Abstract:A study on the allelic nature of random amplified polymorphic DNA (RAPD) Marker was made with femalegametophytes of half-sib seeds from single tree of Masson‘s Pine. On the basis of screening the primers for amplification effects, the Mendelian 1: 1 segregation was tested for amplified products of 40 seeds from C-16 tree of Zhong Yi population with 17 primers. The results showed that the number of amplified fragments for each primer ranged from 7-22, and the total number of amplified fragments over 17 primers was 201. Among these, there were 129 heterozygous bands which occupied 64 percent of total fragments. Through χ2 test (P<0. 05), 55 fragments (Loci) fit the 1: 1 segregation of Mendelian, which were 27 percent of total amplified fragments, These research results will provide the Mendelian RAPD marker for testing genetic variation analysis of single tree or populations and linekage map construction in Masson‘s Pine.