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作 者 ：张星耀 赵仕光 朴春根 吕全 贾秀贞

期 刊 ：林业科学 1999年 35卷 3期 页码：34-40

关键词：树木；溃疡病病原真菌；28S rDNA-PCR-RFLP；RAPD；分类学；

Keywords:Tree, Canker pathogenic fungi, 28S rDNA-PCR-RFLP, RAPD, Taxonomy,

摘 要 ：小穴壳菌属(Dothiorela Sac.)(有性阶段是葡萄座腔菌属Botryosphaeria Cas.)、疡壳孢属(Dothichiza Lib.)、壳囊孢属(Cytospora Ehrenb.)、盾壳霉属(Coniothyrium Sacc.)等4属真菌是引起树木溃疡病的主要病原真菌类群,是我国现阶段森林的重大有害生物。本文报道了这4个属的真菌基于28SrDNA-PCR-RFLP和RAPD解析的分类学研究结果,15个供试菌株聚为4类,每一类即一属,在分子水平上证明了其属的传统分类地位的客观性。

Abstract:The fungal group, including the genera of Dothiorella Sacc. (its sexual phase is Botryosphaeria Cas.), Dothichiza Lib., Cytospora Ehrenb. and Coniothyrium Sacc. presently are the most important forest pathogens in China. In this paper, the tested fifteen isolates of the group are divided into four clusters by the methods of 28S rDNA-PCR-RFLP and RAPD analyses. Each cluster just corresponds to one genus. So, the objectivity of the traditional taxonomy of these genera is proved in the molecular level.

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