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作 者 ：王学聘 韩一凡 戴莲韵 李玲 田颖川

期 刊 ：林业科学 1997年 33卷 1期 页码：69-74

关键词：欧美杨；苏云金杆菌毒蛋白基因；抗虫转基因植株；

Keywords:P.×euramericana, B.t β-endotoxin gene, Insect-resistang transgenic plants,

摘 要 ：以携带35S-Ω-B.t-Nos嵌合基因的双元载体农杆菌pB48.214和pB482.15(B.t基因片断长度分别为2.1kb和1.8kb),转化欧美杨叶片和茎段外植体,共获得225株转化再生植株。以舞毒蛾(Lymantria dispar Linnaeus)幼虫进行生物测定后,获得杀虫率分别为55—80%的抗虫转基因植株共3株(R-135,R-123和R-140),经组培苗和苗圃移栽植株的PCR分析,苗圃移栽植株PCR产物杂交和Southernblot分析表明,B.t毒蛋白基因已整合到上述抗虫植株的细胞DNA中,并表达出杀虫活性。

Abstract:Leaves and stem segments of P．×euramricana transformaed with A.Tumefaciens hauboring binary vectors pB48.214 and pB48.215 containing chimeric genes of 35S-Bt-Nos with different lengths of B.t gene respectively．Three plants selected by southern blot analysis showed IPC toxicity against larvae of Lymantria dispar．Based on the results of PCR analysis,southern blot of PCR products and genomic DNA as well as insect bioassay,three transformed plants were confirmed to be insect-resistant transgenic plants of P.×euramericana

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