摘 要：利用孝顺竹小穗和种胚为外植体，诱导出胚性愈伤组织并成功实现植株再生。对影响愈伤组织诱导和分化的基本培养基、激素种类及质量浓度等进行筛选，结果表明： 愈伤组织诱导以NB，N6基本培养基附加4 mg·L-12，4-D较好； 外植体在NB＋500 mg·L-1脯氨酸＋500 mg·L-1谷氨酰胺＋300 mg·L-1水解酪蛋白＋30 g·L-1蔗糖＋8 g·L-1 卡拉胶（Carrageenan）＋4 mg·L-1 2，4D的诱导培养基上经20 d培养，小穗愈伤组织诱导率达87.30%，种胚愈伤组织诱导率为76.27%。愈伤组织预分化及分化的基本培养基以MS较适宜，经MS＋30 g·L-1蔗糖＋10 g·L-1卡拉胶＋4 mg·L-1 KT培养基预分化7 d后，转入无激素的MS培养基上分化14 d，小穗愈伤组织仅个别长出绿色芽头； 但种胚愈伤组织芽分化率可达80.0％。分化芽中有8％左右的白化苗，并伴有花叶苗出现。优化后的种胚愈伤组织预分化最佳培养基是MS＋3 mg·L-1 6-BA＋3 mg·L-1 KT。分化芽苗在添加2 mg·L-1 NAA的MS培养基上生根良好，移栽成活率可达70%以上。
Abstract： Embryogenic callus was induced and plant regeneration had been achieved by culturing spikelets and embryo explants of Bambusa multiplex. Basal media，phytohormones and their concentration were screened，and the results showed that NB and N6 basal medium supplemented with 4 mg·L-1 2，4dichlorophenoxyacetic acid (2，4-D) were relatively better for callus induction. A 87.30% spikelets and a 76.27% embryos were induced to generate callus on NB medium supplemented with 500 mg·L-1 proline，500 mg·L-1 glutamine，300 mg·L-1 hydrolyzed casein，30 g·L-1sucrose，8 g·L-1 carrageenan and 4 mg·L-1 2，4-D in 20 days. Murashige and Skoog (MS) basal medium was effective for embryoids‘ predifferentiation and germination. After a 7 d predifferentiation on MS supplemented with 30 g·L-1 sucrose，10 g·L-1 carrageenan and 4 mg·L-1 kinetin (KT) and a 14 d auxinfree germination culture on MS，only a few spikelet calli germinated； whereas embryo calli regenerated by up to 80.0％. Around 8% plantlets were albinal，and mosaic plantlets were occasionally found. Optimal predifferentiation medium for embryo embryoids was MS supplemented with 3 mg·L-1 6benzylaminopurine (6-BA) and 3 mg·L-1 KT. MS supplemented with 2 mg·L-1 naphthaleneacetic acid (NAA) was effective for the plantlet rooting. Rooted plantlets transferred to soil with over 70% success.
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