以探讨马拉巴栗种子脱水耐性与其超氧化物歧化酶（SOD）之间的关联为目的，选用新鲜的成熟马拉巴栗种子，用二乙基二硫代氨基甲酸钠(DDTC，SOD抑制剂）进行12 h预处理后，在25 ℃下进行3～20 h的硅胶快速脱水处理，观察种子的萌发率和生理指标的变化。结果表明：1）20 mmol·L-1能显著抑制种子SOD活性，减弱种子的脱水耐性，表现为种子相对电导率、MDA含量在脱水中后期迅速增加，种子萌发率明显降低，活力指数显著下降；2）种子存在2种MnSOD，4种FeSOD，2种Cu/ZnSOD； 迁移率由大到小依次为Cu/ZnSOD，FeSOD，MnSOD； 谱带由强到弱依次为FeSOD，Cu/ZnSOD，MnSOD；3）经脱水处理，种子中各种SOD同工酶条带的亮度均有不同程度降低，亮度下降最明显的是MnSOD，其次是Cu/ZnSOD，脱水20 h后，除了FeSOD2、Cu/ZnSOD1外，其余条带消失；4） 经20 mmol·L-1DDTC预处理后脱水，种子中所有条带的亮度均明显下降，脱水20 h后，所有条带全部消失。由此推测，马拉巴栗种子脱水耐性与其SOD活性相关。
To investigate the relationship between the tolerance to dehydration and superoxide dismutases(SOD) in Pachira macrocarpa, fresh mature seeds were pretreated with a 12 h DDTC and subjected to 3～20 h desiccation silica gel at 25 °C. The results showed that 20 mmol·L-1 diethyldithiocarbamate(DDTC) treatment inhibited efficiently SOD and SOD activity in seeds and reduced tolerance of seeds to water deficit, which was evidenced with increases in electrical conductivities and malondialchehy of the seeds, and a rapid decrease of the seed vigor during desiccation. There were 2 isoforms of MnSOD, 4 isoforms of FeSOD and 2 isoforms of Cu/ZnSOD in seeds of P. macrocarpa. Their mobility in electrophoresis was Cu/ZnSOD＞FeSOD＞MnSOD, and bands intensity was in the descending order of FeSOD＞Cu/ZnSOD＞MnSOD. During the dehydration, the band intensity of all SOD isoforms was reduced differentially. The most significant reduction was for the band of MnSOD, followed by Cu/ZnSOD. After 20 h, all bands disappeared except for FeSOD2 and Cu/ZnSOD1.When the seeds were pretreated with 20 mmol·L-1 DDTC for 12 h, the band intensity of all SOD isoforms reduced significantly. All bands in the seeds disappeared after 20 h desiccation. It suggested that the tolerance to dehydration would be highly related to SOD activity.
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