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作 者 ：周莲洁，杨中敏，张富春，王 艳*

期 刊 ：西北植物学报 2013年 6期 页码：1091~1097

关键词：盐穗木；HcSCL13基因；克隆；盐胁迫；表达分析；

Keywords:Halostachys caspica, HcSCL13 gene, clone, salt stress, expression analysis,

摘 要 ：利用抑制消减杂交法从藜科盐穗木属盐生植物盐穗木中分离得到了一个盐胁迫响应的表达序列标签（EST）片段，结合SMART^TM RACE技术获得了盐穗木GRAS转录因子基因的cDNA。序列分析表明，该基因全长2 090 bp，含有1 635 bp的阅读框，294 bp的5′-UTR和161 bp的3′-UTR，编码544个氨基酸，分子质量为61.503 kD，理论等电点为6.1。系统进化树和Blast同源序列比对分析结果显示，该基因编码的蛋白具有GRAS家族特有的C端保守结构域，并与葡萄GRAS家族蛋白VvSCL13聚集在一起，故将该基因命名为HcSCL13(GenBank登录号KC68640)。实时荧光定量qRT-PCR分析表明，HcSCL13基因在盐胁迫后表达呈明显上调，初步推测HcSCL13基因可能与盐穗木的耐盐性相关。

Abstract:An cDNA fragment was isolated from Halostachys caspica by suppression subtractive hybridization and its full-length cDNA with 2 090 bp was cloned by SMART^TM RACE,which consisted of a 1 635 bp open reading frame encoding 544 amimo acids with molecular weight of 61.503 kD and an isoelectric point of 6.1,a 294 bp 5′-UTR and 161 bp 3′-UTR.The deduced amino acid sequence got together with that of GRAS family protein VvSCL13 from Vitis vinifera and had a C-terminal conserved domain,so it was named HcSCL13 gene (GenBank accession number KC68640).Real-time PCR method was performed to investigate the expression profile of HcSCL13 gene under salt stress.HcSCL13 showed up-regulated expression patterns under salt treatment.Based on our results,we concluded that HcSCL13 gene might be involved in salt response and one of important components for the salt tolerant pathway in H.caspica.

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