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Response of Soil Enzyme Activity and Microbial Biomass Carbon and Nitrogen to the Nutrient Addition of Stipa Baicalensis Steppe in Inner Mongolia

贝加尔针茅草原土壤酶活性及微生物量碳氮对养分添加的响应



全 文 :!24" !1#
 Vol.24  No.1
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ACTA AGRESTIA SINICA
   2016$  1%
  Jan.  2016
犱狅犻:10.11733/j.issn.10070435.2016.01.007
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Authorforcorrespondence,Email:yangdianlin@caas.cn
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:S812    ABCDE:A     AFG@:10070435(2016)01004707
犚犲狊狆狅狀狊犲狅犳犛狅犻犾犈狀狕狔犿犲犃犮狋犻狏犻狋狔犪狀犱犕犻犮狉狅犫犻犪犾犅犻狅犿犪狊狊犆犪狉犫狅狀犪狀犱犖犻狋狉狅犵犲狀
狋狅狋犺犲犖狌狋狉犻犲狀狋犃犱犱犻狋犻狅狀狅犳犛狋犻狆犪犅犪犻犮犪犾犲狀狊犻狊犛狋犲狆狆犲犻狀犐狀狀犲狉犕狅狀犵狅犾犻犪
ZHAOXiaochen1,2,3,HUANGFUChaohe2,3,LIUHongmei2,3,
XIU Weiming2,3,ZHAOJianning2,3,YANGDianlin1,2,3
(1.ColegeofHorticulture,ShenyangAgriculturalUniversity,Shenyang,LiaoningProvince110866,China;
2.EnvironmentalProtectionandMonitoringInstitute,MinistryofAgriculture,Tianjin300191,China;
3.KeyLaboratoryofOriginalAgroenvironmentQualityofMinistryofAgriculture,Tianjin300191,China)
犃犫狊狋狉犪犮狋:Effectsofdifferentnutrientadditionmethodsonthecarbonandnitrogenofsoilmicrobialbio
mass,andenzymeactivityin犛狋犻狆犪犫犪犻犮犪犾犲狀狊犻狊steppewerestudiedinthefieldexperiment.Nutrientaddi
tionmethodsincludedseventypesofN,P,Kfertilizertreatments(CK,K,P,N,PK,NK,NP,NPK)
atthesite.TheresultsshowedthattheinvertaseactivitiesofNP,NPK,P,PKtreatmentsweresignifi
cantlyhigherthanthatofCK,ureaseactivitiesinbothnitrogenandphosphorusadditiontreatments(NP,
NPK)aswelasthealkalinephosphataseactivitiesofNP,NPKandNKtreatmentsimprovedsignificant
ly,thecatalaseactivitiesofaltreatmentshadnosignificantdifference.Themicrobialbiomasscarboncon
tentsofNP,NPK,N,PKtreatmentsweresignificantlyhigherthanothersandthemicrobialbiomassni
trogencontentsofNPtreatmentsweresignificantlyhigherthanthatofCK.Soilavailablephosphoruswas
positivelycorrelatedwithcatalaseandinvertaseactivity,themicrobialbiomasscarbonwaspositivelycor
relatedwithureaseactivityandalkalinephosphataseactivity,andextremelysignificantcorrelationwithin
vertaseactivity.Themicrobialbiomassnitrogenwaspositivelycorrelatedwithsoilavailablephosphorus,
catalase,invertaseandureaseactivity.Theseresultsimpliedthatnitrogenandphosphorusadditiontogeth
ercouldbeusefultoincreasethesoilenzymeactivity,microbialbiomasscarbonandnitrogencontentsof
犛狋犻狆犪犅犪犻犮犪犾犲狀狊犻狊Steppe,andtoimprovethesoilfertilityof犛狋犻狆犪犅犪犻犮犪犾犲狀狊犻狊Steppe.
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`1 c~L>MFNOPš›oQÏÌ¡Q
Table1 Soilphysicalandchemicalcharacteristicsunderdifferenttreatments
Óù
Treatment
pH
×\i
NH+4N/mg·kg-1
Ö\i
NO-3N
/mg·kg-1
Ø:j
AvailableP
/mg·kg-1
àj
TotalP/g·kg-1
JÁF
OM/g·kg-1
CK 6.93±0.03ab 6.83±0.12b 4.43±0.25b 3.94±0.26d 0.34±0.007d 35.92±0.59bc
P 6.69±0.01bc 6.68±0.74b 1.94±0.03e 42.59±2.33ab 0.54±0.023a 45.13±0.59a
N 6.62±0.05c 11.61±0.59a 4.09±0.30bc 4.13±0.29d 0.33±0.020d 36.76±0.44b
K 6.67±0.05bc 3.95±0.79de 3.47±0.34cd 3.62±0.18d 0.32±0.005d 34.58±0.69cde
NP 7.04±0.13a 4.83±0.20cd 3.80±0.24bcd 48.77±5.22a 0.42±0.016b 32.97±0.47e
PK 6.55±0.05c 2.75±0.07e 2.10±0.23e 24.84±2.65c 0.40±0.014bc 35.66±0.47bc
NK 6.74±0.13bc 7.77±1.14b 5.99±0.50a 5.31±0.27d 0.32±0.009d 35.36±0.71bcd
NPK 7.13±0.12a 6.17±0.32bc 3.04±0.21d 34.82±4.98b 0.38±0.011c 33.89±0.60de
  ü:@j6@uU;Çg˜Øº…†(犘<0.05)
Note:Differentlettersinthesamelineindicatesignificantdifferenceatthe0.05level
=1 c~L>MFNOPš›HIÏ
Fig.1 Soilenzymeactivitiesofdifferenttreatments
ü

6@;Çghغ…†
(犘<0.05),œ@
Note:Differentlettersindicatesignificantdifferenceatthe0.05level.Thesameasbelow
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`g»`Ýs
(NP:705.9mg·kg-1,NPK:652.3
mg·kg-1),=¾ÚCKT1.73,1.60±。NPÓù
…†sé N,PK,CK,K,P,NKÓù(犘<0.05),
NPKÓù…†séCK,K,P,NKÓù(犘<0.05),
NP,NPKã%غ6…†。N,PKÓùD?9K
`g»`AJ…†T›s

=¾Ú CK T1.42,
1.40±,ò…†séCK,K,P,NKÓù(犘<0.05),
Iñ™ã%غ6…†
。CK,K,P,NKD=¼î
Óù»`݀

òغ6…†

=2 c~L>MFX9Pš›J,1êK
Fig.2 Soilmicrobialbiomasscarbonandnitrogen
contentsofdifferenttreatments
05
!1# «¾º?:ëî¢Z[-.ÙC½É|?9K`giDD=¼îTVŠ
  ?9K`i»`ÜÓù%cNغ6Y,I
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Table2 Correlationcoefficientamongsoilchemicalproperties,enzymeactivityandmicrobialbiomasscarbonandnitrogen
pH
×\i
NH+4N
Ö\i
NO-3N
Ø:j
Available

àj
TotalP
JÁF
OM
=Áð

Catalase
¾¿½
Invertase
À½
Urease
*j

Phosp
hatase
?9K
`g
MBC
?9K
`i
MBN

ðU½
Catalase
0.597 -0.694 -0.557 0.736 0.398 -0.170 1 - - - - -
¾¿½
Invertase
0.592 0.098 -0.192 0.741 0.364 -0.202 0.506 1 - - - -
À½
Urease
0.616 -0.272 0.120 0.541 0.33 -0.538 0.604 0.697 1 - - -
*j

Phosphatase
0.725 -0.205 0.163 0.530 0.006 -0.543 0.567 0.770 0.937 1 - -
?9K
`g
MBC
0.463 -0.055 -0.153 0.572 0.110 -0.482 0.562 0.847 0.818 0.738 1 -
?9K
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MBN
0.452 -0.254 -0.365 0.723 0.361 -0.233 0.739 0.730 0.794 0.635 0.904 1
  ü:g˜…†ˆ‰(犘<0.05), g˜ú…†ˆ‰(犘<0.01)
Note:indicatesignificantcorrelationatthe0.05level,indicatesignificantcorrelationatthe0.01level
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