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作 者 ：于在林 王贵成

期 刊 ：林业科学 1987年 23卷 2期 页码：221-226

关键词：核型；

摘 要 ：作者试验将杨尺蠖核型多角体病毒经0.02M Tris-HC1(pH7.6)—0.005MEDTA配制的尿素、SDS和NaCl分别处理后,达到较好的分离效果。采用蔗糖梯度离心和Sepharose 2B柱层析法分离纯化的病毒粒子和核衣壳,经10%聚丙烯酰胺凝胶电泳检测,纯化的病毒粒子和核衣壳不能进入凝胶,胶板无蛋白带着色。理化性质分析表明,多角体中DNA含量约6μg/mg,不含RNA。对完整多角体作16种氨基酸含量分析,总回收率为77.09%。病毒粒子和核衣壳的紫外吸收光谱各具特征。病毒柱子的超离心沉降系数为S₍₂₀₎^w=705,同时还有一个S₍₂₀₎^w=57的组分存在。对所得结果作了讨论。

Abstract:In this paper, we present the purification and analysis of some features of nuclear polyhedrosis virus of poplar looper,Apocheima cinerarius. The polyhedrosis inclusion bodies(PIB)were treated respectively by urea, SDS and NaC1 in 0.02M Tris-HCl (pH7.6)-0.005MEDTA buffer. The Virions, released from PIB treated with 0.05M NaCl+0.05M Na₂CO₃,and the nucleocapsids, released from virions treated with 2% Nonidet p-40 (Np-40), were purified with the techniques of sucrose density gradients centrifugation and Sepharose 2B gel column chromatography. Thevirions and nucleoeapsids are different in ultraviolet absorption spectrums. The purity of virions and nucleocapsids was tested by using 10% polyacrylamide gels electrophorese. The process of PIB solution was observed by scanning and transmission electronic microscopy. The analysed results show that DNA content is about 6μg per mg of dry PIB. The determination of 16 kinds amino acids composition of PIB shows that the recovery rate is about 77.09%. The sedimentation coefficient of virions is about 705(S₍₂₀₎^w), and a 57(S₍₂₀₎^w) component part was observed at the same time. The results are discussed in this paper.

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