---

作 者 ：余知和;曾昭清;张明涛

期 刊 ：武汉植物学研究 2009年 27卷 3期 页码：332-335

关键词：春兰；菌根真菌；分离；菌根显微结构；

Keywords:Cymbidium georingii, Mycorrhizal fungi, Isolation, Mycorrhizal microstructure,

摘 要 ：以盆栽春兰和野生春兰的根为材料,通过切片观察菌根真菌的分布特点和菌根的显微结构.同时采用组织分离方法从盆栽和野生春兰根分别分离得到6个和15个真菌菌株,分离菌株的出现频率分别为16.7%～50%和6.7%～73.3%,并由此判断CGP-3为盆栽春兰菌根的优势菌种,CGW-3为野生春兰菌根的优势菌种.经形态学观察将其初步分为6个分类单元,其中CGP-2和CGW-2分别具有拟日本曲霉(Aspergillus japonicus-like)和拟木霉属(Trichodermasp.-like)的形态特征.此外,对兰科植物与内生真菌间的专一性进行了讨论.

Abstract:The distribution patterns of mycorrhizal fungi and the microstructure of mycorrhizae in pot cultured and wild Cymbidium georingii were investigated and observed.Six strains of mycorrhizal fungi were isolated and obtained from the mycorrhizae in pot cultured C.georingii and fifteen from the wild ones,which their frequency was 16.7%-50% and 6.7%-73.3%,respectively.According to this data of frequency,the strain CGP-3 would be dominant one of pot cultured and CGW-3 would be predominance in wild C.georingii.On the basis of morphological observation all of 21 strains isolated were identified into six taxa,two of them were similar morphological appearances as Aspergillus japonicus-like and Trichoderma sp.-like.By the way,the specificity between the endomycorrhizal fungi and arethusa was also discussed briefly.

---