免费文献传递   相关文献

Cloning and Transformation of MsMYB2 Gene from Medicago sativa L.

紫花苜蓿MsMYB2基因的克隆及转化



全 文 :!23" !3#
 Vol.23  No.3
! " # $
ACTA AGRESTIA SINICA
   2015$  5%
  May.  2015
犱狅犻:10.11733/j.issn.10070435.2015.03.021
öö÷ø 犕狊犕犢犅2K‡oª«1­)
»9¼
1,
» .1,½¾[2,8 ¿1,À*Á1
(1.8¸®÷à.]4ÌÍn,„À100193;2.„ÀW÷à.W./,„À100083)
67

_ôK犕狊犕犢犅22ü;"+}I}2üvTä(犃狉犪犫犻犱狅狆狊犻狊狋犺犪犾犻犪狀犪)ž6,£¤PCR„…fXL\]
(犕犲犱犻犮犪犵狅狊犪狋犻狏犪)8ëìq犕犢犅22üPíé_ 犕狊犕犢犅2,犕狊犕犢犅22üä@÷834bp,ä1N·e_
278Aü2½IøÚJ。:;DNA~«„…W|¶pBI121öO,nó›Õ‰ž&+}”2pBIMsMYB2。:;
Lu¾ð³ôKðâWmk¥^ñI}2üvTäž6

P:;PCRjRTPCR<¥I2ü5Wö,€a{›
¥I2üéïØvTä2ü«8PçvP+}

nóôK‰犕狊犕犢犅22ü+}IvTä}2üž6。
89:

XL\]
;犕狊犕犢犅22ü;vTä;};<,-=
:Q943.2    >?@AB:A     >CD=:10070435(2015)03058006
犆犾狅狀犻狀犵犪狀犱犜狉犪狀狊犳狅狉犿犪狋犻狅狀狅犳犕狊犕犢犅2犌犲狀犲犳狉狅犿犕犲犱犻犮犪犵狅狊犪狋犻狏犪犔.
FENGZirong1,SUNYan1,CHAOYuehui2,YUANZhu1,LUJixiao1
(1.GrasslandResearchInstituteofChinaAgriculturalUniversity,Beijing100093,China;
2.TheColegeofForestry,BeijingForestryUniversity,Beijing100083,China)
犃犫狊狋狉犪犮狋:Agenenamed犕狊犕犢犅2wasclonedfromalfalfabyPCRtechniquewith834bpcodingdomainse
quenceencoding278aminoacids.The犕狊犕犢犅2genewasinsertedintoplasmidpBI121byDNArecombi
nationtechnology;andaplantexpressingvectorpBIMsMYB2wasconstructed.Theplantsoftransgenic
犃狉犪犫犻犱狅狆狊犻狊狋犺犪犾犻犪狀犪withkanamycinresistancewerescreenedthroughfloraldipmethodandanalyzedby
PCRandRTPCR.TheresultsshowedthatthetargetgenewasinsertedintothegenomesofArabidopsis
andexpressed.Therefore,thetransgenicArabidopsisthalianaplantsexpressing犕狊犕犢犅2geneweresuc
cessfulyobtainedinthisstudy.
犓犲狔狑狅狉犱狊:犕犲犱犻犮犪犵狅狊犪狋犻狏犪L.;犃狉犪犫犻犱狅狆狊犻狊狋犺犪犾犻犪狀犪;犕狊犕犢犅2gene;Transformation
EFGH
:20140521;IJGH:20140716
KLMN
:¸
¹*]Í÷„…2ù
(CARS35)ß¿;¸ ¹“´ Ãê”-„º»¼^r½¾“;-QŸ?-*]Â`œG„…ÌͶÅL”ß
¿
(2011BAD17B0102)ÀÁ
OPQR

—r~
(1990),á,Èq§,úËÌÍñ,ÏÐ*]G;j`rñÍÌÍ,Email:pipishan@yeah.net;:6x× Authorfor
correspondence,Email:ctsoffice@163.com
  MYB}ür˜ž&}ür¹^8Izà
¹^g¥

xyJ¶ž&’;jځI)A֎

m
VJ¶ž&ñ·

D¥6UäŸj®‰Ú¸ø
֎ID]
[13]。
ž&8I MYBa}ürˆH
|n¯IMYB€›‘G",vJ_Ç¥MYB€›
‘ëÒ
(singleMYB)、2Aå˜ MYB€›‘ëÒ
(R2R3MYB)j 3 A å ˜ MYB € › ‘ ë Ò
(R1R2R3MYB)3A&a。MYB€›‘˜Ñeé
ŠI€›‘

‘A€›‘8â50~53Aü2½Ê
2

MnÎ}$ÎI›b。MYB@‘ëÒf
ž&Mò–—ID,8⥑5(IHI
[4]。
ž&
8I!1A MYB}ür˜Ï[h(犣犲犪犿犪狔狊
L.)8’,I,vPD]J¶L&¥ñ&n€›
2üI+}

Pçä‰Uâ犮犿狔犫û€2üéŠ
DNA€‘IëÒ,iýØ ABAID][56]。g
¢
,犕犢犅I¬·2ü‚fàE(犎狅狉犱犲狌犿狏狌犾犵犪狉犲
L.)[7]、ʁ]Ð(犃狀狋犻狉狉犺犻狀狌犿犿犪犼狌狊L.)ž&[8]、
Z‚JLÐ
(犘犲狋狌狀犻犪犺狔犫狉犻犱犪Vilm)ž&[9]、vTä
(犃狉犪犫犻犱狅狆狊犻狊狋犺犪犾犻犪狀犪)[1011]j41ƒ_Ð(犘犺狔
狊犮狅犿犻狋狉犲犾犾犪狆犪狋犲狀狊)ž&[12]8’,。
ž& MYB2}ür˜Š¹^8I¥A4&
^

‹Œž&8 犕犢犅2Ióô‹Œ,J¶­ŒD¥
 D

~ñځD,

(4#

]OJ\;S

f
}Éu†D]#ž&‹ŒÉùIñ·’;

MYB2}ürÐÞ R2R3MYB a}ür。
MYB2}ürfvTä8Ÿà"ÌÍ。1998$,
!3# —r~:XL\]犕狊犕犢犅22üIëìQ}HoerenFU[13]IÌÍ+›,犃狋犕犢犅2:;€f
¥‘HˆIuæJ¶¡p.­ID]

‹Ÿ<‘¡
p¬·2üI+}

è$:¬·ÌÍ+›
[1418],
MYB2}ürf¹4#、Õ4#PQ ABA6
U}Ÿ;S8ù’”#~Vx¤
。2013$,Baek

[19]
:;2üvP:;D! miR399fI}D,vTä<
Þń…IÖ­

˜ž&2å()Åu)IÅ6U
:µI¥UJ

PçöO#Åu)j NaCl,ABA
6’;D]<‘™ñ&4#

f|ýñ&8
,MYB2}ür#ø~ŸÌÍ。
2008$,Yu[20]’,犕犢犅22üfòo÷O!"J
\n{†I;S8’”~Vx¤

˜Y{NëÒj|
ýå?2üâ>I.µD!ür
。2011$,3¸´

[21]
:;<Æû
(犛犪犮犮犺犪狉狌犿狊犻狀犲狀狊犻狊Roxb)MYB2}
ürIÌÍ+›

Æû犕犢犅22ü÷_991bp,
|8Y¯570bpÛӇˆ>Pä189Aü2½,Y
¯ MYBIóô‘。:;Œ?ñJK’,ÆûI
犕犢犅22ü¶|ýž&ß[hfuæ«n、Ñy€
›j!ñSÝ֎ðâvÑI¬Ùñ

犕犢犅22üf¸øž&8ŸºàÌÍ,-|fX
L\]8Ióôϋç‰

?íîPXL\]_þ
â

ëìq 犕狊犕犢犅22ü,öOØ+}”2pBI121
†

:;®c}\IÖ³Ÿ¬vTä8+}

Ïæ
ôK犕狊犕犢犅22ü;"+}I}2üvTäž6。
1 STUVW
1.1 ST
íîn¤XL\]8\¥U
(犕犲犱犻犮犪犵狅犛犪狋犻狏犪
‘ZhongmuNo.1’)` r_8¸®÷-./„À)*
+,ÌÍn]÷ÌÍÞéî

²RNA¦“í)®、À
ÁŽÂDNAªÓí)®、Àq4¦í)®Š_„
Àʋfñ&-„â@›r
;PCRMixQ“Žˆ"ð
âUltraSYBRMixtureŠ_Ì_ØÙñ&-„â@
›r
;DNA Marker、犜犪狇 DNA l£、ëì”2
pEASYT1Š_„ÀõÊñ&-„â@›r;
DNaseA、.}í)®、T4DNAöO£、)`@O
ñåK£Š_TaKaRa›r;àŒcƒýòDH5α,
Š_„ÀȈñ&-„â@›r

+}”2pBI121
_8¸®÷-./„À)*+,ÌÍn]÷ÌÍÞ
éî

|ýí)v°¤5µJ©6¸ÍJK²

1.2 Ö×VW
1.2.1 ÂrµF ˆH˸¸1ñ&„…6789
(NationalCenterofBiotechnologyInformation,NCBI)
†¦RI}M\]犕犢犅22ü(GenBankρU:XM
_003591311)uæ,‹¼¥MYB2r¤Þ2üëìQ+}”2›Õ;ˆHž&+
}”2pBI121Iuæ,‹¼¥<:¤d&,pBIfj
pBIr,ð2uæß+1nÅ。|8^-¶I_@Oñ
åK£犡犫犪Ij犛犿犪II£KSÝ。
q1 ùº¨©
Table1 Thesequencesofprimers
é9
Name

Sequence
MYB2f5’CCCTCTAGAATGGATACTAATTACAAAACC3’
MYB2r5’GGGCCCGGGTAAATCATCAGCCAATTGTTGC3’
1.2.2 ÃÄÅÆ 犕犢犅2ºÇ@ÈÉ £¤²
RNA¦“í)®¦“XL\]² RNA,:;
RNaseAìü2ü«DNAEð¢,.}_!1
JcDNA。P!1JcDNA _´x,MYB2fj
MYB2r_d&,5W PCR œù。œùSu_:
95℃a—ñ10min;95℃—ñ30s,55℃[i30s,
75℃?ß1min,30A1;75℃?ß5min;4℃ƒ
yéî
。PCRÍ&í0.8%€üÀÁŽÂö,
£¤ÀÁŽÂªÓí)®²\¢

öOØëì”2
pEASYT1,}\àŒcƒýò+ÍDH5α,Ž
f¯â50mg·L-1WÕk¥ILB"«2†,37℃
"«;Ï

<“Çëìcš

5Wc2PCRö,S
u_
:95℃a—ñ3min;95℃—ñ30s,55℃[i
30s,72℃?ß90s,30A1;72℃?ß5min;4℃
ƒyéî

WŠñëìØ„ÀÈ¥>µñ&„…
â@›ru

1.2.3 krPÊÓz{ £¤DNA ManÉO<
u€a5WJK

Pa|ä@Sò

£¤
DNA Man j MEGA 5.1 É O < ë ì q I
犕狊犕犢犅22ü5WJK+›|Œ?ñ,P›Õ‰5
\。
f ExPaSyPE (http://web.expasy.org/
compute_pi/)aŽÝjJr"。:;SignalP
4.1Server(http://www.cbs.dtu.dk/services/
SignalP/)a|6UÚFG。£¤PlantPLocR
ð
(http://www.csbio.sjtu.edu.cn/bioinf/plant/)
a|&+͈SFG

1.2.4 Jrs†¸$@Ë+ p“¯âl‡
犕狊犕犢犅2uæIàŒcc6,¦“Àq。P¯â
¥ I 2 ü I pEASYMYB2 j ž & + } ” 2
pBI121_<Ä,¤ 犡犫犪Ij犛犿犪I5Wó£K,
37℃.­2h。£KÍ&í;ÀÁŽÂö¢,:
185
! " # $ !23"
;ÀÁŽÂªÓí)®²\¥I.ù

¤T4DNA
öO£16℃öO6h,P}\ØàŒcƒýò
+Í8

PŽf¯â50mg·L-1WÕk¥ILB
"«2†


Yc

PpBIfjpBIr
_d&

5Wc2PCRö。p“PCRöŠñ
c6

¦“Àq

:;犛犿犪Ij犡犫犪I@OñåK£
5W£K@ˆ

1.2.5 ÌÍÎ{,FÏA £¤Àq4¦í)®
¦“pBIMsMYB2Àq,¤CaCl2Zê³}\Ö³
Ÿ¬®c GV31018。<}\I®c5W
PCRöJK,£¤LuH\³,<ËÞL#I
BñbvTä5WË5

ð2zÊ_

¤éOÁ2I
¯âpBIMYB2ž&+}”2ÀqI®ccn
<éí·q‘ñLuIvTäž65W¾ð

ÉH
\2min,’†îâ:vé)3e, =Ë51d¢
~®ÓòاRxÿR8"«

Óô}2üvTä`r

í75%I~”Ï¡10
min¢,vyÝfC¯â50mg·L-1Wmk¥1/2
MS"«2†,Ô`r’‚,p“ôÛlšñˆIv
Tä}%CYB8‰°"«

p“ðâWmk¥^
ñvTä2.

¦“2ü« DNA,P2ü« DNA
_´x

£¤ MYB2fj MYB2r_d&,5W
PCRö;p“ŠñvTä}2üž&,¦“²
RNA,.}_cDNA ¢,PcDNA _´x,£¤
MYB2fj MYB2r_d&,5WPCRö。PCR
Í&í1%€üÀÁŽÂJK。
2 mnU,c
2.1 öö÷ø犕犢犅2K‡oª«
PX L \ ] cDNA _ ´ x,P MYB2f,
MYB2r_d&,5WPCRœù,PCRÍ&í€
üÀÁŽÂœÅnóôK‰1NÉ_800bpà4
IDNA NU(‰1)。WŠ.ùöOØëì”2
pEASYT1P}\àŒcƒýò+Í,íc2
PCRö,p“¯â¥INUIÇëìc2ñ&
›ru

u€aœÅ

nóôK‰¯â”ï
犕狊犕犢犅22üä@,ÿD2ߗ,/â’ñ%ä
ߗ

Wul‡IÀqíé_pEASYMsMYB2。
2.2 Ѻ}~,,c
ëìKØI2ü÷_834bp,|¯â1A”
ïIä@

ä1N·e_278Aü2½IøÚ
J

<ôKI MsMYB2ü2½uæ5Wñ&67
<1 öö÷ø犕狊犕犢犅2K‡犘犆犚æ‰
Fig.1 PCRamplicationof犕狊犕犢犅2genefrom
犕犲犱犻犮犪犵狅狊犪狋犻狏犪
•
(Note):M:Trans2KTMPlusⅡDNA{Á;M:Trans2KTMPlusⅡ
DNAMarker;1:犕狊犕犢犅22ü;1:犕狊犕犢犅2gene
.JK+›

ÏXL\]8J‚qI MsMYB2¶
Ï|ý&`8J‚qI MYBëÒ,ðâSÑIŒ
?ñj¬ÙIéŠ@

‰2、‰3)。fExPaSyPE
(http://web.expasy.org/compute_pi/)aŽ
ÝjJr"

J*_
:5.90/31.88KDa。íSignalP
4.1Server(http://www.cbs.dtu.dk/services/
SignalP/)a,‹îf6UÚ(‰4)。&+͈S
aœÅ
,MsMYB2ˆSÞ+Íë8。
2.3 ˆºqÑL/à‡
¦“ÀqpEASYMsMYB2Àq,¤犡犫犪Ij
犛犿犪I5Wó£K,ªÓ.ù¢öOCpBI121”2
†

}\àŒcDH5αƒýò,<“Çcšc2
PCR¢,íÀÁŽÂöKÀ:¯ â¥I~«rI
c2vôœùq¶¥I2üà4¥ŠIDNA NU

‰5)。¦“PCRöŠñcnÀq,¤犡犫犪Ij
犛犿犪I5Wó£K,£KÍ&í1%€üÀÁŽÂ
JKKÀ

f800bpËîf¥NDNANU,¶_¬
2üà4¥Š

‰6),+›ž&+}”2›Õnó,
PJ*íé_pBIMsMYB2。
2.4 #Œú­)1»m
P2 ü « DNA _ ´ x,£ ¤ MYB2f j
MYB2r_d&,5WPCRö,PCRÍ&í€
üÀÁŽÂJKKÀ

npI136ðâWm^ñI
vTäž6

|812Aôۜùq¥I2üà4
DNANU,æd}2üj‰ñ<àv‹ôôKŠ
DNANU(‰7);PcDNA _´x,£¤ MYB2f
j MYB2r_d&,5WPCRö,PCRÍ&í
€üÀÁŽÂJKKÀ

npI126PCRöŠ
ñ}2üvTäž6

|88Aôۜùq¥I2
üà4 DNA NU,æ|846‹ôœùq¥I
DNANU(‰8),P†€a+›nóôK‰}
犕狊犕犢犅22üvTä}2üž6,æç 犕狊犕犢犅2
2üôÛf}2üvTä8nó+}

285
!3# —r~:XL\]犕狊犕犢犅22üIëìQ}<2 犕狊犕犢犅2ÐÄÂUf‘ˆº#ÐÄo¨©“Á
Fig.2 ComparisonofaminoacididentitiesoftheMsMYB2proteinfromalfalfaandotherdifferentplants
<3 犕狊犕犢犅21#ÐÄÆ)‘,c
Fig.3 PhylogeneticanalysesofMsMYB2andorthologs
<4 犕狊犕犢犅2ÐÄ}=ûüm
Fig.4 SignalpeptidepredictionofMsMYB2protein
<5 狆犅犐犕狊犕犢犅2Iýþc犘犆犚,c
Fig.5 PCRanalysisofEschherichiacoliharboringpBIMsMYB2
•
:M:Trans2KTMPlusⅡDNAMarker;1~9:pBIMsMYB2c2PCR
Note:M:Trans2KTMPlusⅡDNAMarker;
1~9:PCRamplicationofpBIMsMYB2
<6 狆犅犐犕狊犕犢犅2Àÿ!®
Fig.6 EnzymeanalysisofpBIMsMYB2
•
:M:Trans2KTMPlusⅡDNAMarker;
1:pBIMsMYB2ó£K
Note:M:Trans2KTMPlusⅡDNAMarker;
1:pBIMsMYB2digestedwith犡犫犪Iand犛犿犪I
3 œ
fž&I}D,8
,犕犢犅¹^_LH2üóô
IãR:ˆ¨JrýO¦R‰¥Av2I´b

õö
f×&jkc8óôS4

-fÛLž&8犕犢犅2ü
I¹^´J—àæçøF[1,2223]。
ž& MYB2}
ür˜Š¹^8I¥A4&^

‹Œž&8犕犢犅2I
óô‹Œ

-ùf}Éu†D]#ž&‹ŒÉùI
ñ·’;
。MYB2}ürfvTä85W‰à"Ì
385
! " # $ !23"
Í

Pç’,Š2üi¶^añ¬·

!›犕犢犅22ü fù/ž&^añ֎âºòIÌÍá…。
<7 ­K‡#Œú犘犆犚»m
Fig.7 PCRanalysisoftransgenicArabidopsisplants
•
:113:}2üvTä;CK:‰ñ<à;M:Trans2KTMPlusIIDNAMarker
Note:113:TransgenicArabidopsis;CK:negativecontrol;M:Trans2KTMPlusIIDNAMarker
<8 ­K‡#Œú犚犜犘犆犚»m
Fig.8 RTPCRanalysesoftransgenicArabidopsisplants
•
:112:}2üvTä;M:Trans2KTMPlusIIDNAMarker
Note:112:TransgenicArabidopsis;
M:Trans2KTMPlusIIDNAMarker
  ?íînóÏXL\]8ëì‰犕狊犕犢犅22
ü

P›Õ‰”2

:;ñ&67.JK’,

犕狊犕犢犅22üäëÒ¶|ýž&ëÒðâSÑ
IŒ?ñ

íSignalP4.1Server(http://www.
cbs.dtu.dk/services/SignalP/)a,‹îf6U
Ú

<¶3¸´
[21]
<ÞÆûIÌÍPQ~“¶

[24]
<Þ&SV
(犃狊犻犪狋犻犮犮狅狋狋狅狀)IÌ̀a¬ã。
&+͈SaœÅ
,MsMYB2ˆSÞ+Íë8,
<¶~ÿ
[16]
<ÞvTäIÌ̀a¬ã
。pBI
MsMYB2ˆ+}”2I›Õ;S8,:;PCRœ
ù

vPf¥I2üÛӇˆ>âøJ*d¬犡犫犪
Ⅰj犛犿犪Ⅰ£KSÝ,‡éöO;S8_¬ÖƒI
l‡ñ

vTä˜2üóô@ˆI´µž&

:;
}2ü„…W2ü}¬vTä85W¬·ö

#
˜@ˆdÀóô2üI¥`š¤Ö³

?íî:;
®cpŸÖ³}\vTä

:;PCRjRTPCR
Ö³<}2üvTäž65Wö

€aœÅnp
136!ñž6â86ôœùq¥INU,æd}2
üj‰ñ<àv‹ôôKŠDNANU,+›nó
ô K ‰ } 犕狊犕犢犅2 2 ü v T ä ž 6,æ ç
犕狊犕犢犅22üôÛf}2üvTä8nó+}。
‡ˆž68˜,nó}¬¥I2üUV:;2`Ö
³¬€:Œö

í;Wmk¥ºp¢ôlš
ñˆIž68

ö¢7â‡UJdnó}¬¥I


vô˜ÝÞºpI"«28Wmk¥J‹
v6מ6ñˆ;S8Íñ‰¥ˆIWm^ñnd
ïI

vní:;©eùàWmk¥‡e:ݽ

4 m
PXL\]_þâ

ëìq 犕狊犕犢犅22ü,ö
OØ+}”2pBI121†,:;®c}\IÖ³Ÿ
¬vTä8+}

nóôK‰犕狊犕犢犅22ü+}I
vTä}2üž6

žŸ>?
[1] RabinowiczPD,BraunEL,WolfeAD,犲狋犪犾.MaizeR2R3
Mybgenes:Sequenceanalysisrevealsamplificationinthe
higherplants[J].Genetics,1999,153(1):427444
[2] Frohman,MichaelA,MichaelK,犲狋犪犾.Rapidproductionof
fullengthcDNAsfromraretranscripts:amplificationusinga
singlegenespecificoligonucleotideprimer[J].Proceedingsof
theNationalAcademyofSciences,1988,85(23):89989002
[3] ArabidopsisGenomeInitiative.Analysisofthegenomese
quenceofthefloweringplantArabidopsisthaliana[J].Na
ture,2000,408(6814):796815
[4] Doebley,John,LewisLukens.Transcriptionalregulatorsand
theevolutionofplantform[J].ThePlantCelOnline,1998,
10(7):10751082
[5] PazAresJ,GhosalD,WienandU,犲狋犪犾.Theregulatoryc1
locusof犣犲犪犿犪狔狊encodesaproteinwithhomologytomyb
protooncogeneproductsand withstructuralsimilaritiesto
transcriptionalactivators[J].TheEMBOJournal,1987,6
(12):35533558
[6] HattoriT,VasilV,RosenkransL,犲狋犪犾.TheViviparous1
geneandabscisicacidactivatetheC1regulatorygeneforan
thocyaninbiosynthesisduringseedmaturationinmaize[J].
Genes&Development,1992,6(4):609618
[7] MaroccoA,WissenbachM,BeckerD,犲狋犪犾.Multiplegenes
aretranscribedinHordeumvulgareandZeamaysthatcarry
485
!3# —r~:XL\]犕狊犕犢犅22üIëìQ}theDNAbindingdomainofthemyboncoproteins[J].Molec
ularandGeneralGenetics,1989,216(23):183187
[8] JacksonD,CulianezMaciaF,PrescottAG,犲狋犪犾.Expression
patternsofmybgenesfrom Antirrhinumflowers[J].The
PlantCel,1991,3(2):115125
[9] AvilaJ,NietoC,CaasL,犲狋犪犾.Petuniahybridagenesrelat
edtothemaizeregulatoryC1geneandtoanimalmybproto
oncogenes[J].ThePlantJournal,1993,3(4):553562
[10]OppenheimerDG,HermanPL,SivakumaranS,犲狋犪犾.A
mybgenerequiredforleaftrichomedifferentiationinArabi
dopsisisexpressedinstipules[J].Cel,1991,67(3):483493
[11]ShinozakiK,YamaguchiShinozakiK,UraoT,犲狋犪犾.Nucleo
tidesequenceofagenefromArabidopsisthalianaencodinga
mybhomologue[J].PlantMolecularBiology,1992,19(3):
493499
[12]LeechMJ,KammererW,CoveDJ,犲狋犪犾.Expressionof
mybrelatedgenesinthemoss,Physcomitrelapatens[J].
ThePlantJournal,1993,3(1):5161
[13]HoerenFU,DolferusR,WuY,犲狋犪犾.Evidenceforarolefor
AtMYB2intheinductionofthe犃狉犪犫犻犱狅狆狊犻狊犪犾犮狅犺狅犾犱犲犺狔犱狉狅
犵犲狀犪狊犲gene(ADH1)bylowoxygen[J].Genetics,1998,149
(2):479490
[14]ZhengZL,NafisiM,TamA,犲狋犪犾.Plasmamembraneasso
ciatedROP10smalGTPaseisaspecificnegativeregulatorof
abscisicacidresponsesin犃狉犪犫犻犱狅狆狊犻狊[J].ThePlantCelOn
line,2002,14(11):27872797
[15]AbeH,UraoT,ItoT,犲狋犪犾.ArabidopsisAtMYC2(bHLH)
andAtMYB2(MYB)functionastranscriptionalactivatorsin
abscisicacidsignaling[J].ThePlantCel,2003,15(1):6378
[16]~ÿ,¹Š8,–Ó“.vTä ATMYB2}ürIcDNA
uæJKQ|+͈S
[J].ÆÇ®÷à..¸,2009,44(5):
9699
[17]AlexandreC,MlerSteinbachY,SchnrockN,犲狋犪犾.Arabi
dopsisMSI1isrequiredfornegativeregulationoftheresponse
todroughtstress[J].MolecularPlant,2009,2(4):675687
[18]GuoY,GanS.AtMYB2regulateswholeplantsenescenceby
inhibitingcytokininmediatedbranchingatlatestagesofdevel
opmentinArabidopsis[J].PlantPhysiology,2011,156(3):
16121619
[19]BaekD,ParkHC,KimMC,犲狋犪犾.TheroleofArabidopsis
MYB2in miR399fmediated phosphatestarvationresponse
[J].PlantSignaling&Behavior,2013,8(3):36273
[20]HuangYC,SuLH,LeeGA,犲狋犪犾.Regulationofcystwal
proteinpromotersbyMyb2inGiardialamblia[J].Journalof
BiologicalChemistry,2008,283(45):3102131029
[21]3¸´,”bÏ,¸ º),.Æû MYB2}ürIŽrë
ìjñ&67.JK
[J].ñ&67.,2011,9(1):2427
[22]MartinC,PazAresJ.MYBtranscriptionfactorsinplants
[J].TrendsinGenetics,1997,13(2):6773
[23]RiechmannJL,HeardJ,MartinG,犲狋犪犾.Arabidopsistran
scriptionfactors:Genomewidecomparativeanalysisamong
eukaryotes[J].Science,2000,290(5499):21052110
[24]~“¶,~xÁ,ì,.¥A&SV MYB¹^®2üIë
ìQHIJK
[J].8¸®÷-.,2013,46(1):195204

%&( , -)
585