The effect of culture media,temperature and humidity on the mycelium growth, spores germination of Metarhizium MS01 strain, a high virulent strain from the soil and the pathogenicity against Anoplophora glabripennis larvae was tested. The result showed that the optimum culture medium was PPDA, the optimum temperature for the mycelium growth and spore germination was 26 ℃, and A. glabripennis larvae had the highest mortality from the fungus at this temperature. The optimum RH for the mycelium growth ranged from 95%to 100%. The speed and rate of spore germination increased with increasing of RH. The larvae died from the fugus most quickly and the mortality reached the highest under 100% RH.
全 文 :! !"" ! ## R TTH,*NB2 /3NCP N ?@2Q MO/P #"W N/ 5%%W 4 +B2 R322Q >?Q O>N2 /M R3/O2 @2OPC?>NC/? C?KO2>R2Q FCNB C?KO2>RC?@ /M
$ % % #$ # %
& ’ ( )
&’()*+(, &(-.,) &(*(’,)
./01!"**/1#
&234*
!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
$ % % #
/MN !"#$NRKhCj:ScwbcOPXo
V’QR6:n(KSTm"
#7%5Q$ 8^’5 m9:5 # ;5 {<=5 EO95
A51 p/z’[)&)F M0 %65%%%X$1 }Q[)s,()DEO $% 75%%$#c
$%&q (;H 8&%5H‘XH7)80XH":^Te,XFxVX‘+*
’()*+#&69717%5 ,-./0#, ,12+#5%%5 : 6!;;3$%%#4%# : %5"; : %"
def#q$%%; : %9 : $"l
ghijqp/z’[)(lS<_tA,<%!%$%5cl
" L[ew~!"#l
%&’(’)&*+( ,-+.+*/0.&1/&*1 ’2 !"#$%&’(’)* +34 0220*/1 ’2 /0560.+/7.0 +34 -75&4&/8 ’3 /-0
6+/-’)03&*&/8 +)+&31/ +,-./-.&-%$ 0/$1%’.",,’2 (+.9+0
=>?@ A>/B
A51 !"#$%#& ’"(()*) "+ ,)-). /*#.01(%1#2( 34.5)#$.%& 62"7.4* %65%%%X$1 84$%.%1%) "+ 84$)0% 90.)40)$ "+ :;)<.24* 34.5)#$.%& ,24*=;"1 75%%$#c
:;1/.+*/q +B2 2MM2KN /M K<0N
8&%5 RNO>C?*> BC@B SCO<02?N RNO>C? MO/P NB2 R/C0 >?Q NB2 3>NB/@2?CKCNL >@>C?RN /4"@("@;"#2 *(2-#.@)44.$ 0>OS>2 F>R N2RN2Q4 +B2
O2R<0N RB/F2Q NB>N NB2 /3NCP
@2OPC?>NC/? F>R $9 U*>?Q / 4 *(2-#.@)44.$ 0>OS>2 B>Q NB2 BC@B2RN P/ON>0CNL MO/P NB2 M@
VG4 +B2 0>OS>2 QC2Q MO/P NB2 M<@
H":^TA/4"@("@;"#2 *(2-#.@)44.$c$ECR
%:3B0zT;,*-V2<[*HI*wx=z
:R[#>AO:K2*$%%7X=@T*5##$c*y#Y
,*ECneB[9:3*7*PB*lH":^
TA3Xd2*5##6XO/2*5###XO0~2*
$%%7c*|\H":^T2;,:Z=H7*lac
V!3lM(;HA>)%2#;.=.1?c$zOP:x80
,HAH*jo(;H-x.PB34z&;,:
*l*8Id>,*h?_*zH2;,DE4"A
[|W]eAUdA2*5##"Xiao2*$%%7X9J
@2*$%%"X=)82*$%%;XOA~2*5##"X(B>O>
)% 2( 4*$%%7X&BCPCI< )% 2( 4*$%%7c*V’[p1=n*
|TBAP(;H*lH":^Ta3V{,bD
El(;H)
*^zdohr>A3Bu*5##!X?C*$%%5X
E2?YC?R )% 2( 4*5##!X,POCNB> H2 ’O//R )% 2( 4*5###c*
9‘+*1%’FBH‘*;,,IY&JKp>
23456*FV*xV$789y*: $hzR%
:JK5_l5l*drO4"B*DEj:H‘:
H7)80&9Q19:*\HexfP>H‘m
nH7*lrZR%A
‘*1%DEB!wX(g*FV*xVp>\H0
Hy*eDY&D_o5:78*Vd!wFxVp
>+$0B>H‘\H":^Te,:‘+**w
N5xfP>H‘J\IJl‘l
$ $%
H‘B&LU\H":^Te,rZAy*:(;
H 8&%5l\5s,qV>R(2,*9ew<}R
: 6%^?e,PB‘+0$0l
51$ HIR LP "4X(g@a*’>I32Yq*>*[7
7 @y-: 5*\$GT[!5 @y-: 5*8@&[!y6G$[ %1" @y-: 5*
\’0 %1" @y-: 5*]2&[!y6G$[ %1%5 @y-: 5*El 7% @y
-: 5*ZE 5" @y-: 5XTH,q-fF $%% @y-: 5*GHl
$% @y-: 5ZE 5" ^ $% @y-: 5XTTH,qTH,Ie 5% @
YzJX&H,_q5% @y-: 5*GHl !% @y-: 5*jzK
! "y#$ %*ZE %& ’ !( "y#$ %X)*+,q)-+,BPF~
lWXGHll
%./ jk? *)(%?@lmnop:; OLB.
e’(ERdeeMF $ 0( c93V9QVw
% "y#$ %*dPD4J0’X(1:(;H *)(% H
‘:D_*PN*OPrOP !( 123*HjQEB:
D_OPu[*D_jQEQVw %(0hy1#$ %l
%.4 jk? *)(% ?@5{qNK %cH0:’
(Hy P:Cw & 11:pqrdX(Sw‘<
:H0lm’q+H0.*DUZt‘X(g8*V
gB!wFV*xV*HbxB?H+X(*Pny
M
(g*FVxxV\H0Hys>:78*\HyG
Hmnj/?@*P )5))=>Ar>s20?@l
!cH0:eD9 dX(g8X( %4^:H
0*P:Cw & 11:pqrd()X(SB*S
w:%g’H.*kR.e{OLB*e (.%6 MF
$ 0(:{H+ !( 1#*
Pr56ar?@*!‘H‘:HyoV&eD<
*jN:;>H‘:0
4(!#@1:D_jQEZ(*q8*e’*qdX
(SBP{H+-/9EeMxA78 9 %((6c*gB
!wFVA%&*!(*!!*!&*!:*!0*/(*/& ;c+vFX(*
X( 0
R(rQ &h>b*Rh>b!IB 0(hD_l
4cxV\D_o5:78 APL4gxV
kEd%Gp>+cnxVl!wgxV:S\x
Vwq!S+ %((6*=!)>4 ?&6*@A)>4yB8!>
?(6*=CD 0&6*EFE>/B&6l’ 4(!#@1:D_j
QEZ(*q8*e’*qdX(SB*P-Z!w
VkE:9EeWX{H+9EMx*X(SP{
W4%{*d !0 ;vFX(l.pb_acw8l
%.& J3ros+Kjk? *)(%?@btuvK
Lw>9xy% L\R(‘
hy1#$ %:(;HD_jQEB-0 /( G|{*c
D_>7d,Oo3*SDH:e,DZT@1p
:34o)B*R( /m*w‘fo&&PMF $ 0(
{H+:’I"w\b*R’I /(c,*d!wF
V*xV+X(1p*R^.pXOH":^Te,
I?)**VUUI,lYN_[9’B,KVe
Hq:zpwbOzBl5Ug2RSL’I;,
9*I?92-‘De 5>#>2(?@,bA7HGGADD
!" #$ I*%?BBc*_9‘I‘
%((6*
/bz3:I?(*YSw9d56V2:I
?\5Umn:78*\bMI?&9I?(sB
&6:!mn/blY/bI?(x‘I‘<"p
FV*xV\>H‘‘+*:78l
! \h<)@
!.% J3HIRQros+bjk? *)(%?@z
I5HQVl9DE %c X(g\H‘’(Hy
xeD:78 S *)(%H‘d &4,HAHX(
g8X(*$09H0Hy:C*%4 ^R$0eD
9*mn:o %lNo % ^YZU**)(% H‘d
55-+X(g8H0Hyzs*%4^‘H0:Cw
AB.4% K (.%0cL1*%s2[B9d 4 4X(g8:
H0:C*>H‘d 55-+X(g89_eD‘<
^W3*eD9zA*%s2AB9d 44X(g8
:eD9*Qdb5UB^.pZ>H‘d 55-+
X(g8Hy‘H0aON*%*OY8w>X(g
\ *)(% H‘:’(HyxeDu$zZA:*5
lY+JK56\H‘’(HyxeD9:78u
KP 55-+ X(gmn$0l9m$d )*+,*
CFMNAOx 5-+X(g8**)(%H‘dX /4X(g
8:Hy9{s20r>*|d 5-+X(g8:e
D9%s2AB CFMNAOX(gl*)(%H‘z!m
Zd )-+,8Hy*H0:C%s2sB9d’I*
eD9^WIl
!cFV\H‘’(HyxeD:78 FV
\(;H *)(%H‘’(HyxeD:78mn:
o !lNoB^YZU*>5UH‘rZWn:F
VHy9:*d$5:FV9:>A%& ’ /& ;cu^
Hy*|!wFV+>H‘’(Hy9xeD)*
ps!wl(;H*)(%H‘d !: ;p>+’(H
y9z[*HyoVL‘<{W1:H0T,
A%! P (.?(c*>FV+H‘:C(z[*tpHyo
VzslNoB^YZUqlFV+eD9^WA*
9mw !0x !& ;p>+*{#~<’(Hyxe
D9r>u!s2*|s2AB /( ;p>+:Hy
9*Hyo(xeD9*%s2AB !!*%& x /& ;
p>+:Hy9*d %&x /& ;p>+*H0Hy4i
=>*<=!eD*9Hy9zd%s2r>l5UB
?&%! ?# iow2q(;H *)(%H‘:H7)80&d!wFxV+\H":^Te,:‘+*
9 ! bcUV[W"#$!NRNBhiclXik
%&’(! )*+,-. &/0 1+/202& 3*+041-2+/ +5 6-*&2/ "#$! +/ 02557*7/- 870248
X(g
!"#$%&
H0:C
’$(&")"* +, -+.+/01-&
r>s20
2$3/$,$-(/-" +, #$,,"*"/-"
45 # 6768 6764
9_eD‘<
9"3$//$/3 )$&" +,
-+/$#$( :*+#%-)$+/1;
eD9
<+/$#$(
:*+#%-)$+/146=
r>s20
2$3/$,$-(/-" +, #$,,"*"/-"
6768 6764
>>’? =754 @ 674A ( ? BC 4D=7B= @ 4D7CA ( ?
2!?E 57B6 @ 6745 F 9 B4 8A766 @ D785 F-# 9
<(G:"H 578D @ 676A F 9 D4 IA766 @ I7B8 F# 9
>’? 57CA @ 6746 F 9 =6 =B766 @ 4I7AB - 9
2’?E C7AI @ 676D - < B5 C=7CC @ 4758 # 9
9 9 bcOXoNBhi
FV
J"&:"K
*()%*"1L
H0:C
’$(&")"* +, -+.+/01-&
r>s20
2$3/$,$-(/-" +, #$,,"*"/-"
45 # 6768 6764
oV M‘<
N()" M J$&"
eD9
<+/$#$(
:*+#%-)$+/146=
r>s20
2$3/$,$-(/-" +, #$,,"*"/-"
6768 6764
IB =78A @ 6744 ( ? ! O 6788" P 676D #I O 67DDB 4=8766 @ 8Q65 ( ?
IA =75C @ 6746 (F ? ! O 678C" P 67C4 #I O 67DD= 4DB7B= @ 4=QB5 ( ?
I8 =7C4 @ 67BB (F ? ! O 678I" P 6745 #I O 67DD5 4857CC @ 4CQ=I ( ?
C6 =76B @ 6765 F ? ! O 675D" P 67I= #I O 67DDB A47CC @ 8Q=A F 9
II 57=C @ 67I5 - 9 ! O 67CI" P 67C6 #I O 67DDD 8A766 @ DQ6= F 9
48 I78D @ 676= # < ! O 674B" P 67IA #I O 67DB= 6766 - <
C8 47DI @ 676A " ’ ! O 676A" P 67=C #I O 67DD6 6766 - <
c^.pZ IB L+H0:HUQ%*Z_*OY(;
H!264H‘’(Hy:zmFVw IB L*eDzm
FVw I8 R IA L*FV@AY@su!ABeDl
CcxV\H‘’(HyxeD:78 xV
\!264H‘’(HyxeD:78mn:o Cl
NoB^YZU*S\xVw 466Sx D8Sp>+*
>H‘:Hy9*Hyo(xeD9uW[*Qd5
UB^:dlxV+[HUHy^yB9d’I*
S\xVw D6Sp>+LY8 I h’I<:H0
Hy9xHyo(u{s2r>*|eD9s2s
B 466Sp>+:eD9*dS\xVw =8Sp>
+:H0Hy9*Hyo(xeD9u{B9d’
I*^ l8w*(;H !264H‘’(HyxeD:
zmS\xVw D8S R 466Sl
9 : bcPXoNBhi
xV
N".()$T"
;%&$#$)01S
H0:C
’$(&")"* +, -+.+/01-&
r>s20
2$3/$,$-(/-" +, #$,,"*"/-"
45 # 6768 6764
o( M‘<
N()"KJ$&"
eD9
<+/$#$(
:*+#%-)$+/146=
r>s20
2$3/$,$-(/-" +, #$,,"*"/-"
6768 6764
466 B7BA @ 67I8 ( ? ! O 675B" P 67I6 #I O 67DDA I4=76= @ 44Q=A ( ?
D8 B7CC @ 67IC (F ?9 ! O 6755" P 6744 #I O 67DD8 4D87A= @ CQ86 (F ?
D6 B7IA @ 67II (F ?9 ! O 675C" P 674C #I O 67DD= 4A475= @ DQB5 F ?9
A8 87=A @ 6754 F- ?9 ! O 67CD" P 674B #I O 67DD= 455766 @ 4IQI6 - 9
=8 87CA @ 6745 - 9 ! O 67C=" P 6744 #I O 67DD= 4CB7DC @ DQCD - 9
I7I J3rs+bbjk? !264?@lm{\9
DE 4cFV:78 Nd 4^YZU*FV\D
_o5Zps78lNz3o5(Z*IA*IB*I8 L
p>+*>H‘D_o5(ud =6SY8*psA
B9dFV+D_o5(*C6*II*I6*C8 Lp>+*
D_o5(uw 86S R B8S*48 Lp>+o5(z
s*~w CS*-17FV:wAD_:o5oVx
o5(u1~8A*IA L‘)Zxv*XFVu@
IA LR+N_}slN7_o5‘
+*>H‘D_7_o5‘
_o5‘
3*o5oVxo5(A*MQd5UBc^Y.p
Z>FVp>+D_o5R:HUyQZ_*ps
|9d’IHy1l5l*(;H !264 H‘D_
o5:zmFVw IA Ll
IcxV:78 Nd I^YZU*xV\D_
o5:78W[*>H‘d 466SS\xVp>+
o5(zA*w =A7CCS*D8Sx D6S:xV+D_
o5(]sB 466Sp>+l17xV:}s*o
5oVN>*o5(^}slA8Sx =8SxV+*
D_o5(?I|gxxV+}sB 4I7BBSx
6B4 & ’ ( ) 58"
d ! FV\ "#$!H‘D_o5:78
%&’(! )*+ +,,+-. /, .+01+23.42+ /5 ’+20&53.&/5
/, 61/2+6 /, 6.23&5 "#$!
789*S\xV :$9‘*~Z 7:;:<9:D_o5l
5l*(;H "#$! H‘D_o5:zES\xV
fd =$9Y8*zmxVw !$$9*<89Y+:xV
!mZD_o5l
9 ! OXV"#$%NRSTmKZ[!
&’() ! &*+ +,,+-. /, .+01+2’.32+ /4 .*+ 1’.*/5+46-6.7 ’5’648. ! ) "#$%&’()**’+ /, 8.2’64 "#$%
FV
)+01+23.42+>?
/bI?( @/22+-. 0/2.3A&.B>9
7 C D C E C 8 C : C < C F C
G)8$ >C
7: !<;
7$ $ D;DD :;:< 7:;:< 8$
!8 $ $ $ $ !;!! 8;8: !$;$$ !7;$:A!! (8E H !7(8
N+A/M(
9 9 PXV"#$%NRSTmKZ[
&’()9 &*+ +,,+-. /, *306:6.7 /4 .*+ 1’.*/5+46-6.7 ’5’648. ! ) "#$%&’()**’+ /, 8.2’64 "#$%
S\xV
PQ>9
/bI?( @/22+-. 0/2.3A&.B>9
7 C D C E C 8 C : C < C F C
G)8$ >C
!$$ !<;
<8 $ :;:< !D;DD D!;!! 8!;!! :<;
>xy%9DE !cFV:78 No E ^YZ
U*H":^Te,D4H‘ 7 ^R*7: ?’Ix
D$ ?’IzqUgI?*|7: ?’II?(%AB
D$ ?’Il17‘<:cy*7$*D8 ?’ISfU
gI?*I?(17‘<:cy^SfwA*! F^
‘7: ?’I:H":^Te,T?I?*! !$ ^
x !!^‘ D$*7$ ?’I)ZT?I?*!8 ?’I
:e,I?z>*!= ^‘pT?I?*/bRI?
(ZO!wlb?*!wFV+‘IB‘ G)8$vr
>u%s2*7: ?‘ G)8$v~w D;D8^*%s2+
d 7 xV\ "#$!H‘D_o5:78
%&’(7 )*+ +,,+-. /, PQ /5 ’+20&53.&/5
/, 61/2+6 /, 6.23&5 "#$!
B9dFV+:‘IB‘lNH":^Te,:z
3I?(x‘IoV G)8$^YZU*(;H "#$!H
‘d 7: ?‘\H":^Te,‘+*z!*XL>
HdlFV+:’(Hyz[*eD9z3*o5o
VsQo5(A$o‘:l
7cxV:78 No 8^YZUq17xV:
}s*hH:H":^Te,I?(^1~}s*‘
IB‘Sf8[lS\xV !$$9:’I‘IoVz
s*F^‘-)ZT?I?*
B9dxV+:‘IB‘*9mwS\xV =89:’
I*F^‘H":^Te,^T?I?*G)8$vw D;=E
^*M9I Dh’I:e,-N_[9’BV-eH
q:zplr>lN‘I‘
!""#:p>+(;H $%"!H‘:‘+*z!l
! \‘’(\s,+WAHH‘HyxeDZi[7
8*b5Umnop(;H $%&!H‘d ’’()X(
g8’(Hyz1*eD9^zA*XLZTJAW0&2**""+cX6$o‘:lbJmnopq
>H‘d *, -+’(Hyz1**. -+eD9z
A*D_o5oVzs*o5(zAl(;H?HD
_:o5xHU:Hy*u%PWA:xV*>H‘
dS\xV /0# 1 !""#:p+Hyz1*d !""#
xV+D_o5oVzs*o5(zAlXxVs
B /"#‘*D_o5oV>*o5(^[[}sl
b5Umn\BX(>H‘Z[[9:D_*x.
fPBHeZo0:B_A
Te,:OFZo0:REY&<**b5UL’
BrZJKWo: 0hFVDVlH":^T$d
1p>X(*dcn9JKxV:‘{%|D_o
55UBxV:cnAV[*Qar^|W[*5l
fgB 0hxV+tlmnopqd *, -‘H":
^Te,I?(<)ZT?I?*OP‘<|W+*
230"vw 4540 6*XL *, -‘>H:o5oVs*o
5(A$So‘:lMxV\‘+*:78LxV
\D_o5:78!qo‘*dWs:xVp>+*
(;H $%&! H‘^ogwWA:‘+**oa3
$^B*+X:1pHy,d+?*LD:+?c9
>?{JK|TPJKB:xV%A*NMZ%B
D_o5Xboa3*bsJ\A3Bu*!//7cO
@*(;H?HD_o5xHUHy*uJ%WA:
S\xVl|Tes,x65w+:S\xV9:
tWn*-cdsB +0#:p>+^
c*cH":^Te,Ood+‘<>M-WAx
V*MD_5~R*oBTes,OX*-^APO
EB:+?x(K*mnHyde*5l*&%<3
w:LV+#:Axp>*YAPRkJKB:+
#Axp>*s%
74
43i*s82?HD_jQEB@D*I]W,
8*@++*ewo)]:Y&,1B*>.vH:
P]s,1)D\Th^T*#;@Tpq"er
(;H:FZ[=Hk*(;HL9d:H7:$
NcP*(;H_KnY&vH{\knn:fP*
dqp>m‘/f(;HHEYHK*^ Ycme
@O:e,
b5U$0:mn*BUBmNH":^TA
‘+0(;H $%"!H‘HyY&Th:X(gx
F*xVp>*wsR:*l4"t0Bo0:g
=*|cJ%\9d785_*%<5U=n"mo
s:DE*YkCNIZ=:fP>H‘l
d e , -
OA~*OE]*>G.*2*!//05 (;H\dRh?^_:V>
‘+*$0 8 BCH7*l*!!A*cq07 9 005
UdA*OE]*OA~*2*!//05 (;H*lM%^_{[5U 8
BCH7*l*!!A7cq!,0 9 !,+5
=)8*;~t*IX6*2**"".5 h?_(;H $:;33<="7H‘2i^T2,:‘+* 8s,)**0!A!cq!"* 9 !"+5
3Bu 8 !//75 s,+I) 8x%qx_(lU4w*4," 9 4,,5
? C 8 *""!5 EO]-X((;H?HD_:DE 8 &’()*4+
A0cq!47 9 !4/5
3Xd*C ‘*O0N*2 8!//+5 cP)H\H":^Te,-g
;,:9sDE 8 &’()DE*!"A!cq/, 9 //5
iao*
=@T 8 !//*5 BCG&s, 8 /WqBC&’U4w*700 9 70+5
W0&*a}k*? )*2**""+5 tZa*1o+WHh?_(
;H:?)*$0&9H*~* 8HI)***+A7cq!00. 9 !0,*5
O /*Izc*?_Hp 8 !///5 cd^T+WAH:4rx‘+
*DE 8 /W&’[))***!A7cq,+ 9 +*5
O0~*iE;*O u*2**""45 H":^T:q+Ws^T:
=_,9sDE 8 &’()*4/A*cq!+! 9 !+45
O:K*uZ^ 8*""45 BCG&R[H7V; 8 /WqBC&’U4
w*4* 9 448
9J@*
)>?@AB: (C D?EEF G H*I@6EJBK: $ G*!///5 LMMCJAF EM NEO AC>PC?:AQ?C ER
S?EOAB P:?:>CAC?F @R ABC CRAE>EP:ABESCR@J MQRSQF !"#$%&’(’)*
$+’,-./’$" I@6EJBK:8 D:R G $@J?ET@EN*70*!"00 9 !",!5
UB:?: VQ>@E* 3EW:>: $:F:AEFB@* %:AE 3:KC?Q**""45 ’:ABESCR@J@AW EM
!"#$%&’(’)* $+’,-./’$" AE ABC JBCFARQA OCCX@N N:?X:C QR6C? N:TE?:AE?W
:R6 M@CN6 JER6@A@ERF8 )PPN LRAE>EN YEEN*4.A7cq7,! 9 7,05
GCRK@RF H L*’?@E? D8 !//75 Z?EOAB :R6 ME?>:A@ER EM A?QC JER@6@: TW
!"#$%&’(’)* 0/$1-1’%’2" @R : F@>PNC N@[Q@6 >C6@Q>8 $WJNES@J:N
%B@>@\Q %QFQ>Q*;:>:]@ $EAEKE**""45 LMMCJA EM 6CRF@AW EM ABC AC?>@AC*
3"#’4)/’#"%*", ,."%$#), ^ENTCA UFEPAC?:q@A@6:Cc* ER ABC
FQFJCPA@T@N@A@CF AE !"#$%&’(’)* $+’,-./’$" 8 )PPN LRAE>EN YEEN*4.A!cq
!*0 9 !4"5
P?ES?:> ME? P?ET@A:R:NWF@F8 IQNN LRAE>EN %EJ )>*A*4cq*"/ 9 *!48
!"#$% ./0,
*,! & ’ ( ) 70"