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作 者 ：石胜友;梁国鲁;成明昊;郭启高;李晓林;周志钦

期 刊 ：园艺学报 2005年 32卷 5期 页码：802-806

关键词：变叶海棠；AFLP；起源；杂交种；

Keywords:Malus toringoides Hughes, AFLP, Origin, Hybrid,

摘 要 ：利用4对AFLP引物组合( EcoRⅠAAC +M seⅠCAC, EcoRⅠACG +M seⅠCAG, EcoRⅠACC + MseⅠCTA, EcoRⅠACT +MseⅠCAG) 对花叶海棠、陇东海棠和变叶海棠不同的变异类型进行分析, 共得到扩增位点231个, 其中多态性位点211个, 多态性比例为91.34% , 区分率达100%; 利用AFLP标记对变叶海棠、陇东海棠、花叶海棠的亲缘关系进行分析, 结果与形态学、细胞学和同工酶的结果一致, AFLP数据表明变叶海棠是花叶海棠和陇东海棠的杂交种, 从而在分子水平上揭示了变叶海棠的杂种起源。变叶海棠的变异类型是在漫长的进化过程中变叶海棠与花叶海棠和陇东海棠产生渗入杂交的结果。

Abstract:We studied the relationship ofM alus toringoides Hughes and its parents by applying AFLP. 231 polymorphic bandswere obtained with four AFLP primers, and among them 211 (91.34% ) bands were polymorphic with an average of 5718 perAFLP p rimers combination. The relationship s among M. toringoides
Hughes, M. transitoria Schneid and M. kansuensis Rehd were analysed with AFLP. The results are consistent with previous studies based on morphological, cytological and isozyme data. AFLP marker data show that M.toringoides Hughes has been the hybrid of M. transitoria Schneid and M. kansuensis Rehd, and that the variation types of M. toringoides Hughes have been the results of introgressive hybridization between M. toringoides Hughes and M. transitoria Schneid or between M. toringoides Hughes and M. ansuensis Rehd.

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