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The Effect of NH4+-N on Anatomical Structure of Alfalfa Conducting Tissue

NH4+-N对紫花苜蓿输导组织解剖结构的影响



全 文 :!23" !5#
 Vol.23  No.5
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ACTA AGRESTIA SINICA
   2015$  9%
  Sep.  2015
犱狅犻:10.11733/j.issn.10070435.2015.05.009
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d–ÇܨBñ 

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+

©3e‰ŠBIàHËñ*Ìïw,•NH+4NܨG210mg·L-1“,
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678

-o;NH+4N;êNàH;¿§l%;IO
9:;<=
:S541    >?@AB:A     >CD=:10070435(2015)05096305
犜犺犲犈犳犳犲犮狋狅犳犖犎+4犖狅狀犃狀犪狋狅犿犻犮犪犾犛狋狉狌犮狋狌狉犲狅犳犃犾犳犪犾犳犪犆狅狀犱狌犮狋犻狀犵犜犻狊狊狌犲
HAOFeng,LIUXiaojing,ZHANGXiaoling
(ColegeofGrasslandScience,GansuAgriculturalUniversity,KeyLaboratoryofGrasslandEcosystemofMinistryofEducation,
SinoU.S.CentersforGrazingLandEcosystemSustainability,Lanzhou,GansuProvince730070,China)
犃犫狊狋狉犪犮狋:GannongNO.3wasusedasexperimentalmaterialswithfivedifferentNH+4Nconcentrations
(0,105,210,315,420mg·L-1).Anatomicalstructurecharacteristicsofthreetypesoforgans(roots,
stemsandleaflets)wereobservedbyusingparaffinsectiontechniquesandphotomicrography.Theresults
showedthatvasculartissuethroughouttheroots,stemsandleafletswasincreasedatthebegining,and
thenreducedwiththefurtherincreaseofnitrogentreatment.WhenNH+4Ntreatmentwas210mg·L-1,
thefunctionofconductingtissueachievedthebestperformancewithmorevessels,moremultivascular
bundles,largerandbetterdevelopedxylemandphloemareaaswelasmorexylemconduits.Changesin
othertissuesofvegetativeorganswerealsoobserved,therootscortexwasthickedandparenchymacel
volumedecreasesd.Theintramedularycavityhadflakytissueandtheareawasincreased.Leavesmidrib
becamemoreprominentandepidermisconsistedofalayerofoblong,morecompactlyarrangedepidermal
celswithincreasedNH+4Nconcentrationwithinarangeof0to210mg·L-1.Theoptimalconcentration
wasat210mg·L-1.FurtherincreaseoftheconcentrationofNH+4Nconcentrationreducedtheimprove
menteffectsinstead.
犓犲狔狑狅狉犱狊:Alfalfa;NH+4N;Conductingtissue;Anatomicalstructure;Palisadetissue
EFGH
:20150430;IJGH:20150831
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(1985),Ë,X0nFG»,ƽ˜™:,˜™NLG,Náâ,Email:haofeng1026@163.com;ÇvÅÆ Authorfor
correspondence,Email:liuxj@gsau.edu.cn
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•:u&#!¥ NH+4N|Îì-
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Ü9ùŸ=ÇŸ
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™Ç‡UkWXܨNH+4Nš-oêNàHl%RSB˜™

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u×3cm쓓{,”Éû§30áPi{。¼
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UVæ5eܨKL(0,105,210,315,420
mg·L-1),ƒ5e^X,CM15ü。-o×3®M©“,”ÉAYZ{âBmL。60dÌ,zƘx1^X8¼、+、©‘,
¼¤Gá$ÝBƒX¤Y

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Ç^X8CK(0
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¤/ÂBñ*/¨=IOƒŠX

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Do–Ä—rKL
(犘<0.05)(P1)。¿¼BML
¿§l%…~

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@:HƒžBn<

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•
210mg·L-1^X,IO@Tï,/Â*‰ÕÄE
G

hù¤

C@¤@Ä

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®hBDàH

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ëÄŊõ

šCK=420mg·L-1^XzÆñ*
182.38%=100.69%,1^X’“BDo–Ä—r
469
!5# À !!:NH+4NK-oKL
(犘<0.05)(P2)。hù¤Ýܪ.,hù¨ì

Ò/šCK=420mg·L-1^XzÆñ*
321.96%=276.61%。C@¤@Ä,Ò/ÂBñ*
/¨tW†hù¤

wÒn<ª«5ŠXB

P2)。
¿+BML¿§l%…~

• NH+4NܨG210
mg·L-1“,êNàHl%Ävzh。
\1 犖犎+4犖%hâ3=;<=>@lÐ0 ¡
Table1 EffectofNH+4NonrootsstructureonGannongNo.3
^X
Treatments/mg·L-1
IO/Â
Vascularbundlearea/μm2
hù¤/Â
Xylemarea/μm2
C@¤/Â
Phloemarea/μm2
@æH¨
Cortex/μm
Np³
Vesselnumber
0 185501±656.58e 17643±50.72d 167857±707.31e 106±0.98e 46±1.20e
105 696028±366.96b 52168±79.14b 643861±373.22b 187±0.54c 107±1.20b
210 369614±516.60c 35281±51.49c 334332±565.89c 247±0.87b 82±1.20c
315 991558±389.03a 75575±61.01a 915984±449.35a 290±0.78a 122±1.45a
420 263759±760.83d 16867±64.96e 246892±809.42d 138±0.63d 51±1.20d
  ò:P+XÄWXwéBCPóDo—r(犘<0.05)。šX
Note:Differentlowercaselettersinthesamerowmeansignificantdifferenceatthe0.05level.Thesameasbelow
:1 ‡ˆ犖犎+4犖jk¾hâ3=@fg­qŠ
Fig.1 AnatomicalchangesofrootsfromGannongNo.3exposuretodifferentNH+4Ntreatments
ò

×1×200;¬¸=50μm;A:CK0mg·L-1Ç^X;B:105mg·L-1Ç^X;
C:210mg·L-1Ç^X;D:315mg·L-1Ç^X;E:420mg·L-1Ç^X。šX
Note:Fig.1×200;Scalebars=50μm;A:CK0mg·L-1nitrogentreatment;B:105mg·L-1nitrogentreatment;
C:210mg·L-1nitrogentreatment;D:315mg·L-1nitrogentreatment;E:420mg·L-1nitrogentreatment.Thesameasbelow
\2 犖犎+4犖%hâ3=;<=>GlÐ0 ¡
Table2 EffectofNH+4NonstemsstructureonGannongNo.3
^X
Treatments/mg·L-1
IO/Â
Vascularbundlearea/μm2
hù¤/Â
Xylemarea/μm2
C@¤/Â
Phloemarea/μm2
IOep
Vascularbundlenumber
Np³
Vesselnumber
0 7561±52.48e 1835±70.93e 5725±95.76d 8.67±0.33a 63±2.65e
105 15557±60.21c 7109±45.24b 8448±101.62c 11.00±0.58a 100±1.20c
210 17475±74.99b 4748±51.43c 12727±43.64b 14.00±1.15b 119±1.53b
315 21351±77.13a 7743±63.22a 13608±16.59a  14.33±1.20b 132±2.08a
420 10639±45.92d 2056±71.10d 8583±25.31c 10.00±0.58a 68±1.45d
:2 ‡ˆ犖犎+4犖jk¾hâ3=Gfg­qŠ
Fig.2 AnatomicalchangesofstemsfromGannongNo.3exposuretodifferentNH+4Ntreatments
2.3 犖犎+4犖¹$%ÝlÐ0 ¡
Ç^X8CKƒÈ,¾EXê/¨š*,P@ë
u,€ÕğGEGBœæüýàá

IO/š
*

hù¤ªØš*üýL HBhùN

×3)。
WXܨ NH+4N^X,K©®¾EIOB
l%

RÆ5hù¤

C@¤B67š*

•210
mg·L-1^Xš,IO/Âv*,hù¤Ýܪ
.

Np³v‹

¾E"êJ—

×3)。IO/
šCK=420mg·L-1^XzÆñ*185.05%=
81.51%,hù¤zÆñ*186.81%=50.55%,C
@¤/ÂzÆñ*184.64%=76.24%,1^X’
“BDo–Ä—rKL
(犘<0.05)(P3)。¿©B
569
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•NH+4NܨG210mg·L-1“, êNàHl%Ävzh。
\3 犖犎+4犖%hâ3=;<=>ÝlÐ0 ¡
Table3 EffectofNH+4NonleafletsstructureonGannongNo.3
^X
Treatments/mg·L-1
IO/Â
Vascularbundlearea/μm2
hù¤/Â
Xylemarea/μm2
C@¤/Â
Phloemarea/μm2
¾E"ê¨
Midribprocess
Np³
Vesselnumber
0 5145±38.86e 1107±39.08d 4037±60.47d 1.75±0.018d 11.0±0.58d
105 11733±43.79b 1781±60.16b 9951±103.65b 2.33±0.018b 18.3±0.88b
210 8484±21.40c 1871±74.23b 6613±95.00c 2.34±0.009b 15.7±0.88c
315 14666±36.68a 3175±54.23a 11491±90.74a 2.48±0.012a 24.7±0.88a
420 8080±41.09fd 1570±74.60c 6520±109.04c 1.99±0.012c 13.3±0.67c
:3 ‡ˆ犖犎+4犖jk¾hâ3=Ýfg­qŠ
Fig.3 AnatomicalchangesofleafletsfromGannongNo.3exposuretodifferentNH+4Ntreatments
3 ~Yl
¼A5\Ë¢ìËÌâzÌën
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¼¤KŸ7¾êBâç

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uȨ!
[19]
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PJ¼BIÈÊ*s89êNàH@Ä

¾ê
âç›

TUVÕ•NH+4NÄÅ210mg·L-1
“

-o
EJ-ozBÏÐ*¥i5qvì

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H*‘

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WXNH+4Nܨš-oBížgn<;iH˜™

};ŠŒIJ-oBâz¾ê*¥›X

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+LJKDàHBl%¿n…ížFŽBn<

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210mg·L-1“DNf®hBDàH,+“V“a,
/Âv*

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âŸïB*¥¼ÏÐBâz=©ú:B›9ù

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©589;i?яâB¾D‰Š

Öç 
!
[21]
vGFŽn%@:ƒžn<

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[22]
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©®àHl%8:Žn<=89BåýâçPA
GöBƒ@g

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NH+4NK¾©EB67šUC。89Ç8ÆK<
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üý+

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‘*¦:u“Í*¥Ð÷
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[23]。
T˜™Õ-oܨG210mg·L-1“^¦vãh,|Îìܨ
imˆÊÚ

¿¿§l%ã¨EJHs¤€B
n<8NH+4NܨBƒJ@A,ÃKT[’à0
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GKs
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u>?
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