采用旷场试验,连续6天观察分析昆明种小白鼠经不同浓度侧柏枝叶挥发气味环境处理后行为指标的变化,研究侧柏枝叶挥发物对小白鼠自发行为的影响,以评价侧柏挥发物的保健功能。结果表明: 1)处理组小白鼠在旷场中的运动总路程大于对照组,且与浓度的大小呈正相关,表明侧柏挥发物有助于提高小白鼠的兴奋性。2)中央格运动总路程、中央格运动时间、站立次数等3项指标呈现阶段性变化,在适应阶段处理组的探究能力大于对照组,熟悉环境阶段则差异不明显,但处理组指标数值的下降幅度远远大于对照组,表明侧柏挥发物在增加小鼠认知能力,提高记忆能力方面起到有利的作用。3)处理组和对照组小白鼠体质量变化相同,但处理组小白鼠的粪便粒数始终比对照组少,说明在侧柏挥发物气体环境中,小白鼠的食欲没有受到影响,精神相对比较轻松。
In order to evaluate the health function of volatile organic compounds (VOCs) from branch and leaf of Platycladus orientalis, effects of the volatile on ICR mice spontaneous behavior were examined for successive 6 days. Using an open-field test the changes of mice behavior indicators were observed under environments with different concentrations of volatile matter. The results were as follows: 1)The total moving distance of treatment group was greater than that of the control group in the open field, and the distance was positively correlated with the concentration of volatile compounds, which demonstrated that P. orientalis volatile stimulated mice. 2) The three indicators, including central grid distance, the central cell exercise time and frequency of standing, presented a stage change. The inquiry ability of treatment group was bigger than the control group at the adaptation stage, however the differences were not significant during the familiar environment stage. Nevertheless, reduction of the indicator values in treatment group was greater than that of the control group, which indicated that P. orientalis volatile played a beneficial role in increasing cognitive and memory abilities of mice. 3)Treatment groups and control group had the same changes in body weight, but the fecal grains of treatment group were always less than the control group, indicating that the P. orientalis volatile odor environment had no influence on mice‘ appetites, and mice were relatively relaxed with the volatile matter.
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