---

作 者 ：马 刚,汪晓峰*

期 刊 ：西北植物学报 2013年 4期 页码：656~663

关键词：cDNA-AFLP；油菜种子；脱水耐性；RT-PCR；

Keywords:cDNA-AFLP, Brassica napus L.seeds, desiccation tolerance, RT-PCR,

摘 要 ：选取甘蓝型油菜种子发育和萌发过程中与脱水耐性相关的不同时间点,采用cDNA-AFLP技术通过128对引物组合分析,共获得394条特异转录衍生片段(TDFs)。经BLAST序列比对,发现其中189条与GenBank数据库中已知功能基因同源,按其功能可分为转录因子、基础代谢、抗逆胁迫、信号转导等相关蛋白。RT-PCR技术对差异显著性片段验证结果显示,泛素蛋白、油体蛋白、热激蛋白和一些转录因子片段均受到种子脱水诱导表达。研究结果表明,发生在种子发育和萌发过程中的脱水耐性反应非常复杂,涉及植物生命活动的诸多领域。

Abstract:A cDNA-AFLP approach on Brassica napus L.seeds was used to identify genes associated with desiccation tolerance during the seed development and germination.After compared with the database of NCBI,among the 394 specific transcript-derived fragments (TDFs) obtained with 128 primer pairs,189 TDFs presented significant similarity with the functional genes which can be classified into transcription factor,basic metabolism,stress resistance,signal transduction protein and so on.Using a RT-PCR approach,the significance of difference was confirmed.It indicated that Ubiquitin,Caleosin,Oleosin,HSP and some of the transcription factors can be induced by the seed dehydration.All the findings revealed that the progress of dehydration happened in seed development and germination is very complicated and involved in many fields of the plant life.

---