全 文 :!23" !5#
Vol.23 No.5
! " # $
ACTA AGRESTIA SINICA
2015$ 9%
Sep. 2015
犱狅犻:10.11733/j.issn.10070435.2015.05.034
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-0.3,-0.6,-0.9,-1.2,-1.5MPa)BPEGknK=Î1i{;ié:Ü9`aUV,jkÒ:X:<«¬
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犛狋狉犲狊狊狅犳犇狉狅狌犵犺狋犪狀犱犎犲犪狋
ZHANGYanyan,ZHUYuhao,MUZhen,WANGZhongmei,OUChengming,RONGYuping
(ColegeofAnimalScienceandTechnology,ChinaAgriculturalUniversity,Beijng100193,China)
犃犫狊狋狉犪犮狋:Toestimatethedroughtandheattoleranceof犃狆狅犮狔狀狌犿狏犲狀犲狋狌犿,seedlingswereirrigatedby
PEGindifferentwaterpotentialsof0,-0.3,-0.6,-0.9,-1.2,-1.5MPa,underthedifferencetem
peraturesof25,30,35℃,respectively.Differentphysiologicalindicesincludingtheelectronicconductivity
(RPP),superoxidedismutase(SOD)activity,solublesugar(SS)andmalondialdehyde(MDA)contentsof
leafweremeasured.TheresultsshowedthatRPPincreasedwiththeincreaseofdroughtandheatstress.
ThecontentsofSSincreasedfirstlyandthendecreasedwiththedroughtandheatstressincreased,andthe
contentsofMDAhadanoppositetrendtothecontentsofSS.FortheactivityofSOD,itincreasedwith
thedecreasingofwaterpotential,anddeclinedwiththeincreasingoftemperature.Alofthesevariation
suggestedthattheseedlingof犃狆狅犮狔狀狌犿狏犲狀犲狋狌犿hadthehighestresistanceunderthestressof-0.9~
-1.2MPain30℃.
犓犲狔狑狅狉犱狊:犃狆狅犮狔狀狌犿狏犲狀犲狋狌犿;Droughtandheatstress;Physiologicalcharacteristics
EFGH
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Hüü
(1989),Ë,&4W»,¼½:,¾D¿ÀNñ:,Email:Zhang_YanYanM@163.com;ÇvÅÆ Author
forcorrespondence,Email:rongyuping@cau.edu.cn
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[4]。
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5û:9\EPg#ÚÎB)Ët
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5pù#<Å¥Bú9
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áân
。
K{1e%Ì,3Ì<+×PEG6000
G-0.3,-0.6,-0.9,-1.2,-1.5MPaBáâ
n;i^X
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}W PEG6000BáânÅGK}
(CK),e^X3eCM,ò_¨æZG25,30,
35℃B_±}{â»,c8h±},16hX[。
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}ì
[5]
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1.3 4@¥fgUV
B¥³Nð[6]jk©®üýpNg(relative
plasmalemmapermeability,RPP);B¥¦§ÈÞ
Ó
[6]
jk|kgx
(solublecarbohydrate,SS)Ìë。
B¥NBT±øÞÓ[7]jkÐ#<9)
Ó
[6]
jk)Ûy
(malondialdehyde,MDA)Ìë。
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B¥EXCEL2003;ip½(Xô×,¥
SPSS13.0;iôE7NDz{(twowayANO
VA),¥vwrDoÓLSDÈWX^XD
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2 lmY;n
2.1 H¢©ª¾Ýµ|%¥d(RPP)0q
ëP1|ì,XKä,30,35℃^XB©
®RPPì¦K},30℃DoÃr(犘<
0.01),35℃Dor(犘<0.05)。
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Table1 Theeffectsofdroughtandheatstressonrelativeconductivity
^X
Treatments
Kä Waterpotential/MPa
0 -0.3 -0.6 -0.9 -1.2 -1.5 犉
25 0.023cB 0.018bB 0.017bB 0.025cB 0.025bB 0.039bA 9.172
_¨ 30 0.083aD 0.051aCD 0.085aCD 0.102aC 0.143aB 0.192aA 19.153
Temperature/℃ 35 0.060bB 0.059aB 0.083aB 0.067bB 0.138aAB 0.191aA 4.097
犉 75.621 21.983 29.192 80.326 11.669 6.504
ò:WXwéBCGXÄõDog;WX*éBCGXiõDog;Pó0.05KLr,Pó0.01KLr。X
Note:Valueswithincolumnfolowedbythedifferentlowercasesaresignificantdifference(犘<0.05);Valueswithinlinefolowedbythe
differentcapitallettersaresignificantdifference(犘<0.05);indicatessignificanceatthe0.05level;indicatessignificanceatthe0.01lev
el.Thesameasbelow
25℃,30℃,XK}È,dKz`aBñ ,
©®RPPËÊÌe,0~-1.2MPa RPP
DoÃr
(犘<0.01),-1.5MPaDor(犘
<0.05)。©®K³N5¦30℃,-1.5MPaB
Ü9`aÄÅv*õ
,
G0.192。
2.2 H¢©ª¾*+Ð1û3¦(SS)ø°0q
X_¨
,
XK}È
,
|kgxÌëdKä
BÊÚ`\fegä
(
P2)。-0.3 ~-0.6
MPa}-1.2MPaK䧨DoÃr(犘<
0.01)。XKä,30℃^X89\|kgx
Ìë`\r
(犘<0.05)ì¦K},35℃Bì
_`asÑ|kgxÌëÃr
(犘<0.01)Ú¦K
}
。
|kgxÌë¦30℃,-1.2MPaB`aÄ
Åv*
,
v*õG21.04mg·g-1。
2.3 H¢©ª¾*+ÐÞÆ+ßÝ(SOD)
Z3q
0~-0.9MPa,30℃,35℃^X89\
SODPgÃr(犘<0.01)Ú¦K} (P3),X
_¨
,
1K䧨^XB89\SODPg
ì¦K}
,
-0.3~-0.9MPaDoÃ
r
(犘<0.01)。SODPg¦25℃,-0.6MPaÄ
Åv*õ507.001U·g-1FW。
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Table2 Theeffectsofdroughtandheatstressonsolublesugarcontent mg·g-1
^X
Treatments
Kä Waterpotential/MPa
0 -0.3 -0.6 -0.9 -1.2 -1.5 犉
25 8.937aA 13.504aA 13.649aA 12.549abA 11.617bA 13.712aA 1.092
_¨ 30 7.949aB 15.871aA 15.711aA 16.217aA 21.037aA 14.547aAB 3.679
Temperature/℃ 35 3.429bC 4.398bC 3.702bC 6.166bB 8.591bA 8.172aA 34.019
犉 81.371 30.453 17.897 4.488 11.526 3.767
\3 H¢©ª%£¤犛犗犇Z30 ¡
Table3 TheeffectsofdroughtandheatstressonSODactivity U·g-1FW
^X
Treatments
Kä Waterpotential/MPa
0 -0.3 -0.6 -0.9 -1.2 -1.5 犉
25 335.024aC 365.547aC 507.001aA 482.383aA 424.590aB 364.619bC 32.966
_¨ 30 196.366bB 201.086bB 233.682bB 247.485bB 441.081aA 425.750abA 50.605
Temperature/℃ 35 149.621cE 180.084bDE 225.264bD 288.533bC 368.780aB 436.209aA 34.774
犉 109.526 26.667 514.336 83.706 3.316 4.081
2.4 H¢©ª¾*+ÐÛÂÜ(MDA)ø°0q
XKä
,30℃ MDA ÌëÃr(犘<
0.01)Ú¦K}(P4),0~-0.6MPa,-1.5
MPaKäF,35℃ì_`aÑÕ MDAÌëÃ
r
(犘<0.01)ì¦K}。X_¨,WXKä
MDAÌën
-1.5MPa^XDoÃr(犘<0.01),-0.6
MPaDor(犘<0.05)。35℃,-0.9MPa,
-1.2MPaKä`aÑÕ MDAÌëÃrì¦K
}
(犘<0.01)。30℃,-0.9MPa`a MDAÌ
ëÄÅvÚ
,
vwõG0.007μmol·g
-1。
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Table4 TheeffectsofdroughtandheatstressonMDAcontent μmol·g
-1
^X
Treatments
Kä Waterpotential/MPa
0 -0.3 -0.6 -0.9 -1.2 -1.5 犉
25 0.015bA 0.013bA 0.015abA 0.017aA 0.017aA 0.017bA 1.106
_¨ 30 0.013bA 0.012bB 0.012cB 0.007bD 0.009bC 0.009cC 15.737
Temperature/℃ 35 0.019aAB 0.017aBC 0.017aBC 0.013aD 0.016aC 0.022aA 10.403
犉 14.319 20.997 5.338 12.270 14.781 31.199
2.5 i$aó;O%£¤bkb4@0 ¡
P5ó_¨=KzJÅK=Î1:X:<«
¬B67
,
ë£|}~
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zJÅK=Î1i{:X:<\áB67WX
。
_
¨=KzK=Î1i{B©®K³N5rB
öJi
(犘<0.05),K=Î1i{ MDAÌë、SOD
PgÃrBöJi
(犘<0.01),_¨=KzB
öJÅ¥K=Î1i{|kgxÌë67Wr
。
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Table5 Effectsoftemperatureandwaterinteractiononthephysiologicalandbiochemicalindicesof犃狆狅犮狔狀狌犿狏犲狀犲狋狌犿
në=
Variableandsource
RPP SS MDA SOD
犉 犘value 犉 犘value 犉 犘value 犉 犘value
_¨Temperature 46.47 0.01 52.371 0.01 68.538 0.01 1366.688 0.01
Kz Water 14.407 0.01 6.029 0.01 5.79 0.01 71.845 0.01
_¨×Kz
Temperature×Water
2.583 0.05 1.847 >0.05 4.919 0.01 23.53 0.01
3 ~Yl
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K³N5¦35℃zÊ,ÐEJ=Î1
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dÜ9`a¨ñ
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ËÊÌe
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Ð8kgH!
[10]
Kÿ`a=Î
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aâç
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XK}È
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-1.2MPaBÜ9`a=Î1ågÄÅv,
ÌBC¨`a
(35℃,-1.5MPa)i{\â
"EkB9Î
。
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u>?
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perimentalBotany,2002,53(372):13311341
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¡¢
,2000:167,192,258,260261
[8] H©,ÖÆ,±].PEGÜý`aK*oâ5Ni{:XR
gB67
[J].N)°+,2014,31(03):446450
[9] ;¬<,Ö¬¨,}GØ.Üý`aK«Y=Î1!NRà9ù
Â=ûtPgB67
[J].ÜýÝ´8F,2012(12):
177181
[10]kgH,YRÐ,;,!.ÿ`aK=Î1Y7²@i{:
uB67
[J].4N¸I,2012(09):4346
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