免费文献传递   相关文献

Cloning and Analyzing of Gene PdZFR from Populus deltoides

美洲黑杨PdZFR基因的克隆和分析



全 文 :!!
!"#$
!"##"$"%&"(#"###)$#*+*$#8
‰Š‹Œ
,89:;
Ž1‘23
345
!
678
!
!
9:;
!
<=>
!
9?8
!
!"#$%&()#$(*
"
"+#$,#-./01234
"
56
!"###&"#
45
!
°{ApqÃ
,89:;
äj
E670
×À
"
78rOPA£FêMg>
H0O3
èxNŒOP~E670
KOPA
670"
z=ŒOPT:DÉ
*
ÀEtR¿1
*
ø¡ÉjZ
*
sft
*
luTvO¨×À>ŒOP¤wxTSTjZ
¿ƒLMNj
&
Ay¿z{x»YljYZ
$
ŒOPG¶@U/r‡
&
678
!,89:;%
ApqÃ
%H0O3%_\1$Y=?W>GB%S0S0;Mi%O00S;Mi
9:2;$
!.8&(9""
!<=>?
!0
‘’“”
$ (##$#*$"+!
|†“”
$(#"#$#*$"&
O•–v
$
?L"}‚L#$%&9:
(&%(
~ç0Ėv

Ãâ
H70>
Tn‰
*
Qgr‡OPèxK]{(
(!(##*$%$O#"#
>"
+
&8+
–v

b¨ôjÃâT1B/KGjɑJ
(!7M9(##&O;""&"#8#
žŸ 
$
ÐÄÆ
!"&8)#"
£
"
€S‚ni
"
®§
"
(fÐ
$
#-OP%4
9
!©ªž
<+/).): *)@&)*+,N.): /012)2!"#$%07/K!&()(*"+),&-"+*
<"=>.0?0$"@AB$0)?(0"9C"=>D$%&E("CA"=>#$%F(%"9C"=>B$0%&?(0
!HAIA=JEK -GIL>LCLAMF,MJAILJN" OK>GAIA0E=PAGB
=GP OC?L>T=L>MG".L=LA,MJAILJN0P<>G>ILJ=L>MG";A>U>GB!"###&"" OK>G=#
&=-37*43$SKAFC??AGBLK E670=GP BAGM<>E670MF,89:;VAJAE?MGAP FJM<,)G(/(183/5)$831RNH0O3=GP _>I$
C=?\AGMRJ=JNY=?W>GBTA?N" =EEMJP>GBLM>LI@=JL>=?E670IAXCAGEA95A=GVK>?A" LKABAGA
@JM@L>MG >G>L>=L>MG" >GLJMG =GP AiMG P>ILJ>RCL>MG" I@>EA@=LAJG" LKA=<>GM=E>P IAXCAGEAAGEMPAP =GP
LKA?ME=L>MG MFLK>IBAGAMG EKJMIICAMJMJB=G$I@AE>F>EAi@JAII>MG =G=?NI>IIKMVAP LK=L
LK>IBAGAV=IJA?=LAP LM@?=GLPATA?M@>2, ?/7@$,89:;%,)G(/(183/5)$831%H0O3%_\1$Y=?W>GB%S0S0;Mi%O00S;Mi
!!
¡
l,$H-7\!l>GE,>GBAJH-7\#
WnƒT„…
R†‡¤iMH¨@!
"
zÓYÕTYlQR
&
´
r3‡
!"20H$8)G1$15?0/$0%0 19#
OPA±VT
() %&
FOP
"
Ì
+8
FOP¡
l,$H-7Wnƒ
"
8
"9%(:&

*
v‰>ŠþOPA!
"
{‹V¡
l,$H-7WnƒTOP8
#98#: `#98):
+",
&
¡
l,$H-7WnƒT„…¤OPÀEYl
*
šŒ4×
gŽ
*
„…‘’Awfot0³8
&
˜
e“”r‡OP
"
­
D;I""*IH"*D=*"*,!J*G)+
23&(/05)2!.!(
•¡Ì
l,$H-7Wnƒ
+( ]+,
"

OP²³G“”W>ºGb҃°Ì‡
&
XB
(4
"
˜ˆ¡
l,$H-7WnƒT„…ÓÌ
3+
ú
Ë()&Vg
"
ºGúËO#–—
"
z¤úËO#
–—!t…‡˜g8
&
úËO#–—àšŒø™
š{›T„…œÎ–—
"
´„…QRSOPT
Yl
&
úË()&
3+
Gv/„…WB
"
?°v/
„…Lëú˄…&_BT
"
?°„…TœÎ
+% ]),
&
r3‡!¡
l,$H-7WnƒTOP
CK@"
º
GžŸ ¡°RS
+*,
& f=GB
w
+8,
¢2¾|£¤
!"2536$1$0 831325)2(6I@JAGB#
T
l,$H-7OP{’
¥wÕÀOP¦§T¨©g
&
¶@!=¡
9:;*=>
WnƒOPT(ªiê«E
"
=Ãâ
3.SI
u{
FC;LM¬­YZ
"
‡¤G*t¶@r3‡¡
l,$
H-7WnƒOPslT
E670

"
ÕÓT]{
#!
$
!
%
!
&
!

!
( =
(+
>
foTC;
&
tvüºe
"
¯‹Ì=Ãâ¡
l,$H-7WnƒT„…OPLMèx
*
OPWn>
YljwfoT(
&
´°D(WX]4
"
¡
O+/O%$l>GEF>GBAJ
H-7WnƒTOP
!G89:;#
YlGApqÃ
!,)G(
/(183/5)$831;=JLJ9#
²ÅqsÕ]ª-Q†]TjZ

"
cr‡ˆuº
#c&"
©/V×\]NŒOPT
äj
E670
×À
!
WX¯À4
#%
Í(¤èO¹S
"
78
H0O3
fg
!H=@>P 0<@?>F>E=L>MG MFE6703GP"
E670
±>abèx
#
èxc~E670
×À
"
78
_\1$Y=?W>GB!_>IC=?\AGMRJ=JNY=?W>GB"
rOP
AwxTê²
#
fg´OPASèxŒOP~\×
À
&
=ŒOP×ÀLM[Yj
"
(cOPWn
*
ÀENTsftHK¤OPASTjZ
%
.8
HS$
2OH
jŒOP¤qsAy¿z{!TYl
&
"!
@A()*+,
A9AB
@ABC
Œ„“1P¤!"#$%&()ø
"8
€!
³
"
èî-Q¯p´-jäVDkµ
&
<<âŨ
\¶·©lTApqÃpãþâ
"
8¸É¹«þâ
º—ªâÞ
"
»¼op½>¯p´-jäAy
"

ß¿ÀŒop½HøT¯p´-jäAy
"
i–—
!bÁN
]8# ^
^G¯Ý‡
"
èQÏ8iŒOP
H0O3
~\èx
%
sîDŒApqÃpãþâT½
Á
" ](# ^
^G¯Á‡
"
8i
_\1$Y=?W>GB
P3
&
7®½D
*
½ñ>ðñ
"
–—bÁN
]8# ^
^G¯
݇
"
8i
HS$2OH
Âæ›Ylj
&
A9CB
@ADE
"9(9"!
345678
!
9:
!
;:<;=!
H70
>
?
!
9
H70
wŒ7856ÃÄ®¨@9:ÌÅÆ
Ç"#¨éT¶@9
H70
wŒP$…
!O=Lm$ 01$
;H3$##%$"###"
ÓT%/4ÈɺNc¶ZÊ
&
9
H70
wŒN
"
8
H7=IA$FJAA67=IA!2JMS
Æ
#
ªu
") <>G"
¹º
b
ÐÑw¹«
67=IA"
£¤ËÌ
†‘
H70&
8
S03
Í[ÎOPHIUV
H70

&
"9(9(!H0O3
@A
,89:;
BC
E670
DE
!H0O3
ÓTêϺ»
O?MGLAEK
"#¨éT
.50HSH0O3
E6700<@?>F>E=L>MG Q>L
¶ZÊ
"
Н‘D
&
Œ
(
"
B
¯p´-jäAy
H70
8i=˜ÑRÀE
& +.
H0O3
*+RÀE8
[?>BMPS" ! ).$S()_7$+.#
?
@
%).H0O3
*+RÀE8
S.2!).$00\O0\S\\S0
SO00O\O0\0\S0O\O\\\$+.
>
[?>BMPS( ! ) .$
00\O0\S\\S0SO00O\O0\0\S0O!S# +#_7$+.#
?@
"
?@h\Sƨ%Bp
".C@AJ.EJ>@L-
RÀE
&Òi
-GT>LJMBAG
"#
& +.H0O3
>
).H0O3
67x
»?@j5º
+.$H=EA$l,$H7\!).$SS0O\\O0O\$
\00O00\0\\SSO00O$+.#").$H=EA$l,$H7\!).$
0\\SOOSOO00SOS\O000SOOO0O$+.#%
©8­B?
@
e25
º
!).$OS00S0O\0OSO0OS0S0\\\O00\$
O0\S\\S0SO00O\O0\0\S$+."#9%
"
]"
%
) .$OS00S0O\0OSO0OS0S0\\\O$+ ." (
"
1
]"
#
h\Sƨ%Bp
& 2OH
67Tˆ>^Gº
N¶ZÊ
&
2OH
RS}pN
"
Œ
+
"
1
8iHIUV
"%8
"
1
8iP†‘vT^b
"
P†‘P$…7856
ÃÄ®¨@ÌÅÆÇ"#¨éTÍ[ÎOPK
2OH
é@3ìP$…
!O=Lm$ 01;\H$##+$"###"
†‘NT
x»^b()t
S
T
!2JM
#"
zÀìÄ
ÓÔÕ
6/)
(ÕZ
"
8
5"+,
>
5"+H
V×?@
2OH
;<0Aèx
"
Ö"#LMV×
&
"9(9+!,89:;
FGHBC@A
"9(9+9"!
OPA
670

!
ŒApqýÁ
) B"
–—!™p›±õ
"
j
(
w1•ët¡
()<1670
wŒª«–
!"## <]"
SJ>I$/O?@/9#""9)
]"
7=O?"(: OS0;"()<]"
36S07=(""
]"
×Ë
"(#<]"
6SS#
T
)# <1
žŸ 
ø
"
¬bØÙÚÛ­®
"*) ^
´³
+# <>G"
j58
(#
<1
¹º
b
ЭB@
*
¹ºpÑw
"
·
%
OPA
670
8
)#:
£¤ËÌ
"
8Å(Ü4N¤
8#:
£¤!ÆÇ
+
·
"
MÝÅÞ
"
Íi
( <1S3&
Œ
#9) <1
OPA
670"
•
)
"
1"# ]"
67=IA$FJAAH7=IA"
­®
+8 ^
´³
" K%
•
"#
"
1"# ]"
„…&
Q"
­
®
"%) ^
´³
" K%
j58
#9) <1
¹º
b
ЭB@
*
¹ºpÑw
"
·
%
OPA
670
8
)#:
£¤ËÌ
"
8
Å(Ü4N¤
8#:
£¤!ÆÇ
+
·
"
MÝÅÞ
"
Íi
(##
"
1S3"
LMæ›>UV}ßg
&
"9(9+9(!_>IC=?\AGM<>E1>RJ=JN

!

)
"
B
!
à
)
"
1#
OPA
670
j58
-7);_*.20 -*,L( -
>
@1G-
&/0
%
&é@j5/
.@>G$OM?C!O?MGLAEK"
A"
#
jžá
&
8ÕQ£¤Ë̆‘&
NTOPA
670"
mMÝÅÞ
"
Íi
"#
"
1
âÕQ
&
Œ
%
F
(##
"
112OH
RS 
"
j5/0
-7);_*.20
#
*
,L(
>
@1G
#
"
Р!•ë=SOPA&é@
"
"
1%
j5•
"
"
1"# d1>B=IA;CFAJ""
"
10S2"(
"
10P=@$
LMJ! ) .$\S00S0O\0OSO0OS0S0\\\O0O\O\S\$
+*
=)
” ÐÄÆw
$
ApqÃ
,89:;
OPTèx>j
\SO\0O\\OOO\\\OS\\S$+.%+.$/(7$OOO\0OO0$
2[%$)."
Sƨ%
#
>
()# eS% 6701>B=IA!S0Q0$
H0"
Ä(
#"
8
PP/
(
[
ã·T¸˜
"#
"
1"
­®
""* ^
()/0
%*) ^
´³
"# <>G
âV
S% 6701>B=IA"
•
&#
"
1S3
­®
"
i
](# ^
݇
&
"9(9+9+!,89:;\AGMGB!
Œ

F
(##
"
1
2OH
RS 
"
j5/0
+.-7);_*+..20
#
*+.,L(

*+.
@1G
#
%).-7);_*)..20
#
*).,L(
>
).@1G
#
%
´S-
-7);
_*@1G
#
*,(L
>
.20
#r£F!j5Œ
"
"
1
•ëtS-
=S
(##
"
12OH
RS !
%
•
%&
"
1
67­B–
!"
"
1+.
¿
).$H=EA$l,$H7\"#
"
<*"
"
102).$\S0$
0S0O\0OSO0OS0S0\\\O$+. "#
"
<""
"
1P7S2"#
<]"
")
"
1"# d3iS=X ;CFAJ""9) e3iS=X#"
­
®NŠœLM­¯
2OH
67
$&* ^
±5g
" <>G%&)
^ "# I"* ^ * <>G!
?F89•
) I#"+(
F89
%8(
^ "# <>G&
67}ä
"
Œ
+
"
1HOH
é@LMHIU
V
&
<).
>>
+.
>67DEX\Té@
"
P†‘vT^b
&
LM
S
Tèx
"
8
5"+,
>
5"+H
V×?@78
2OH
fg;<0Aèx
"
z=î
gèxLMV×
&
"9(9%!HS$2OH
IJKLFGMN$%
!
pŒ½D
*
¯p´-jä
*
ðñ>½ñ9
H70(
"
B"
j58
[?>BM
P !S#()
>
!!JM
RÀE&LMRÀE
"
RÀE^G
>TˆºN
H0O3
=˜Ñ
E670
Bp
&
=˜ÑBp
}ä•
&#
"
1S3!@/9##"
­®
%

)
"
1
RÀEé
@
"
H
AD#
OP
!).$\SS\0SSSSS\OS\\\00\O$
+.b).$\0SOSS\\OOSSO0O\SS\S$+.#
=N
"
HQ
åg=N
"

G89:;+. H0O3
é@Tjæî
g=N
"
8
).$H=EA$l,$H7>
+.$H=EA$l,$H7?
@LM67
"
RSTˆ
)#
"
1$
qsAy
E670
=˜Ñ*+
$)
"
1%
).$H=EA$l,$H7?@
!"#
"
]"
#$"
"
1%
+.$H=EA$l,$H7?@
!"#
"
]"
#$"
"
1%
P7S2!"# <]"
#$"
"
1%
"# d2OH;CFAJ$)
"
1%
JS=X!S=Q=Q="
Ä(
#$#9()
"
1%
Q
$
˜
)#
"
1
674×
$&* ^
±5g
" <>G%&) ^ "# I"*% ^
"# I"8( ^ +# I"+(
F89
%8( ^ "# <>G&
67}äŒ

"
1
LMHIj
&
"9(9)!
)*$%
!;?=IL
j%Ó>H¨u{F
!GJb
GL#
7O;-
çèT
,S2
¯
&
[=4×.8
;?=IL
F
G!T
;?=IL=?

"
Vg78
;?=ILG
>
;?=ILi&
H
¨u{F
GL
>Ué`u{F
GJ
mÎêt±ëæ¿
ƒ
"
Wp͓u{F
&
Ãâ~OPAu{F´ìX
Ã
!,452$7?)702G0 SMJ9n\J=N#
~OPAu{F¯
t͓±ë
"
mÎêNWp͓ÃâOPAu{
F
"
8iOP¤OPASæ¿j
&
(!
-.*/0
CJAB!"#$%
Ž’“
45O&

´
"
_He4
$
9
H70
HI!
(.b".
^bí][ÙÄi
("
YZwŒT9
H70
m
H7=IA$
FJAA67=IA
ªuN}ßgYÚ
"
՜Î>LjîwV
•
&

(*+
º
,89:;
OPT
).H0O3
>
+.H0O3
6
7
&
´
(
e4
$,89:;).H0O3
èxx»gY
Ú
"
67^bY˜
"
Äcº
&## R@
ï_
&
´
+
e4
$
OP
+.H0O3
¤
#9)# WR
ï_674x»^b
"
¤
#98) WR
G
"9## WR
ª674Ux»g^b
"
a_{i?@Ux»g67?t
&
©/P†‘
).H0O3
é@>
+.H0O3
é@
)## R@
ï_ªx»^b
"
èxÀ
ìÄÓÔÕðñfšŒ
"
mV×j
"
¹ò0’×À
"
\]
,89:;
OP
" #"% R@
T~\×À
!

%#&
.8
670507
FG=ŒOPLMj
&
ŒOP~70
×À¡
8) R@
T
).eSH" *" R@
}ßlu×À
>
(&+ R@
T
+.eSH%
c!
"*" R@
lu×Àlu
"
^
(#)
FvO¨ApTeóÑ
"
cwH1º
*9&%"
±
VTjɛº
(+9% W6&

)
º
,89:;
OPGr3
‡
I+CI%5EG3;*=>N$%&32
OPvO¨[=‰Š
"

YV4ÔÕTslg
"
l
*:&
!!!!

"!H0O3
23T9
H70
HI
!!

(!,89:;).H0O3
HI
!!!!!!

+!,89:;+.H0O3
HI
!!!!!
&+*
#!
$
!
%
!
&
!

!
( =
(+
>

%!,89:;
OP~E670
×À
!
̯œväjºôõluö
#

)!,89:;
OPGr3‡
I+CI%5EG3;*=>N$%&32
[=WX
CJCB!"#$%
ŽO’“
(9(9"!,89:;
OPQFGHR@A
!

*
YZ
$
).$H=EA$l,$H7\@J>¤
-7);_
r£F!èx
TDEX"
l
)9# WR
HS
%
¤
.20
#r£F!6
7é@\]º
(9) WR%
¤
,(L
r£F!èx4
(
^x»D®
"
j5º
"9)*"9# WR
ï_
& +.$H=EA$
l,$H7\@J>¤
@1G
#r£F!èxTDEXÄ
l
(9# WR
ï_
%
¤
.20
#r£F!èxD®º
(9# WR%
¤
-7);_
r£F!67D®Äcº
%## R@
ï_
"

,(L
r£F!èxD®º
"9& WR&

P†‘
).$H=EA$l,$H7\@J>>
+.$H=EA$l,$
H7\@J>67é@!D®\]X\T^b
"
()
S
T
"
ÀìÄÓÔÕðñfšŒ
"
÷ŒYèx
"
m
V×j
"
¹ò0’×À
"
XN\]\]º
)9%+ WR
TDEu{
&
(9(9(!,89:;
FGSTUVWXY
!
.8
.@>PAN
FGj
,89:;
OPT’É>ø¡ÉjZ
&
Œ
"95=JWAJ%(9).B$.20
#
%+9).B$-7)2_%%9).B$,(L

%)9).B$@1G
#
%
*9+.B$.20
#
%89+.B$-7)2_%9+.B$,(L

%&9+.B$@1G
#

*!,89:;
OP¤OPAr£F!67THI
OP"#
F’É>
&
Fø¡ÉAp
!

8#"
c
!
"
’É
"#
D®XÄ
"
º
%#+ R@%
’É
&
·Ê
"
#%*
=)
” ÐÄÆw
$
ApqÃ
,89:;
OPTèx>j
º
""( R@"
c`D®\]•ci
"## R@"
XcTh
++
R@& &
Fø¡É!
"
Ì

FÄi
"## R@"
X\l
+(
R@&
´ø¡É>’ÉÄcjZ_He4
$
ŒOP
s@W[Y_L
& "WR
ï_TOP¡
&
Fø¡
É>
"#
F’É
"
b’ÉTRh\]R†Yø¡
Éc
&
ŒOPø¡É
).
>s¿ƒøOº
0!e#!e#
#
\e"+.
>s¿ƒøOº
0#
0!\#e!0#&
´sft_H]4
"
ø¡Ésù5¿ƒW>¯
iÝú
"
º
10\.
>
1\e."
ûB
\e$0K~
"
»i
˜üH
mø¡Ésft
!

#&
ø¡ÉG
’ÉDEÄcjZ‹Y
"&

8!,89:;
ø¡É>’ÉjWX
!
qxOYø¡É
"
ýxOY’É
#

!2Pl,H
OPs¿ƒ
(9(9+!,89:;
FGOZ[\]$^
!
78
;?=IL
F
G!T
;?=IL=?

"
|wxTSèxT~\×À
=͓ÃâOPAu{FLM[=j
"
UV
,89
:;
OP¿iÃâOPA
1\j_-
S
"
c!
"%8$%#)
=Si
1\j_-
S@u¿ƒT
"**"**(+$"**"()*%
%"#+$%8%+
=Si
1\j_-
S@u¿ƒT
"**"()88$
"**""&+)%%8*#$)+%)
=Si
1\j_-
S@u¿ƒT
"**""&"&$"**""++("
!"T݊×À_{GÃâO
PAþ)/4!Tÿ[̇
&
ŒOP¤ÃâOPA
1\jo_--
u{!SÌ
((%
FøOTsl×À
&
F
AB!"#$%
Ž”•–‘i—–2˜™š
’É
b
F
OPADE
=S¿ƒ
=
S¿ƒ
’É
\]
bWR
˜$g
b:
ø¡É
b
F
ø¡É
\]
bWR
" ""8 ]")) " ]+& +& "##9# " "("
( "&8) ](#+% %# ]&& *# "##9# ( ""(
+ ("%) ]("88 "## ]"+( ++ "##9# + +(
% ()#% ]()" "++ ]("# 8 "##9# % "&8
) (88 ]((" ("" ]()% %% "##9# ) +()
* +"%) ]+") ()) ](&) %" "##9# * "(+
8 ++#8 ]++& (&* ]+8 + "##9# 8 "(%
+)"( ]+*#+ +8& ]%8# &( "##9# &
& +8## ]+"" %8" ])( ""( &9( & "()
"# +&+8 ]%++& )+ ]&) %#+ &&9)

&!,89:;
OPÀE:R>"e¿ƒ
(9(9%!,89:;
ST_`!

&
º
,89:;
~
E670
×ÀGOPASèxTOP~\×À[=
TäjWX
&
´
&
_He4
$,89:;
OPÀEt
RøOº
\! 4""" "8#"
´
4"
¿ƒÊüÕZ’T
MH¨@:DÉHŸ×À
S0S0
ö
"
´
4" !" "8#
t
4"+!" +##

0bS
¡›Z’YÕ
"
_{=OP
"%*
#!
$
!
%
!
&
!

!
( =
(+
>
TYlt#8
%
b¤ÀE"eTøOG:Rr

\"
¤ÀE"e¿ƒª‡¤($T
0
¿
0_
×À
"
Ì.i¤ÀE/4!op,ä)%Wn
"
ÌO
PÀE"e
&
.8
210O3
FG=OPAS\]T
,89:;
OP~)9%+ WR
×ÀLM:DÉj
&
j
WX¬YZ
$ ]"## R@
ö÷øÕ&oMH¨@:
DÉ@G
S0S0
ö>
OO00S
ö
&
(9(9)!,89:;
FGOcHdReMN
!

"#
!#
º
9:;=>
OP¤qsz{¿Ay!TYl‰Š
%
ºøº
AD#
OPT67^bHI
%
%ºpA
ywŒT9
H70
HI
&
´
"#
_He4
$
,89:;
OP¤ðñ!Yl›XÕ
"
¯p´-jäA
y·Ê
"
½DAy>½ñ!Yl›r[Õ³
&
ŒO
PTYl¤Ãâ¯p´-jä>ðñ!Yl›*
Õ
"
b¤½ñ>ðñ"³¥Ì[»
&
\i23Œ

8
€1
"
bèî¬|à¤-Qop>ðñAy
U/Tkµî”
"
‹VŒOPGU/r‡
&
"95=JWAJ%(9
îg=N
%+9
åg=N
%9
¯p´-jä
%)9
½
%*9
ðñ
%89
½ñ

"#!,89:;
OP¤qsAy!TYl5ì
+!
-1*21
Í£.8
H0O3
fg=ApqÃ
,89:;
OP
LMNèx
"
z(.8rwxTêM´OPASp
]èxNŒOP¡:DÉ>ÀE"ebÒ¤øT
~\OPD®
& H0O3
èxfg*e
"
Í(78
55$1_
RÀE&±>À²&>*+ÀJ]{¤R
ÀE/4!l)?ë),?@
+ ]&,
&
ŒfgGc)
fgr[~*N
¹+¨OÄ
*
¹,ÉWn>
-1H.¨),()w/0%êÏ
+"# ]"%,
"
œ^N
%?tT
H70
œÎ12
"
wÕNèx
E670).
>}ß×ÀT3k
&
¤h4äj
E670
×ÀO¹Ê
S
"
78rOPA£FwxTêMg´OPASè
xNŒOPT~\×À
&
OPA
670
mù5
%
F
øOTR>ø&ªu
"
CNW>()i%BpT
),
"
WprOPA£F
&
78),×À>x»
?@×À67OPASh4×ÀW5¯4ƒ
&
)
,(
^\6q˜T7ã-189H.¨m:;o
p
"
c!6T-189H.¨
).
>LM+¨ì|
<
"
̗GOPA
670+.
>
$[/
()
%+.
LMvO
ì|<
"
=>¤
2OH
67/4!
670
1B&T6
78
&
¤
2OH
?@PaS
"
6),×À
+.
>LM
vOì|<
"
z(G\),×À
+.

"
Ý¢OP
x»?@67t),±>N
"
),?@TWB¿ƒ
rop
"
´b7•
2OH
67Tx»g
&
ŒfgG£
?
+"%,
 ƒTwxTêMO*r[ÓÌx»gÕ
*
%ŸY
*
¬b
*
0_gÚwx1
&
¤h4äj×ÀTO¹S
"
=ŒOP¤Apq
ÃOPAST×ÀGÃâOPA×ÀLM[=j

"
YZŒOP¿iìXÃOPAu{!
1\j_-
S
"**"**(+$"**"()*!)"&(b)+(+"&8:
sl
#%
¤
1\j
o_--
u{!Ìäjsl×À
! ((%b()8" 8:
s
l
#"
¤c‹wxT¿ƒ@Aqtsl×À
&
a˜W
XYZ
"
ŒOPYl1\j_-
ÀEb¾
&
´[=W
X¾e
"
ŒOP¤ÃâOPA=S×ÀSÌ
+
F¿
ƒ[=quB
"
u{F!Tu{²³YVºŠ‹V
•
&
Í£]tTŒOPwxTS×À\Apqé
/
\AGMGB
l)\]
"
u{[Y_`
%

â~OPAu{XN©/ěV×HÉþ)bp
&
\èe¾
"
CCÃâ~OPAu{hm\]
"
û¦›
=cu{ªué¨Tÿ[LM||
"
)cà¤($
³FrsøO4V¿ƒ
&
,89:;
OPvO¨×ÀGr3‡
I+CI%$5EG3
;*=>
OPTvO¨×ÀÌ
*:
Tslg
&
ŒOP
Ó
%# *`#
FvO¨ApTDWnƒ
"
¡[s×
À
O$o($O$o!&$+&#$O$o!"$+# $/$o!($+#$O$o($O$o
!%$%#$O$o($O"
ŒWnƒ_WB
(
FDžÉ
& HS$
2OH
UVWX’
"
ŒOP¤¯p´-jäAy>
ðñAy!Yl›YÕ
"
)cà¤ðñ!
"
b¤p´
½D>½ñ
!
p^Eñ
#
Ay!Yl›^
&
\iÃ
âð׺FGð×
"
ð…¨iFGð×T9HS
"
F
Gð×T9Ht…Gâ-²ÅrsTIx
"
Pè¤
ðñU/kµ«EŒOPYlY›Õ
&
»Jt
,89:;
º¡DWnƒT„…
"
bD„…¤OP
ÀE
*
„…"r78
*
Âb҃°>OP„…Q
Rwfott0³8
+") ]"8,
&
Pè‹V
"
ŒO
PGÃâ·¨¨\r‡
%
ûŽivü¡
I+CI%$
5EG3;*=>
WnƒTOP]{(KX[Y+
"
¬Õ
ÓT]{foT£?
"
PèŒOPTÓT]{
>Gcr78T„…¦Ì§i©/c)23q
EL˜ê¢2
&
(%*
=)
” ÐÄÆw
$
ApqÃ
,89:;
OPTèx>j
Z[!<
!
+", 2ALAJQ" Q?=CI,o5" OKJ>IL>=G . /93T=?C=L>MG =GP E?=II>F>E=L>MG
MFH-7\$F>GBAJPM<=>GIAGEMPAP RNLKA0J=R>PM@I>IBAGMGMM?MBN" (##(" +!%#$"( ]("
+(, ,JAAGBAJ$ =GMTA?@JMLA>G IAXCAGEAFJA$
?=LAP LMLKAk>GEF>GBAJ+Z,90GG 7f0E=P .E>""&&+"*$%"8%
L
%"&(
++, Z=EWIMG 2Q" 3?PJ>PBA0\" ,JAAP 3"350/4SKA?MJAMFLKAH-7\I$
ICRILJ=LAJAEMBG>L>MG=GP E=L=?NI>IRNCR>XC>L>G ?>B=IAI+Z,9SJAGPI
OA?;>M?"(###" "#$%(& ]%+&
+%, ,JAAXC>L>G=L>MG$ H-7\FMJPAILJCEL>MG+Z,9OCJ;>M?"
(###""#$H% ]H8
+), 1MJ>EW Q1" ZAGIAG Z2" ,=GB.9H-7\F>GBAJI=LACR>XC>L>G$
EMGUCB=L>GBAGkNXC>L>G=L>MG +Z,92JME7=L?
0E=P .E>e.0""&&&"&*$""+*% ]""+*&
+*, 6MGBO/" 0B=JV=?5" lK=GBff"350/9SKAGAB=L>TAJABC?=LMJMF
@?=GLEM?P JAI@MGIAI" /[."">I=H-7\3+ ?>B=IALK=L=LAILKA
CR>XC>L>G=L>MG=GP PABJ=P=L>MG MF-O3" +Z,92=GI"(##*" "#+ !("#$
(" ](*
+8, f=GBo" .CG O" /C f"350/95M?AEC?=JE?MG>GB=GP EK=J=ELAJ>k=L>MG
MF=BAGAAGEMP>GBH-7\k>GEF>GBAJ=GWNJ>G @JMLA>G FJMLM?AJ=GL0JLA<>I>=PAIAJLMJC<+Z, Z;>MIE>" (##" ++$"#+
L
""(
+, .EKJ=<<\" ;JCEKK=CI-" HMAPAJS90I><@?A=GP JA?>=R?A).$
H0O3=@@JM=EK+Z,97CE?A>E0E>PIHAI" (###" ($ A&*
+&, O=J?Y6"\=RJ>A?=.96TAWI?AJ2OH2J>+5,97AVNMJW$OM?P .@J>GB/=JRMJ1=RMJ=LMJN2JAII" (##+" +)& ]
+&
+"#, ;AJL?>GBY 5" ;A>AJ," HA>EKAGRAJBAJ396ALAJ<>G=L>MG MF).
AGPIMFI@AE>F>EB=IA$PA@AGPAGL=<@?>F>E=L>MG
+Z,92OH5ALKMPI0@@?""&&+" +$&) ]&&
+"", ,JMELALH90K>BK?NIAGI>L>TA<=@@>GBLKA) . LAJ<>G>MFE0E>PIHAI"
"&&+" ("$ "*+ ]"*%
+"(, 1>C o" \MJMTIWN5095=@@>GBLKA). =GP +. AGPIMFLALJ=KN$
?=GBH70?>B=IA=LAP =<@?>F>E=L>MG
MFE670AGPI!H15$H0O3#+Z,97CE?A>E0E>PIHAI" "&&+" ("$
%&)% ]%&*#
+"+, .=?>AH9OK=J=ELAJ>k=L>MG MFLKAAiLJARNH70?>B=L>MG$2OH+Z,92OH+"%,
É
!
®
"
é
!
¾
"
MNO
"
w
9
S8iwxTê²T
2OH
679
:T(L&
+Z,9
‚ƒ
"(##*"( !)#$)8 ])&)
+"), 2AGB/";ABB\3" .EKC?Lk6O9HAEMGIL>LCL>MG MFLKAQH0;$Q02$
" JA@JAIIMJEM<@?Ai$ =G>GBLKAM?AP$EM>?PM<=>G$=LAP@JMLA>G$@JMLA>G >G$
LAJ=EL>MGI+Z,9Z5M?;>M?"(###" (&)$""+& ]""*(
+"*, QAGLI>I0"6VNAJ3O"2AJAkZ5"350/9SKAH-7\PM<=>GIMFLKA
@JME?ACWA<>=@JMLA>G 251=GP LKA=JAG=T>J=?@JMLA>G lJA$
@JAIILJ=GI?=L>MGRNP>JAEL?N>GK>R>L>GBLJ=GI?=L>MG >G>L>=L>MG F=ELMJ
A-,%3+Z,9Z5M?;>M?"(##"" +"($*#& ]*(+
+"8, ;MJPAG Q19H-7\PM<=>GI$ <=ILAJRC>?PAJIMF+Z,9Z5M?;>M?" (###" (&)$""#+ ]"""(
+%*