运用LN2( - 196℃)保存手段,结合添加和不添加防冻剂预处理,通过对锥栗种子LN2 保存前后种子质量特性和胚酶活性的分析,对其长期保存的可行性进行研究,结果表明:含水量是影响锥栗种子和离体胚LN2 保存的重要因素,LN2 保存应进行适度脱水。当无防冻剂预处理时,LN2 保存宜采用15%～20%含水量区间,冷解冻方式采用MF MT。当有防冻剂预处理时,各种酶活性的保存要比无预处理的要好,LN2 保存宜采用20%～25%含水量区间,冷解冻方式采用MF ST。
By means of cryopreservation(-196℃)pretreated with cryoprotectants or not, characteristics of seed quality and enzyme activity of embryos were measured and analyzed both before and after cryopreservation, and long term storage feasibility was studied. The results showed that moisture content (MC) was the main factors deciding the cryopreservation effects of both C. henryi seeds and excised embryos. Moreover, the treatment desiccating down to a medium MC should be taken in cryopreservation process. Under the conditions of no cryoprotectants pretreatment, the optimal group of treatments was 15%～20% MC,mild freezing, and quick thawing. Otherwise, enzyme activity protected by cryoprotectants was higher than that of none, and the optimal group of treatments was 20%～25%MC, mild freezing, and slow thawing.
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