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作 者 ：梁宇斌;毕永红;刘国祥;胡征宇

期 刊 ：武汉植物学研究 2010年 28卷 1期 页码：43-48

关键词：脐橙；蜜橘；西柚；铜绿微囊藻；生长抑制；PSⅡ；

Keywords:Citrus sinensis, Citrus unshiu, Citrus grandis, Microcystis aeruginosa, Inhibitory effect, PSⅡ,

摘 要 ：

通过分析测定生物量、叶绿素a含量以及叶绿素荧光参数，研究了3种柑橘类果皮甲醇提取液对铜绿微囊藻生长的影响。结果表明，3种提取液都能有效抑制铜绿微囊藻的生长、叶绿素a合成与光合系统Ⅱ（PSⅡ）活性，并且抑制效果随着作用浓度增加而增强。3种提取液抑制作用强弱的顺序为：蜜橘>西柚>脐橙。当蜜橘皮提取液浓度大于1.10 g/L时，抑藻效果显著（P<0.05），培养9 d后对铜绿微囊藻生长的抑制率达到8.4%，且在实验期间抑制作用没有减弱。当脐橙皮与西柚皮提取液的浓度大于3.31 g/L时，抑藻效果显著（P<0.05），但培养5 d后抑制作用开始减弱。据此推测，3种柑橘类果皮提取液中存在一类或几类物质，能够抑制铜绿微囊藻的叶绿素a合成，降低PSⅡ活性，从而降低其光合作用效率，导致铜绿微囊藻的生长受到抑制。且这类物质能自然降解，随着作用时间的延长，其抑藻效果也逐渐消失。

Abstract:In this paper，the effect of methanol extract from three citrus peels on the growth of Microcystis aeruginosa PCC7806 was studied.The parameters，such as biomass，chlorophyll a concentration and chlorophyll a fluorescence，were used in the assay.The result showed that all the three extracts could inhibit the growth of M.aeruginosa effectively，as to the chlorophyll a concentration and the activity of PSⅡ.And the inhibitory ratio (IR) increased with the concentration of the extracts.The order of the inhibitory activity was as follows： Citrus unshiu>Citrus grandis>Citrus sinensis.In the mandarin group，the inhibitory effect was remarkable in the present of the concentration of the extract higher than 1.10 g/L (P<0.05)，which was kept in the whole period of experiment.And the IR of biomass is up to 86.4% after 9 days culture.When the extract concentration is higher than 3.31 g/L in navel orange and grapefruit groups，the inhibitory effect showed significant (P<0.05).However，the recovery happened after 5 days culture.It was deduced that there was some material in the citrus peels inhibiting the growth of M.aeruginosa by way of the inhibition of chlorophyll a synthesis and the activity of PSⅡ，which could both reduce the efficiency of the photosynthesis.And also the material could be degraded in natural conditions resulting in the inhibitory effects disappearing gradually with prolonged exposure.

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