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Physiological Characteristics of Apocynum venetum under the Stress of Drought and Heat

干热胁迫对罗布麻幼苗生理特性的影响



全 文 :!23" !5#
 Vol.23  No.5
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ACTA AGRESTIA SINICA
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  Sep.  2015
犱狅犻:10.11733/j.issn.10070435.2015.05.034
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-0.3,-0.6,-0.9,-1.2,-1.5MPa)BPEGknK=Î1i{;ié:Ü9`aUV,jkÒ:X:<«¬
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犘犺狔狊犻狅犾狅犵犻犮犪犾犆犺犪狉犪犮狋犲狉犻狊狋犻犮狊狅犳犃狆狅犮狔狀狌犿狏犲狀犲狋狌犿狌狀犱犲狉狋犺犲
犛狋狉犲狊狊狅犳犇狉狅狌犵犺狋犪狀犱犎犲犪狋
ZHANGYanyan,ZHUYuhao,MUZhen,WANGZhongmei,OUChengming,RONGYuping
(ColegeofAnimalScienceandTechnology,ChinaAgriculturalUniversity,Beijng100193,China)
犃犫狊狋狉犪犮狋:Toestimatethedroughtandheattoleranceof犃狆狅犮狔狀狌犿狏犲狀犲狋狌犿,seedlingswereirrigatedby
PEGindifferentwaterpotentialsof0,-0.3,-0.6,-0.9,-1.2,-1.5MPa,underthedifferencetem
peraturesof25,30,35℃,respectively.Differentphysiologicalindicesincludingtheelectronicconductivity
(RPP),superoxidedismutase(SOD)activity,solublesugar(SS)andmalondialdehyde(MDA)contentsof
leafweremeasured.TheresultsshowedthatRPPincreasedwiththeincreaseofdroughtandheatstress.
ThecontentsofSSincreasedfirstlyandthendecreasedwiththedroughtandheatstressincreased,andthe
contentsofMDAhadanoppositetrendtothecontentsofSS.FortheactivityofSOD,itincreasedwith
thedecreasingofwaterpotential,anddeclinedwiththeincreasingoftemperature.Alofthesevariation
suggestedthattheseedlingof犃狆狅犮狔狀狌犿狏犲狀犲狋狌犿hadthehighestresistanceunderthestressof-0.9~
-1.2MPain30℃.
犓犲狔狑狅狉犱狊:犃狆狅犮狔狀狌犿狏犲狀犲狋狌犿;Droughtandheatstress;Physiologicalcharacteristics
EFGH
:20140717;IJGH:20150416
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(1989),Ë,&4W»,¼½˜™:,¾D¿ÀNñ:˜™,Email:Zhang_YanYanM@163.com;ÇvÅÆ Author
forcorrespondence,Email:rongyuping@cau.edu.cn
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(solublecarbohydrate,SS)Ìë。
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Ó
[6]
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(malondialdehyde,MDA)Ìë。
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0.01),35℃šDo—r(犘<0.05)。
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Table1 Theeffectsofdroughtandheatstressonrelativeconductivity
^X
Treatments
Kä Waterpotential/MPa
0 -0.3 -0.6 -0.9 -1.2 -1.5 犉
25 0.023cB 0.018bB 0.017bB 0.025cB 0.025bB 0.039bA 9.172
_¨ 30 0.083aD 0.051aCD 0.085aCD 0.102aC 0.143aB 0.192aA 19.153
Temperature/℃ 35 0.060bB 0.059aB 0.083aB 0.067bB 0.138aAB 0.191aA 4.097
犉 75.621 21.983 29.192 80.326 11.669 6.504
  ò:WXwéBCGXŠÄõ“Dog;WX*éBCGXŠiõ“Dog;Pó•0.05KL—r,Pó•0.01KL—r。šX
Note:Valueswithincolumnfolowedbythedifferentlowercasesaresignificantdifference(犘<0.05);Valueswithinlinefolowedbythe
differentcapitallettersaresignificantdifference(犘<0.05);indicatessignificanceatthe0.05level;indicatessignificanceatthe0.01lev
el.Thesameasbelow
  25℃,30℃š,XK}ƒÈ,dKz`aBñ ,
©®RPP˚Ê̂e,0~-1.2MPaš RPP
Do×r
(犘<0.01),-1.5MPašDo—r(犘
<0.05)。©®ƒK³N5¦30℃,-1.5MPaB
Ü9`ašÄÅv*õ

G0.192。
2.2 H¢©ª¾*+Ѓ1û3¦(SS)ø°0qŠ
XŠ_¨š

XK}ƒÈ

|kgxÌëdKä
BÊÚ`\f‚egä

P2)。-0.3 ~-0.6
MPa}†-1.2MPaK䧨šDo×r(犘<
0.01)。XŠKäš,30℃^Xš89\‚|kgx
Ìë`\‚—r
(犘<0.05)ì¦K},ˆ35℃Bì
_`asÑ|kgxÌë×r
(犘<0.01)Ú¦K
}

|kgxÌë¦30℃,-1.2MPaB`ašÄ
Åv*

v*õG21.04mg·g-1。
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Z3qŠ
0~-0.9MPaš,30℃,35℃^Xš89\‚
SODPg–×r(犘<0.01)Ú¦K} (P3),X
Š_¨š

1K䧨^XšB89\‚SODPg
–ì¦K}

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r
(犘<0.01)。SODPg¦25℃,-0.6MPašÄ
Åv*õ507.001U·g-1FW。
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Table2 Theeffectsofdroughtandheatstressonsolublesugarcontent mg·g-1
^X
Treatments
Kä Waterpotential/MPa
0 -0.3 -0.6 -0.9 -1.2 -1.5 犉
25 8.937aA 13.504aA 13.649aA 12.549abA 11.617bA 13.712aA 1.092
_¨ 30 7.949aB 15.871aA 15.711aA 16.217aA 21.037aA 14.547aAB 3.679
Temperature/℃ 35 3.429bC 4.398bC 3.702bC 6.166bB 8.591bA 8.172aA 34.019
犉 81.371 30.453 17.897 4.488 11.526 3.767
\3 H¢©ª%£™¤犛犗犇Z30 ¡
Table3 TheeffectsofdroughtandheatstressonSODactivity U·g-1FW
^X
Treatments
Kä Waterpotential/MPa
0 -0.3 -0.6 -0.9 -1.2 -1.5 犉
25 335.024aC 365.547aC 507.001aA 482.383aA 424.590aB 364.619bC 32.966
_¨ 30 196.366bB 201.086bB 233.682bB 247.485bB 441.081aA 425.750abA 50.605
Temperature/℃ 35 149.621cE 180.084bDE 225.264bD 288.533bC 368.780aB 436.209aA 34.774
犉 109.526 26.667 514.336 83.706 3.316 4.081
2.4 H¢©ª¾*+ЃÛÂÜ(MDA)ø°0qŠ
XŠKäš
,30℃“ MDA Ìë×r(犘<
0.01)Ú¦K}(P4),•0~-0.6MPa,-1.5
MPaKäFŽš,35℃ì_`aÑÕ MDAÌëÃ
—r
(犘<0.01)ì¦K}。XŠ_¨,WXKäš
MDAÌënMDABÌë–ì¦K},‰-0.3MPa,-0.9~
-1.5MPa^XšDo×r(犘<0.01),-0.6
MPašDo—r(犘<0.05)。35℃š,-0.9MPa,
-1.2MPaKä`aÑÕ MDAÌë×rì¦K
}
(犘<0.01)。30℃,-0.9MPa`aš MDAÌ
ëÄÅvÚ

vwõG0.007μmol·g
-1。
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Table4 TheeffectsofdroughtandheatstressonMDAcontent μmol·g
-1
^X
Treatments
Kä Waterpotential/MPa
0 -0.3 -0.6 -0.9 -1.2 -1.5 犉
25 0.015bA 0.013bA 0.015abA 0.017aA 0.017aA 0.017bA 1.106
_¨ 30 0.013bA 0.012bB 0.012cB 0.007bD 0.009bC 0.009cC 15.737
Temperature/℃ 35 0.019aAB 0.017aBC 0.017aBC 0.013aD 0.016aC 0.022aA 10.403
犉 14.319 20.997 5.338 12.270 14.781 31.199
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_
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(犘<0.05),K=Î1i{ MDAÌë、SOD
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(犘<0.01),_¨=KzB
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Table5 Effectsoftemperatureandwaterinteractiononthephysiologicalandbiochemicalindicesof犃狆狅犮狔狀狌犿狏犲狀犲狋狌犿
në=…†
Variableandsource
RPP SS MDA SOD
犉 犘value 犉 犘value 犉 犘value 犉 犘value
_¨Temperature 46.47 0.01 52.371 0.01 68.538 0.01 1366.688 0.01
Kz Water 14.407 0.01 6.029 0.01 5.79 0.01 71.845 0.01
_¨×Kz
Temperature×Water
2.583 0.05 1.847 >0.05 4.919 0.01 23.53 0.01
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[J].4N¸I,2008(7):8790
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+¬£)°+0
,2009:1415,3747
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mutase(SODs)incontrolingoxidativestressinplants[J].Ex
perimentalBotany,2002,53(372):13311341
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¡¢
,2000:167,192,258,260261
[8] H‚©,ÖÆ,±].PEGÜý`aK*oâ5Ni{:XR
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[9] ;¬<,Ö¬¨,}GØ.Üý`aK«Y=Î1!NRà9ù
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[J].ÜýÝ´†8FŽ,2012(12):
177181
[10]kgH,YRÐ,;Ÿ,!.ÿ`aK=Î1Y7²@†i{:
uB67
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