Abstract:We studied the relationship ofM alus toringoides Hughes and its parents by applying AFLP. 231 polymorphic bandswere obtained with four AFLP primers, and among them 211 (91.34% ) bands were polymorphic with an average of 5718 perAFLP p rimers combination. The relationship s among M. toringoides Hughes, M. transitoria Schneid and M. kansuensis Rehd were analysed with AFLP. The results are consistent with previous studies based on morphological, cytological and isozyme data. AFLP marker data show that M.toringoides Hughes has been the hybrid of M. transitoria Schneid and M. kansuensis Rehd, and that the variation types of M. toringoides Hughes have been the results of introgressive hybridization between M. toringoides Hughes and M. transitoria Schneid or between M. toringoides Hughes and M. ansuensis Rehd.