Establishment of a high efficient regeneration system in vitro and transformation mediated by Agrobacterium rhizogenes in Buckwheat (Fagopyrum esculentum)
Abstract:The hypocotyls of buckwheat (Fagopyrum esculentum Moench.) seedlings were sliced and cultured on MS medium containing different phytohormones to induce callus.The frequency of callus induction reached to 100%.Calli were subcultured on MS medium supplement