Abstract:Certain dose of aloperine was injected into the basal trunk of 6～7 years old healthy Black pine,Pinus thunbergii in the late June or early July.In the preventive test one week or two weeks after the injection of aloperine,in the therapeutical test one week or two weeks before the injection,an inoculation was received by each test tree with pinewood nematodes,Bursaphelenchus xylophilus (Steiner et Buhrer) Nickle.Results were checked in the late March of the following year.Recovery from the disease was ascertained by resin exudation and growth and survival of the lead shoots.In the preventive test at the doses of 0042,0124g/tree,the survival rates of the test trees were 43%,68% respectively.In the therapeutical test at the doses of 0042,021g/tree, the survival rates were 50%,938% respectively.The survival rates of control trees of the two tests were 30% and 20% respectively.Aloperine is the first natural compound, the effects of which in controlling the pine wilt disease have been confirmed in the field tests.Aloperine dosage used in the future practice of controlling pine wilt disease,its toxicity to human and tree,production cost and perspective in practice were discussed.