Abstract:The strong nematicidal activity of aloperine from Sophora alopecuroides L. against pine wood nematode (Bursaphelenchus xylophilus Stener et Buhrer Nickle) is reported for the first time. The nematicidal activity of aloperine against pine wood nematodes was examined by the bioassy in which the medium containing aloperine was used to culture mycelia of Botrytis cinerea Pers.After 5 days LC= 2. 63 × 10-5g/ml. Moreover, the nematicidal activity of aloperine increased with time. When the levels of aloperine in the bioassy medium were at 1 × 10-4g/ml and 2. 63 × 10-5g/ml,the nematicidal effects after 15 days reached 99. 9% and 94. 3%, respectively. The signifcance of alkaloids from S. alopecuroides in studies on the control of pine wilt desease is discussed.