Abstract:The effective regenetation system for Populus deltoides was developedfirstly in the experiment.Based on the system,Agrobacterium tumefaciens LBA 4404 strains hauboring the Bt.toxin gene expression vectors pB48.214and pB48.215 respectively were uesd for transformation of poplar plants bymeans of the leaf disc method.After transformating,adventitious buds wereformed at the cut of explant on the media with two concentrations of kanamycin 50mg/l and 30mg/l nearly one month later.Variance analysis showedthat the effects of two kanamycin concentrations on the transformation rateand mean adventitious bud numbers were significant at p<0.05.When shootsreached 1cm long,they were transferred to rooting media and supplementedwith 30mg/l and 50mg/l kanamycin and the rooting percentage was 9.62%and 8.02% respectively.PCR analysis showed that Bt.genes were successfully transformed into Populus deltoides chromosomes.