以福建白砂林场马尾松实生种子园自由授粉子代为研究对象,从215对微卫星(SSR)引物中筛选出6对多态性比较理想者作为分子标记对种子园进行交配系统分析。结果表明:1)该种子园的异交率较高,多位点异交率2006和2007年分别为1.200(标准差SD=0.000)和1.072(SD=0.066); 年度间异交率相似。2)不同冠层和方位上的异交率大小为:上层>中层>下层; 东面<南面=西面=北面,与其花量的调查结果相吻合。3)从坡向来看,种子园西坡与北坡的异交率无显著差异。
A mating system of the seedling seed orchard of Pinus massoniana in Baisha Forest Farm,Fujian Province, was studied by microsatellite (SSR) markers. Six SSR primers selected from 215 primers were used for the mating system analysis. The results were as follows: 1) The outcrossing rate of the seed orchard was very high. The multilocus outcrossing rate was similar between 2006 and 2007, and was respectively 1.200(standard deviation, SD=0.000) and 1.072 (SD=0.066). 2)The outcrossing rate in the top canopy of the crown was higher than those from the middle and bottom position of the crown. Eastside of the crown had a lower outcrossing rate than the other sides. 3) There was not significant difference in the north and west hillside in the seed orchard.
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