摘 要 ：选取了11种国内外冬青园艺品种或种, 以其离体叶片为试材, 采用低温循环仪自动降温程序模拟冰冻处理, 用电导法测定相对电导率, 并配以Logistic方程, 计算出各自的半致死温度。试验结果显示11种冬青的抗冻能力由高到低依次为: 龟甲冬青( Ilex crenata var. convexa) 、绿冬青( I. viridis) 、拉索里娜冬青( I. ×altaclerensi‘Lawsoniana’) 、枸骨( I.cornuta) 、冬青( I. chinensis) 、无刺枸骨( I. cornuta var. fortunei) 、金边小叶冬青( I. ×altaclerensis ‘Golden Van Tol’) 、毛冬青( I. pubescens) 、花刺冬青 ( I. aferox ‘Avgentea’) 、小果冬青( I. micrococca) 和金宝石冬青( I. crenata ‘Golden Gem’) 。在此基础上选出9种冬青树种离体叶片为试材, 作多个低温处理, 测定其可溶性糖和游离脯氨酸含量。结果表明, 随处理温度的降低, 可溶性糖和游离脯氨酸的含量逐渐增加, 低温下可溶性糖和游离脯氨酸的含量仅与部分冬青的抗冻性呈正相关。该研究数据对推测引进新品种的抗冻能力, 为它们的合理应用提供科学依据。
Abstract:Electric conductivities in detached-leaf of eleven cultigens of Ilex from domestic and abroad were under simulated frozen treatment. Lethal temperature 50 (LT50 ) was calculated with logistic formula and the results revealed that the frozen resistance from strong to weak were Ilex crenata var. convexa, I. viridis, I. ×altaclerensis ‘Lawsoniana’, I. cornuta, I. chinensis, I. cornuta var. fortunei, I. ×altaclerensis ‘Golden Van Tol’, I. pubescens, I. aferox ‘Avgentea’, I. m icrococca, I. crenata ‘Golden Gem’. Nine cultigenswere selected based on the above results and their leaves were detached and treated at different low temperature to measure the contents of sucrose and p roline. The results indicated that with temperature decreasing, the contents of soluble sucrose and proline increased, and the contents were positively related to the frozen resistance in some cultigens. The research results demonstrated important indexes to predict the frozen resistance of other new introduced plants.