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作 者 ：肖　艳;周厚高;黄子锋;王凤兰

期 刊 ：园艺学报 2005年 32卷 3期 页码：524-526

关键词：新铁炮百合；RAPD；遗传分化；遗传多样性；

Keywords:Lilium formolongi, RAPD, Genetic differentiation, Genetic diversity,

摘 要 ：采用RAPD技术研究了新铁炮百合(Lilium formolongi) 自交初代的遗传多样性变化, 建立了
自交系纯合分子判断标准。研究结果表明, F₂、F₃、F₄ 世代株系多态性百分比分别为59.1%、56.6%、48.0% , 遗传多样性指数分别为1.749、1.530、1.292, 平均遗传距离分别为0.121、0.207、0.238。在总变异中, 世代内的分布Hpop /Hsp和世代间分布(H_sp-H_pop) /Hsp遗传多样性所占的比率分别为76.2%和23.8%。

Abstract:In Order to monitor the homozygous progress of inbred breeding in molecular level and to establish homozygousmolecular criteron of inbred lines of Lilium formolongi, the genetic diversity in self-inbred early generations of Lilium form olongiwas studied by RAPD. The results indicated that the percentage of polymorphic bands of F₂ , F₃ and F₄ generations were 59.1%, 56.6%, 48.0% respectively, the Shannon genetic diversity indeces 1.749, 1.530, 1.292 respectively, the mean genetic distances 0.121, 0.207, 0.238 respectively.
Among total variation, intrapopulation genetic diversity accounted for 76.2%, interpopulation for 23.8%.

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