对米槠和苦槠两林木树种的保存材料进行LN2 保存,采用不同含水量(MC)、冷冻方式和解冻方式处理,并对保存后种子发芽率、电导率、SOD活性和MDA含量进行了跟踪测定。结果表明:LN2 保存的技术关健是在于减少保存材料在脱水过程中造成的脱水伤害,其次是采用适宜的冷冻和解冻方式。米槠种子和离体胚最适含水量均为12%MC ,采用MF-QT方式较为适宜;苦槠种子最适含水量为15%MC ,其离体胚保存时,可适当降低含水量到12% ,MF -QT方式较为适宜。
By using different moisture content (MC), freezing and thawing methods, conservation materials of two tree spices, Castanopsis carlesii and C. kawakamii, were immersed in liquid nitrogen and then their germination, conductivity, SOD activity and MDA content were traced to measured. The results showed that in cryopreservation process, MC control was the main and firstly factor, and secondly were proper freezing methods and thawing methods. The optimal MC of both C. carlesii seeds and excised embryos were 12%MC, and MF-QT methods. Optimal MC of C. kawakamii seeds was15%MC, but of its excised embryos was properly down to 12%MC, MF-QT methods.
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