Abstract:Gas chromatography (GC) and High-pressure liquid chromatography (HPLC) were used to detect the phytohormones produced by Boletus sp. and Suillus grevillei. Both fungi could produce ethylene and zeitin but had differences in metabolic activities, and B. sp. could secrete zeitin out of hyphal cell but S. grevillei could not do so.In the bioassay of antibiotic activity using 4 ectomycorrhizal fungi, Boletus griseus and Laccaria amethystea demonstrated their ability to produce intracellular antibiotics against Rhizoctonia solani.