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作 者 ：麻文俊1，张守攻1，王军辉1*，孙晓梅1，赵海祥2，宁延龙2

期 刊 ：西北植物学报 2013年 1期 页码：109~115

关键词：日本落叶松；无性系；扦插；激素；营养物质；酚酸；

Keywords:Japanese larch(Larix kaempferi), clones, cutting, endogenous hormones, nutritive material, phenolic acid,

摘 要 ：以生根能力差异较大的4个日本落叶松无性系为材料，对插穗生根过程中插穗内源激素含量和比值，以及总酚酸和营养物质含量的变化进行分析，以明确插穗不定根形成的主要内源性影响因素，为日本落叶松扦插繁殖技术提供理论依据。结果表明：（1）4个日本落叶松无性系扦插生根能力存在显著差异，‘日宽17-3-1’和‘日抚27-3-1’较易生根，其生根率达90%，而‘日草103-3-1’和‘日草82-4-3’难生根，其生根率低于20%。（2）在扦插生根过程中，插穗内源脱落酸(ABA)含量随扦插时间的延长而不断升高，并且较易生根和难生根无性系差异明显，与插穗的生根能力有较强的相关性；玉米素(Z)和总酚酸含量随扦插时间的延长而不断降低，并且对插穗生根具有明显的抑制作用；总氮含量表现出不断降低至逐渐稳定的趋势，但与插穗生根能力大小并无较强的相关性。（3）(IAA+Z)/ABA随扦插时间的延长而不断降低，且较易生根型小于难生根型。因此，在生产中以(IAA+Z)/ABA的比值和总酚酸含量大小可判断日本落叶松插穗的生根能力。

Abstract:The relations among rooting traits and contents of endogenous hormone,total phenolic acid,nutrient substance of four elite Japanese larch (Larix kaempferi) clones were studied.The results showed that:(1)There were significant differences in the rooting ability of cuttings amongst different clones.‘Rikuan17-3-1’ and ‘Rifu27-3-1’ were easy rooting clones,which the rooting rate reached 90%.While ‘Ricao103-3-1’ and ‘Ricao82-4-3’ was difficult rooting clones,which the rooting rate was lower than 20%.(2)The content of ABA in cuttings was increasing with time prolonging,and significant differences had been found between difficult rooting clones and easy rooting clones,there was a strong correlation between ability and ABA content.The content of Z and total phenolic acid was decreasing with time prolonging,and had a negative influence on the rooting.The content of total nitrogen was decreasing until stabilization with the time prolonging,while no significant relations with rooting ability.(3)(IAA＋Z)/ABA was decreased with the time prolonging,and the rate in easy rooting clones was lower than that in difficult rooting clones.(IAA＋Z)/ABA and total phenolic acid may be used as a standard to express the rooting ability of Japanese larch.

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