---

作 者 ：肖炳光  杨本超

期 刊 ：西北植物学报 2006年 6期

关键词：烤烟；反转录转座子；IRAP；聚类分析；遗传差异；

Keywords:flue-cured tobacco, retrotransposon, IRAP, cluster analysis, genetic difference,

摘 要 ：利用基于反转录转座子(IRAP)的分子标记技术分析了59份烤烟品种间遗传差异。结果表明,根据烟草反转录转座子Tnt1、Tto1、Tnd-1序列设计的5个引物共扩增出151条带,其中多态性带145条。每个引物检测到多态性带10~51条,平均为29条。供试材料间遗传相似系数变幅为0.140~0.760,平均为0.540。通过聚类分析可将参试品种分为14个类群,国内大多数推广品种、育成品种被聚在类群,说明其遗传基础比较狭窄;地方品种翠碧1号、小黄金1025、黄苗榆和净叶黄等各自聚为独立的类群,可利用它们作为杂交亲本来拓宽烤烟品种的遗传基础。

Abstract:The genetic differences of 59 flue-cured tobacco varieties were examined by retrotransposon-based IRAP markers.The results showed that 151 bands were amplified with five primers designed according to the sequences of the retrotransposons Tnt1,Tto1 and Tnd

---