“H T D”、“H X”和“R Y S”保守基序以及“果冻状”空间结构。表达分析显示，Sm2 ODD1在丹参各个器官都表达，但表达水平具有组织特异性，在根中表达量最高，在叶中表达量最低；该基因表达明显受到MeJA、GA3和ABA的诱导，可能参与了丹参萜类代谢下游途径。
Abstract:By analyzing Transcriptome (SRA020132) sequences of Salvia miltiorrhiza from Shangluo and using the techniques of PCR,a new 2 oxoglutarate dependent dioxygenase gene was cloned from Salvia miltiorrhiza for the first time and named as Sm2 ODD1 (GenBank accession number JN935923).Sm2 ODD1 DNA consisted of 1 365 bp including 3 exons and 2 introns.The full length cDNA of Sm2 ODD1 was 1 189 bp,containing a single 951 bp opening reading frame and encoding a 316 amino acid residues.Bioinformatics analysis showed that Sm2 ODD1 contained a 2 oxoglutarate dependent dioxygenase superfamily containing “distorted jelly roll” domain and “H T D”,“H X” and “R Y S” motifs to bind 2 oxoglutarate and Fe2+.Quantitative RT PCR analysis revealed that the gene expressed in different organs.The expression in roots was observably higher than in flowers,stems and leaves.The gene could be induced by methyl jasmonate,GA3 and ABA.The results indicated that the Sm2 ODD1 might be involved in the biosynthesis of terpene in Salvia miltiorrhiza.