Guisang 12, a variety of mulberry (Morus alba), was used to study the effects of different levels of water hardening on physiological characteristics of the mulberry seedlings in the hydro-fluctuation belt of the Three Gorges Reservoir Area. The seedlings were subjected to different water regimes for 30 days. The results showed that: the chlorophyll content of mulberry seedlings was increased by 10.4% under the drought hardening (DH) condition than that of CK, while decreased by 3.2% under the waterlogging hardening (WH) condition than that of CK. With the soil moisture content reducing, the stomatal conductance (Gs) and transpiration rate (Tr) of mulberry seedlings both declined remarkably; The photosynthetic rate (Pn) of mulberry seedlings under the drought hardening and waterlogging hardening conditions was all higher than that of CK, and the intercellular CO2 concentration (Ci) under the two conditions was lower than that of CK; The instantaneous water use efficiency of mulberry seedlings under the drought hardening and waterlogging hardening conditions was remarkably increased by 57% and 27% than that of CK, respectively, and the abscisic acid (ABA) content in the two conditions were remarkably increased by 79% and 29% than that of CK, respectively.
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