Abstract:Loop-mediated isothermal amplification (LAMP) is a highly sensitive and specific nucleic acid amplification method that can synthesize rapidly large amounts of DNA under isothermal conditions. The LAMP is successfully used to detect plant viruses and other pathogens. Banana streak virus (BSV) is a badnavirus that causes a viral leaf streak disease of banana and plantain (Musa spp.). Combined the sequences of ORF3 published in GenBank with specific sequence isolated from South China, two pairs of primers (B3/F3, BIP/FIP) were designed targeting the conserved region of BSV ORF3. A rapid detection of BSV was established by a loop-mediated isothermal amplification assay (LAMP) through optimizing the LAMP primers and reaction conditions such as Betaine concentration and MgSO4. BSV-LAMP reaction was carried out at 63℃ for 90 min, the amplified products were colored with SYBR Green I after completion of the reaction, so that the amplification could be detected with naked eyes. The limit of LAMP detection of BSV-infected banana geno-mic DNA was about 3.2 ng·μL-1, which was 25 times more sensitive than PCR method. The LAMP is a further extension of BSV detection and it is a simple and quick method that can detect BSV accurately for field samples. The LAMP is more sensitive than PCR method for BSV detection and mass propagation of culture materials.