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Physiological and Biochemical Response of Fagopyrum dibotrys to High Temperature Stress and Evaluation of Their Heat Tolerance

高温对金荞麦生理生化特征的影响及其耐热性评价



全 文 :!24" !1#
 Vol.24  No.1
! " # $
ACTA AGRESTIA SINICA
   2016$  1%
  Jan.  2016
犱狅犻:10.11733/j.issn.10070435.2016.01.032
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:S541;Q945.78    ABCDE:A     AFG@:10070435(2016)01022204
犘犺狔狊犻狅犾狅犵犻犮犪犾犪狀犱犅犻狅犮犺犲犿犻犮犪犾犚犲狊狆狅狀狊犲狅犳犉犪犵狅狆狔狉狌犿犱犻犫狅狋狉狔狊狋狅
犎犻犵犺犜犲犿狆犲狉犪狋狌狉犲犛狋狉犲狊狊犪狀犱犈狏犪犾狌犪狋犻狅狀狅犳犜犺犲犻狉犎犲犪狋犜狅犾犲狉犪狀犮犲
ZHAOLili1,CHENChao1,DENGRong2,ZHAOLifen3,YANGFang4
(1.DepartmentofGrasslandScience,ColegeofAnimalScience,GuizhouUniversity,Guiyang,GuizhouProvince550025,China;
2.GuizhouInstituteofAnimalHusbandryandVeterinary,Guiyang,GuizhouProvince550005,China;3.GuizhouGrassSuperviseStation,
Guiyang,GuizhouProvince550002,China;4.LiupanshuiAgricultureCommittee,Liupanshui,GuizhouProvince553001,China)
犃犫狊狋狉犪犮狋:Toelucidatethephysiologicalandbiochemicalmechanismof犉.犱犻犫狅狋狉狔狊heattolerance,changes
ofphysiologicalandbiochemicalindexesineightgermplasmresoursesof犉.犱犻犫狅狋狉狔狊werestudiedunder
hightemperature.Theresultsshowedthattheelectricconductivity,MDAcontent,prolinecontentof犉.
犱犻犫狅狋狉狔狊increasedunderhightemperature.Solubleproteincontentincreasedatthebeginning,andthen
decreasedwiththetemperatureincreasing.Theactivitiesofsuperoxidedismutase(SOD),catalase(CAT)
andperoxidase(POD)increasedunderhightemperature.Theorderofheattoleranceofeightgermplasm
resourseswasasfolow:JQ6>JQ2>JQ3>JQ1>JQ8>JQ4>JQ5>JQ7.
犓犲狔狑狅狉犱狊:Fagopyrumdibotrys;Physiologicalandbiochemicalindex;Heattolerance
HIJK
:20141008;LMJK:20150320
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Authorforcorrespondence,Email:gzgyxgc3855218@163.com
  sÛUVJKTñ\、9ùep6‘šcd,nUVJKT¤`61F
[1]。
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Hara)¬é¬å9¾9ÐÑJK,ÞJtsTÎ
d6‹dM
[2]。
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eåY
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•*3“。
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Table1 Eightmaterialsof犉犪犵狅狆狔狉狌犿犱犻犫狅狋狉狔狊
l{
Order
ç”_
Source
ª«
/m
Altitude
9lxö
Habitatconditions
JQ1
yduÃè
Xiaobi,Guiyang
1071
Ø+
Growthinhilside
JQ2
”ˆºdy
Bianyang,Luodian
810
-_
Growthingrassland
JQ3
:Äs©y
Yongqing,Xifeng
980
Ø+
Growthinhilside
JQ4
YÒüù
Dafangcounty
1700

Growthonroadside
JQ5
‘sT/‰
Qixingguan,Bijie
1400
ãŠ
Growthonridge
JQ6
Qùë_è
Dongdi,Shuicheng
1625

Growthonroadside
JQ7
>”2“&ü
Zizhong,Neijiang,Sichuan
600
ãŠ
Growthonridge
JQ8
ց{y
Wangjiazhai,Nayong
1598
Ø+
Growthinhilside
1.3 lmno
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Ï
dºâ3²ßܸ€^GFTӚŽpD。
ºâ1:犝(狓犻)=(狓犻-狓犻犿犻狀)/(狓犻犿犪狓-狓犻犿犻狀) 
犻=l,2,3……m
ºâ2:犠犻=狆犻/∑犘犻 犻=1,2,3……n
ºâ3:犇 = ∑[犝(狓犻)·犠犻] 犻=1,2,3…
…n
‘&
:犝(狓犻)n!犻M̒=Tð¬më,狓犻
n@ß̒=k=
,狓犻犿犪狓6狓犻犿犻狀=¾n!犻MÌ
’=TÝY6Ýu
。犠犻 g˜!犻̒=L1
J̒=&T•«ÄA
;狆犻 n!犻M̒=T|}
Ý
。犇nŽpÓšÁ=。
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2.1 oQ¹C2¡Q
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(MDA)»
`©ÀÓùÛAíîníî

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ܸ€^GFˆD`"Ý

õJQ26JQ6)6 MDA
»`

õJQ6)©…†íî,45℃Óù2WdÝY。
JQ1,JQ56JQ8TˆD`"Ý6 MDA»`íì
tY
。JQ6TˆD`"Ý6 MDA»`íìÝu。
èë1Lã,¸ €^GFT†…W(Pro)6LÛ
ó¦
(SP)»`ÀÛA<ðóI6@。ÜGFPro
»`ÀÛAíês
,SP»`jês_€。
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ù2

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†€

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u

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。45℃Óù2,POD½É
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¹²|}Ýn85.030%)
cn¸€^GFŽpÁMTӚ»F

rg2)。Ì
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Fig.1 Theeffectofhightemperaturestressesonphysiologicalandbiochemicalindex
ü

6@;Çg˜@߇ý6@ÛA%غ…†
(犘<0.05)
Note:Differentlettersamongtreatmentsinthesamematerialsmeansignificantdifferenceatthe0.05level
`2 {·noP¹C2‘y>>r
Table2 Theprinciplecomponentanalysisof
differentindexesunderhightemperaturestress
»FIndexes
̒=
Principlecomponents
1 2
ˆD`"Ý
Relativeconductivity
0.733 -0.5048
eåY
MDA
0.9439 -0.3465
†…W
Proline
0.8624 0.7320
„g2
»FIndexes
̒=
Principlecomponents
1 2
Lۏó¦
Solubleprotein
0.2282 0.8518
QÁðKnð½SOD 0.9529 0.2832
=ÁðU½CAT 0.9460 0.0746
=ÁðK½POD 0.8334 0.198
|}Ý
Contributerate/%
67.448 17.582
¹²|}Ý
Cumulativecontributerate/%
67.448 85.030
422
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Table3 Thecomprehensiveevaluationof犉.犱犻犫狅狋狉狔狊underhightemperaturestress
‡ý
Materials
犡1 犡2 犝(1) 犝(2)
D
ValueofD
il
Order
JQ1 1205.580 200.038 0.973 0.121 0.797 4
JQ2 1206.740 230.582 0.983 0.791 0.943 2
JQ3 1208.662 226.694 1.000 0.706 0.939 3
JQ4 1148.362 208.062 0.470 0.297 0.434 6
JQ5 1120.765 194.515 0.228 0.000 0.181 7
JQ6 1205.328 240.103 0.971 1.000 0.977 1
JQ7 1094.832 195.436 0.000 0.020 0.004 8
JQ8 1172.557 197.528 0.683 0.066 0.555 5
3 ˆ‰Wp‰
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、MDA6Pro»`ÀÓùÛA
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Ÿ
[5];
nKŠsÛ
,¸
€^<=†…W
(Pro)íîó
¦F=mTQšA

ÐÑó¦F=mÎcsÛ1B

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[6]
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0efß#

Lۏó¦
(SP)ÞJt–T¥

§Bh…íîH.
›s5ÆQ»`
[7]。
BC&
,8M¸€^GFTSP»`ÀÛAíîjê
s_€

L35℃Óù2WdÝY,.2JK›
s¡N2pMó¦TgWQR

ó¦F»`„ê
sóI

ÛAíîÍ45℃2,ó¦FTš’cd)
ÆC"ó¦F”

Ý.JrN2Tcó¦F
»`€
[7]。
sÛÓùœ
,¸
€^GFTSOD,CAT6POD
½É£„íî

‚iɏÁDH.T-±
[8];35℃
Óù2
,SOD6CAT½ªÌ«ÐÑcd,45℃Óù
2
,3GÐѽˆö8aÐÑH.。»/0ef
ª¾Û
[8]
?D_xìí
(犕犲犱犻犮犪犵狅狊犪狋犻狏犪)6ª

[9]
?DQ§
(犗狉狔狕犪狊犪狋犻狏犪)T/0ef6@,
.L§6@JKGDsÛKŠÁ|/06@


ӚÁM¸€^GFTŽpkln
:JQ6>JQ2
>JQ3>JQ1>JQ8>JQ4>JQ5>JQ7。
”•AB
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9\VŠ
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O…Ó
,2000
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9ð»FTUV
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6qTUV
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