选取颐和园不同树龄油松及其衰弱株的一年生针叶为试材,测量不同季节的针叶单位质量的长度、叶绿素含量、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性等指标。结果表明: 与健康油松比较,衰弱油松树冠不完整,一年生针叶柔弱细长,针叶单位质量的长度值大; 衰弱油松叶绿素含量低、光合能力弱,叶绿素a/b值低,光能利用效率低。在一年的生长季节中,油松MDA含量随针叶的发育逐渐升高; 与健康油松比较,衰弱油松针叶MDA含量远高于健康油松,而针叶SOD总活性和SOD比活力均低于健康油松,其抗氧化能力弱。这些结果说明: 针叶单位质量的长度、叶绿素含量、MDA含量、SOD活性等可以作为古油松衰弱衰老的诊断指标。
This research investigated the physiological and biochemical diagnosis indices of the weak and senescent ancient pines, Pinus tabulaeformis which were expected to provide scientific basis for their rejuvenation and management. The annual pine needles were used as materials from healthy or weak pines with different ages in the Summer Palace in Beijing. The ratio of mass to length, chlorophyll content, MDA content, SOD activitiy of pine needles were measured. The results showed that, comparing with healthy pine, the senescing and weak one had irregular crown, soft and slim needles with higher ratio of mass to length, lower chlorophyll content and ratio of chlorophyll a/b. The MDA content increased with needle development during growth period, and was higher MDA content in senescing and weak ancient pines, The senescing pine had lower total SOD activitiy and SOD specific activitiy. It was summarized that the ratio of mass to length, chlorophyll content, MDA content, SOD activitiy of pine needles could be used as indices to diagnose the senescence and weakness of ancient pines.Finally it was discussed that these physiological indices were used to indicate the growth status of ancient pines.
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