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作 者 ：郭英华;关秋竹;张振贤

期 刊 ：园艺学报 2005年 32卷 5期 页码：905-907

关键词：姜；悬浮系；体细胞胚；植株再生；

Keywords:Ginger, Suspension culture, Somatic embryogenesis, Plant regeneration,

摘 要 ：选用四川省地方品种‘竹根姜’的茎尖组织建立了体细胞胚性细胞悬浮系, 并通过悬浮培养获得了姜再生植株。结果表明悬浮细胞干质量受pH影响。接种量和AgNO₃ 对悬浮细胞的生长都有影响:最佳接种量为1.0% , 最适AgNO₃浓度为6.0 mg·L ^{- 1}。胚性细胞悬浮系增殖后, 接种到前期筛选的成熟分化培养基上获得了再生植株。

Abstract:Somatic embryogenic suspension cultures of‘Zhugenjiang’ginger cultivars of Sichuan province were established. From which, regenerated plantlets were obtained. The relationship between the dry mass of suspension cultures and pH changes in medium was also discussed. The effects of inoculation and
AgNO₃ on the growth of embryogenic suspensions were also discussed. The optimum concentrations of inoculation and AgNO₃ were 1.0% and 6.0 mg·L^-1 , respectively.

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